Bioactivity Ingredients of <i>Chaenomeles speciosa</i> against Microbes: Characterization by LC-MS and Activity Evaluation

Wang, Zhaojie; Jin, Danni; Zhou, Ying; Sang, Xu-Yan; Zhu, Yanyan; He, Yun; Xie, Tian-Zhen; Dai, Zhi; Zhao, Yun‐Li; Luo, Xiao‐Dong

doi:10.1021/acs.jafc.1c00298

Cited by 33 publications

(19 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of metabolites suggest that C. speciosa fruits were an excellent source of flavonoids, alkaloids, terpenoids and phenols metabolites. In this study, the numbers of metabolites were greater than in previous research results, which suggests that in previous decades, metabolites were mainly detected and identified by using GC-MS, CC, LC-MS and HPLC [ 5 , 6 , 8 , 9 , 13 ].…”

Section: Resultscontrasting

confidence: 69%

“…These findings explain why YN has higher antioxidant activity [ 9 ]. Furthermore, we found abundant trimethoprim had antibacterial and antimalarial properties, which is a powerful supplement to Luo’s antibacterial experiment of C. speciosa fruits [ 5 ].…”

Section: Resultsmentioning

confidence: 99%

“…Previous studies have confirmed that C. speciosa fruits have many antioxidants and different phytochemicals for anticancer [ 2 ], antioxidant [ 3 ], antiviral [ 4 ], antibacterial properties [ 5 , 6 ], anti-inflammation [ 4 ], antihyperlipidemic [ 3 ], antihyperglycemic [ 3 ] and anti-Parkinson properties [ 7 ]. In addition, the Pharmacopoeia of the People’s Republic of China contains two triterpenoid acids, namely oleanolic acid and ursolic acid, which are legal compounds.…”

Section: Introductionmentioning

confidence: 99%

“…C. speciosa metabolites have been analyzed by gas chromatography–mass spectrometry (GC-MS) [ 9 ], column chromatography (CC) [ 13 ], liquid chromatography–mass spectrometry (LC-MS) [ 5 ] and high-performance liquid chromatography (HPLC) [ 8 ] in recent decades. Previous researchers primarily focused on organic acids, triterpenes, phenolic acids, flavonoids, coumarins, triterpenoids saponins, free amino acids, essential oils and alkaloids [ 14 ], which have a comparatively high content in fruits and are relatively easy to detect.…”

Section: Introductionmentioning

confidence: 99%

“…However, there is a lack of published reports illustrating other compounds containing phytohormones, phenylpropanoids, vitamins, carbohydrates, alcohols and so on. Meanwhile, many previous studies used high-temperature or sun-dried samples as experimental materials [ 5 , 9 ], which could cause the loss of active ingredients that are not heat resistant. Moreover, because the relatively old mass spectrometry database is relatively old, there are very few types of substances to detect.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Comparative Metabolomics Study of Chaenomeles speciosa (Sweet) Nakai from Different Geographical Regions

et al. 2022

Foods

View full text Add to dashboard Cite

Chaenomeles speciosa (Sweet) Nakai (C. speciosa) is not only a Chinese herbal medicine but also a functional food widely planted in China. Its fruits are used to treat many diseases or can be processed into food products. This study aims to find key metabolic components, distinguish the differences between geographical regions and find more medicinal and edible values of C. speciosa fruits. We used ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC-MS/MS) and widely targeted metabolomics analysis to reveal key and differential metabolites. We identified 974 metabolites and screened 548 differential metabolites from 8 regions. We selected significantly high-content differential metabolites to visualize a regional biomarker map. Comparative analysis showed Yunnan had the highest content of total flavonoids, the highest amounts of compounds related to disease resistance and drug targets and the most significant difference from the other regions according to the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform database, a unique platform for studying the systematic pharmacology of Chinese herbal medicine and capturing the relationship between drugs, targets and diseases. We used oral bioavailability (OB) ≥ 30% and drug likeness (DL) ≥ 0.18 as the selection criteria and found 101 key active metabolites, which suggests that C. speciosa fruits were rich in healthy metabolites. These results provide valuable information for the development of C. speciosa.

show abstract

Section: Resultscontrasting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparative Metabolomics Study of Chaenomeles speciosa (Sweet) Nakai from Different Geographical Regions

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

Phytochemical Profiling and Compound Isolation of Cissus rhombifolia Vahl. Leaves Aqueous Methanolic Extract with the Evaluation of Its Anti‐Inflammatory Effect Using Lipopolysaccharide‐Induced Inflammation in RAW 264.7 Cells

Raslan¹,

Mounier²

2023

Chemistry & Biodiversity

View full text Add to dashboard Cite

The inflammatory disorders represent a serious health issue. Certain Cissus species possess anti-inflammatory effect. Cissus rhombifolia Vahl. leaves' anti-inflammatory activities and phytoconstituents are poorly characterized. In this study, 38 constituents were tentatively characterized in Cissus rhombifolia Vahl. leaves' aqueous methanolic extract (CRLE) using high-performance liquid chromatography combined with mass spectrometry (HPLC/MS) and Proton Nuclear Magnetic Resonance ( 1 H-NMR). Myricetin, β-amyrin, and alliospiroside A, were isolated from CRLE using column chromatography. The anti-inflammatory effect of CRLE and its isolated compounds were studied in lipopolysaccharide (LPS)-induced RAW 264.7 cells. 3-(4,5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT as-say) was used to assess how CRLE and its isolated compounds affected cell viability. Further, its effects on the production of intracellular NO, and inflammatory cytokines cyclooxygenase-2 (COX-2), tumor necrosis factor alpha (TNF-α), and interleukin 6 (IL-6) were assessed by the Griess test, and cytokine enzymelinked immunosorbent assays, respectively. CRLE and its isolated compounds, myricetin, β-amyrin, and alliospiroside A decreased the NO production. Western blotting was performed to assess the protein expression levels of the inflammatory cytokines inducible nitric oxide synthase (iNOS). Alliospiroside A downregulated IL-6, TNF-α, and COX-2 and inhibited the expression of iNOS. CRLE and its compounds represent effective alternative candidate to treat inflammatory diseases.

show abstract

Biphenyls and dibenzofurans of the rosaceous subtribe Malinae and their role as phytoalexins

Busnena,

Beerhues,

Liu

2023

Planta

View full text Add to dashboard Cite

Main conclusion Biphenyl and dibenzofuran phytoalexins are differentially distributed among species of the rosaceous subtribe Malinae, which includes apple and pear, and exhibit varying inhibitory activity against phytopathogenic microorganisms. Abstract Biphenyls and dibenzofurans are specialized metabolites, which are formed in species of the rosaceous subtribe Malinae upon elicitation by biotic and abiotic inducers. The subtribe Malinae (previously Pyrinae) comprises approximately 1000 species, which include economically important fruit trees such as apple and pear. The present review summarizes the current status of knowledge of biphenyls and dibenzofurans in the Malinae, mainly focusing on their role as phytoalexins. To date, 46 biphenyls and 41 dibenzofurans have been detected in 44 Malinae species. Structurally, 54 simple molecules, 23 glycosidic compounds and 10 miscellaneous structures were identified. Functionally, 21 biphenyls and 21 dibenzofurans were demonstrated to be phytoalexins. Furthermore, their distribution in species of the Malinae, inhibitory activities against phytopathogens, and structure–activity relationships were studied. The most widely distributed phytoalexins of the Malinae are the three biphenyls aucuparin (3), 2ʹ-methoxyaucuparin (7), and 4ʹ-methoxyaucuparin (9) and the three dibenzofurans α-cotonefuran (47), γ-cotonefuran (49), and eriobofuran (53). The formation of biphenyl and dibenzofuran phytoalexins appears to be an essential defense weapon of the Malinae against various stresses. Manipulating phytoalexin formation may enhance the disease resistance in economically important fruit trees. However, this approach requires an extensive understanding of how the compounds are formed. Although the biosynthesis of biphenyls was partially elucidated, formation of dibenzofurans remains largely unclear. Thus, further efforts have to be made to gain deeper insight into the distribution, function, and metabolism of biphenyls and dibenzofurans in the Malinae.

show abstract

Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation

Cited by 33 publications

References 35 publications

Comparative Metabolomics Study of Chaenomeles speciosa (Sweet) Nakai from Different Geographical Regions

Comparative Metabolomics Study of Chaenomeles speciosa (Sweet) Nakai from Different Geographical Regions

Phytochemical Profiling and Compound Isolation of Cissus rhombifolia Vahl. Leaves Aqueous Methanolic Extract with the Evaluation of Its Anti‐Inflammatory Effect Using Lipopolysaccharide‐Induced Inflammation in RAW 264.7 Cells

Biphenyls and dibenzofurans of the rosaceous subtribe Malinae and their role as phytoalexins

Contact Info

Product

Resources

About