Marine fungi are the potential and promising sources for biologically active secondary metabolite productions. Secondary metabolites are the chemical compounds that are produced during the stationary phase of the organism. Many years of study revealed that fungi are excellent sources for novel bioactive secondary metabolites. In the present study fungi were isolated from marine water samples and serial dilution method was performed to isolate single colonies. Later microscopic and macroscopic observations were done to identify the organism. Organisms are inoculated into medium for production of secondary metabolites. One week later ethyl acetate is added to the broth in 1:1 ratio and kept for incubation in shaker. Solvent is separated by separating funnel and solvent is extracted by rotavapour to collect the crude extract. Crude extract is dissolved in DMSO and further tested for biological activities. The Fungi was identified as Fusarium species and its secondary metabolites showed potential antimicrobial activity. The present work was attempted to isolate organisms which are capable of producing efficient antibacterial and antifungal compounds. Further we identified the organism as Fusarium sp. The future scope of this work is to isolate these biologically active compounds to use in pharmaceutical applications.

No abstract

Marine micro flora has a wide range of importance in view of its diversity and distribution. Fungi have a large number species, and each species has their own specified characters. The main objective of the work is to isolate and identify marine fungi collected from different habitats. Methodology and results: The samples were collected and stored in tight sterile bottles and transferred to lab carefully. Fungal isolates were screened from marine water sample at different dilutions and its genus were identified and studied. All the colonies of each plate are counted by colony counter, further those pure colonies were isolated and studies are carried out to identify them. The site for the isolate collection had been studied, the regions in this area is highly diversified with different marine ecosystems. The water sample is collected at a distance 1 km away from the edges of beaches. Thus sample is analysed for taxonomical evaluation and species identification. The taxonomical study evaluated nearly for 15 spp. out of 22 isolates. Among those some isolates were identified as Aspergillus sp., Curvularia sp., Fusarium sp., and Microascus sp. These species have more diversified in its spore formation and its arrangement. Conclusion, significance and impact study: Throughout the study we tried to list out all the methodologies that will help a fresh researcher to identify a marine isolate through microscopic and macroscopic studies. Thus the coastal line of Andhra Pradesh had significant studies in isolating a valuable sp. for further development. Our study reveals, among all isolates Microascus sp., had provoked a good literature for the future research and till now its taxonomical studies are less evaluated. This would be encouraged for scientists to identify rare species to increase the evolution.

To investigate the phytochemical, antimicrobial and antioxidant potentials of Bryonia epigaea (Rottler). The study was performed by using various in-vitro methods such as 1, 1 Diphenyl 2 picryl hydrazyl (DPPH), and Agar well diffusion method for different concentrations of methanolic extracts. Phytochemical constituents of the three extracts (Hexane, Acetone and Methanol) were determined. Total Phenol content was determined by Folin Ciocalteu Method. Yield of extract was determined by caliculating the mass of plant material before minusing the mass of the plant material after the extraction process. Extracts of Bryonia epigaea (Rottler) contained saponins, flavonoids, steroids, tannins, alkaloids, coumarins, phenols and reducing sugars. The antimicrobial activity was moderate. Methanol extract showed good antibacterial activity at 100 mg/ml concentration while antifungal activity against four significant pathogens was moderate. Bryonia epigaea (Rottler) methanol extract exerted significant antioxidant activity and dose dependent effect. The Results showed that the methanol extract showed high amount of antioxidant compounds and exhibit significant antioxidant activity. The antioxidant activity was found to be increased with the concentration of the compound. In the present research work we tried to find out the bioactive properties of ancient medicinal plant Bryonia epigaea (Rottler). The phytochemical, antimicrobial and antioxidant properties have been discussed.