Marine micro flora has a wide range of importance in view of its diversity and distribution. Fungi have a large number species, and each species has their own specified characters. The main objective of the work is to isolate and identify marine fungi collected from different habitats. Methodology and results: The samples were collected and stored in tight sterile bottles and transferred to lab carefully. Fungal isolates were screened from marine water sample at different dilutions and its genus were identified and studied. All the colonies of each plate are counted by colony counter, further those pure colonies were isolated and studies are carried out to identify them. The site for the isolate collection had been studied, the regions in this area is highly diversified with different marine ecosystems. The water sample is collected at a distance 1 km away from the edges of beaches. Thus sample is analysed for taxonomical evaluation and species identification. The taxonomical study evaluated nearly for 15 spp. out of 22 isolates. Among those some isolates were identified as Aspergillus sp., Curvularia sp., Fusarium sp., and Microascus sp. These species have more diversified in its spore formation and its arrangement. Conclusion, significance and impact study: Throughout the study we tried to list out all the methodologies that will help a fresh researcher to identify a marine isolate through microscopic and macroscopic studies. Thus the coastal line of Andhra Pradesh had significant studies in isolating a valuable sp. for further development. Our study reveals, among all isolates Microascus sp., had provoked a good literature for the future research and till now its taxonomical studies are less evaluated. This would be encouraged for scientists to identify rare species to increase the evolution.