Innovative adhesive bio-films were developed for antimicrobial wound bandages. The bio-films are prepared by blending natural polymers such as sodium carboxymethyl cellulose and sodium alginate with a surfactant as Tween(80) and loaded with Linezolid drug as antimicrobial agents. In this paper, polymeric films were prepared by casting film-forming method. Prefatory studies comprised XRD, FTIR, UV/vis spectroscopy, and SEM to specify lattice structure, interactions, optical properties, and morphology. Antibacterial analysis was also performed by agar well diffusion tests. The effect of bio-film composition on E.coli, P.aeuroginosa, S.aureus, B.subtilis standard bacteria, and C.albicans pathogenic fungus was studied and compared with biomaterial film without Linezolid drug. The in vitro antibacterial assay of bio-films formulations was assessed in bacterial and fungal strains, showing a significant activity over time rather than control bio-film. Finally, the preparation of biomaterial films loaded with Linezolid drug (antimicrobial wound dressings) is an environmentally friendly solution that may contribute to the development of wound healing.