Bioactivity Assay of an Isolated Marine Fusarium sps

Abstract

Marine fungi are the potential and promising sources for biologically active secondary metabolite productions. Secondary metabolites are the chemical compounds that are produced during the stationary phase of the organism. Many years of study revealed that fungi are excellent sources for novel bioactive secondary metabolites. In the present study fungi were isolated from marine water samples and serial dilution method was performed to isolate single colonies. Later microscopic and macroscopic observations were done to identify the organism. Organisms are inoculated into medium for production of secondary metabolites. One week later ethyl acetate is added to the broth in 1:1 ratio and kept for incubation in shaker. Solvent is separated by separating funnel and solvent is extracted by rotavapour to collect the crude extract. Crude extract is dissolved in DMSO and further tested for biological activities. The Fungi was identified as Fusarium species and its secondary metabolites showed potential antimicrobial activity. The present work was attempted to isolate organisms which are capable of producing efficient antibacterial and antifungal compounds. Further we identified the organism as Fusarium sp. The future scope of this work is to isolate these biologically active compounds to use in pharmaceutical applications.

First report of Fusarium oxysporum species complex infection in zebrafish culturing system

“…Most of FOCS strains are ubiquitous in soil and also have been found in wide range of aquatic ecosystems including marine (Swathi et al . ) and freshwater (Palmero et al . ).…”

“…Fusarium oxysporum is a species complex (FOSC) which includes widely dispersed pathogenic strains to many hosts. Most of FOCS strains are ubiquitous in soil and also have been found in wide range of aquatic ecosystems including marine (Swathi et al 2013) and freshwater (Palmero et al 2009). Moreover, several F. oxysporum strains have been identified as human pathogens causing localized or disseminated infections associated with immune compromised patients (O'Donnell et al 2004).…”

Potential Reasons for Prevalence of Fusarium Wilt in Oriental Melon in Korea

“…F. oxysporum is a genetically heterogeneous polytypic morphospecies ( O’Donnel and Cigelnik, 1997 ; Waalwijk et al, 1996 ), and the taxon has been regarded as a species complex whose strains are widely distributed in soil and also found in wide range of aquatic ecosystems ( Gordon and Martyn, 1997 ; Palmero et al, 2009 ; Swathi et al, 2013 ). Also the F. oxysporum isolates distributed in the oriental melon in the present study showed the high morphological variations and high diversifications within the same clade of F. oxysporum in the phylogenetic analysis based on EF-1α gene sequences.…”

Antimicrobial, Cytotoxic, Antiviral Effects, and Spectroscopic Characterization of Metabolites Produced by Fusarium oxysporum YP9B

“…Mushrooms have broad biodiversity. Various interdisciplinary studies were ongoing on the secondary metabolites secreted by nonpathogenic Fusarium species such as F. oxysporum, Fusarium graminearum, and Fusarium moniliforme [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Many studies have focused on the toxicity and diversity of Fusarium species on cereals [20][21][22].…”

“…It is well known that Fusarium species produce trichothecene mycotoxins [4], but these species are also known to have as other compounds; pigments, antibiotics, phytotoxins, etc. [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]27].…”