Bioactivity characterization of Lactobacillus strains isolated from dairy products

Abstract

This study aimed to find candidate strains of Lactobacillus isolated from sheep dairy products (yogurt and ewe colostrum) with probiotic and anticancer activity. A total of 100 samples were randomly collected from yogurt and colostrum and 125 lactic acid bacteria were isolated. Of these, 17 Lactobacillus strains belonging to five species (L. delbrueckii, L. plantarum, L. rhamnosus, L. paracasei, and L. casei) were identified. L. plantarum 17C and 13C, which isolated from colostrums, demonstrated remarkable results such as resistant to low pH and high concentrations of bile salts, susceptible to some antibiotics and good antimicrobial activity that candidate them as potential probiotics. Seven strains (1C, 5C, 12C, 13C, 17C, 7M, and 40M), the most resistant to simulated digestion, were further investigated to evaluate their capability to adhere to human intestinal Caco-2 cells. L. plantarum 17C was the most adherent strain. The bioactivity assessment of L. plantarum 17C showed anticancer effects via the induction of apoptosis on HT-29 human cancer cells and negligible side effects on one human epithelial normal cell line (FHs 74). The metabolites produced by this strain can be used as alternative pharmaceutical compounds with promising therapeutic indices because they are not cytotoxic to normal mammalian cells.

Probiotic potentials of Lactobacillus plantarum isolated from fermented durian (Tempoyak), a Malaysian traditional condiment

“…Our findings are also in accordance with a previous study by Haghshenas et al. () which demonstrated that secreted metabolites of L. plantarum 17C isolated from ewe colostrum exhibited antiproliferative effect on HT‐29 cell line. Antiproliferative activity of probiotic strains on colon cancer cells could be due to the presence of EPS (Sadeghi‐Aliabadi, Mohammadi, Fazeli, & Mirlohi, ).…”

“…This is in parallel to the findings reported by Er, Koparal, and Kivanc (2015) that Pediococcus pentosaceus, L. plantarum and Weissella confusa isolated from fermented meat were able to inhibit the growth of colon cancer cells in a dose-dependent manner and, more interestingly, cell-free filtrate of L. plantarum also showed stronger effects compared with the other two strains. Our findings are also in accordance with a previous study by Haghshenas et al (2015) which demonstrated that secreted metabolites of L. plantarum 17C isolated from ewe colostrum exhibited antiproliferative effect on HT-29 cell line. Antiproliferative activity of probiotic strains on colon cancer cells could be due to the presence of EPS (Sadeghi-Aliabadi, Mohammadi, Fazeli, & Mirlohi, 2014).…”

Influence of natural dairy probiotics on health

“…It has been proved that their effect is associated with a reduction of proliferative activity of colon cancer cell lines HT-29 and Caco-2, and the aforementioned reduction in ErbB-2 and ErbB-3 gene expression [37]. The probiotic strain L. plantarum 17C has also been tested for antiproliferative activity by Haghshenas et al [38]. In this study, its antitumour properties and ability to regulate apoptosis -a process significantly disturbed in neogenesis -have been proved [38].…”

Combining Prebiotic and Mixture of Freeze Dried Probiotics to Develop Synbiotics that Induce Apoptosis in Human Colon Tumor Cells

“…Also, Haghshenas et al (2015) studied the effect of L. plantarum 17C on HT-29 cell viability, HT-29 cells were exposed to the supernatant produced during the late stationary growth phase of L. plantarum 17C and analyzed by fluorescent microscope and found a significant number of apoptotic cells after 24 h of incubation with 50 µg/ml of L. plantarum 17C supernatant. et al (2015) reported that the supernatants of L. acidophilus ATCC 4356 and L. casei ATCC 39392 decreased CaCo-2 cell proliferarion and increased cell apoptosis.…”