Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk Pinctada maxima in three mammalian cell types: fibroblasts, bone marrow stromal cells and osteoblasts

Mouriès, Lucilia Pereira; Almeida, Maria; Milet, Christian; Berland, Sophie; Lopez, Évelyne

doi:10.1016/s1096-4959(01)00524-3

Cited by 101 publications

(42 citation statements)

References 54 publications

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“…In mollusc shells, the aragonite and the protein matrix form a composite material (mineral matrix + organic matrix) called nacre. Taking into account that the mechanisms behind the construction of the different biomineralized tissues occur in similar fashion, it seems coherent to seek out materials that can substitute or induce the regeneration of bone tissue in other biomineralizing organisms, and this explains the attention given to corals and molluscs with shells 5,6,[20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36] . Thus, this work has sought, through the gathering and comparison of the characteristics of clinical interest, to find in the residues from malacoculture the potential for obtaining an industrial input of low ecological/economic impact.…”

Section: Introductionmentioning

confidence: 99%

“…Nacre and biogenic aragonite are two materials of 25 , without inflammation and/or fibrous formations. In vivo and in vitro studies indicate the nacre as a biocompatible, biodegradable and osteocondutive material, which may attract and activate bone marrow stem cells and osteoblasts; the same was observed in biogenic aragonite [22][23][24][25][26][27][28][29][30][31][32][33][34][35] . The mechanical and tribological properties -as tensile, compression and bending strength, besides resistance to abrasion -of the nacre, comparatively better than that of pure aragonite, are due to its hierarchical structure of micrometric hexagonal tablets with mineral bridges and interlocking surfaces 36,[38][39][40][41][42] and cemented in organic matrix.…”

Section: Introductionmentioning

confidence: 99%

“…The mechanical and tribological properties -as tensile, compression and bending strength, besides resistance to abrasion -of the nacre, comparatively better than that of pure aragonite, are due to its hierarchical structure of micrometric hexagonal tablets with mineral bridges and interlocking surfaces 36,[38][39][40][41][42] and cemented in organic matrix. Considering that to this matrix are also attributed mineralizing and osteogenic properties 22,[30][31][32][33][34] , the hypothesis raised initially was that the nacre would present more pronounced osteogenic properties than the aragonite, disregarding this study any differences in the mechanical properties.…”

Section: Introductionmentioning

confidence: 99%

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Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Almeida¹,

Silva²,

Nakamura³

et al. 2015

Mat. Res.

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Aragonite is a metastable polymorph of calcium carbonate found in mollusk's shells, appearing in tiles and prismatic columns, cemented in a protein matrix -mainly proteins -that acts as a framework on which the aragonite is nucleated forming nacre, besides selecting the morphology of the nucleated cristaline phase. The presence of the mineralyzing organic matrix may affect osteoinductive properties of biogenic aragonite, hypothesis tested by combinated tests, comparing viability and bioactivity of biomineralizated aragonite and nacre. Bioactivity was observed by deposition of Ca-P (presumably calcium phosphate) on the surface of samples immersed in Simulated Body Fluid; biocompatibility was verified by adhesion with VERO cells; cytotoxicity and alkaline phosphatase activity assays were performed with human adipose stem cells (hASC). Samples were characterized by scanning electron microscopy and X-ray diffraction. Both materials showed similar behaviour on bioactivity assay; in contrast, exhibited different behaviours in the presence of hASC.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Almeida¹,

Silva²,

Nakamura³

et al. 2015

Mat. Res.

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“…11,12 Besides the highly advantageous mechanical properties, the nacre is biologically compatible with bone tissues. 13,14 Because of the promising mechanical and biocompatibility properties, nacre is a prime candidate for potential tissue engineering applications. For example, recent animal in vivo studies by Berland et al have shown good cellular interaction between nacre implants and bone forming cells.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of a bone like composite material derived from waste pearl oyster shells for potential bone tissue bioengineering applications

2017

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Background: Hydroxyapatite is generally considered a viable substitute for bone in a number of medical procedures such as bone repair, bone augmentation and coating metal implants. Unfortunately, hydroxyapatite has poor mechanical properties that make it unsuitable for many load bearing applications.Methods: In the present work various grades of finely crushed Pinctada maxima (pearl oyster shell) were combined with a nanometer scale hydroxyapatite powder to form novel composite materials. A comparative study was made between the various powder based composites synthesized. The crystalline structure and morphology of the various powder based composites were investigated using X-ray diffraction and field emission scanning electron microscopy. The composite materials were also evaluated and characterized.Results: Manufactured hydroxyapatite powders were composed of crystalline spherical/granular particles with a mean size of 30 nm. Also produced were hydroxyapatite and finely crushed calcium carbonate from Pinctada maxima (pearl oyster shell) powder mixtures. Hydroxyapatite coatings produced on Pinctada maxima nacre substrates were investigated and their surface characteristics reported.Conclusions: Pinctada maxima nacre pre-treated with sodium hypo chlorate before hydroxyapatite deposition produced a superior coating and could be used for bone tissue engineering. But further in vitro and in vivo studies are needed to validate the biocompatibility and long term stability of this composite coating.

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“…Many SMPs have been identified and biochemically characterised with a wide range of potential functions such as exertion of signalling activities towards the calcifying mantle epithelium, interaction with minerals and catalysis of enzymatic reactions (Marin and Luquet 2004, Marin et al 2008. Molluscan shell extracts also induce osteoblast differentiation and stimulate the synthesis of extracellular matrix components of fibroblast in mammalian cell cultures (Pereira Mouriès et al 2002, Rousseau et al 2003, Chaturvedi et al 2013, Latire et al 2014.…”

Section: The Mantle Appears To Use Conserved Developmental Regulatorsmentioning

confidence: 99%

Investigation of gene family evolution and the molecular basis of shell formation in molluscs

Aguilera¹

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Since the emergence of shell-bearing molluscs in the Early Cambrian, diverse shell forms have evolved. Modern molluscs are renowned for a highly complex and robust shell, which is the product of the orchestrated expression of genes and secretion of a large number of proteins and other macromolecules from the epithelium of a specialised organ called the mantle. Molluscan shells display remarkable morphological diversity, structure and ornamentation, however the molecular mechanisms underlying the evolution and formation of the shell remain largely unknown. In this thesis, I seek to investigate the nature of the mantle transcriptome focussing on the timing of origin of genes expressed in the mantle and the evolutionary history of gene families that encode secreted proteins in representatives of bivalve and gastropod species.First, by comparative transcriptomics of the mantle, I determined the origin and evolution of gene families expressed in this organ. Gene origin analyses show that most genes expressed in the mantle are taxon-restricted innovations (i.e. unique to a particular species), although a large number of genes arose along the stems leading to the bilaterian and molluscan last common ancestors. Focussing on gene families that encode secreted proteins that are likely to be embedded within the shell and/or to regulate shell construction, I found that these are comprised of both ancient and lineage-specific gene families. The evolution of these gene families is highly dynamic with prolific gene family gains and losses over evolutionary time. By comparing sequences, I also detected indications of positively selected gene families over molluscan evolution. These gene families show unique patterns of enrichment of protein domains, and presumably molecular functions, that are taxa-specific, suggesting that bivalves and gastropods use almost completely different secretory repertoire to construct their shells.I then focused on an ancient gene family expressed in the molluscan mantles -the tyrosinase gene family -to understand how a conserved gene family has been co-opted into the biomineralisation pathway. I found no evidence of large-scale expansion of tyrosinase genes in gastropods, whereas tyrosinase genes in bivalves have undergone substantial independent gene expansions in at least two lineages (Pinctada spp. and Crassostrea gigas), and that the resulting gene duplicates have been co-opted into the mantle gene regulatory network. Many of the tyrosinase genes are found in clusters within the genomes and are expressed at relatively high levels in the mantle. Detailed 3 comparisons of tyrosinase gene expression in different regions of the mantle in two closely related pearl oysters, P. maxima and P. margaritifera, reveal that recently evolved orthologous genes have unique expression profiles across the mantle with high expression levels at the distal mantle edge, suggesting roles in colouration and/or prismatic shell layer construction. Differences in expression levels are consistent with the rapid evo...

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Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk Pinctada maxima in three mammalian cell types: fibroblasts, bone marrow stromal cells and osteoblasts

Cited by 101 publications

References 54 publications

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Nacre Compared to Aragonite as a Bone Substitute: Evaluation of Bioactivity and Biocompatibility

Synthesis of a bone like composite material derived from waste pearl oyster shells for potential bone tissue bioengineering applications

Investigation of gene family evolution and the molecular basis of shell formation in molluscs

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