Bioadhesive, non-drug-loaded nanoparticles as modulators of candidal adherence to buccal epithelial cells: a potentially novel prophylaxis for candidosis

McCarron, Paul A.; Donnelly, Ryan F.; Canning, Paul; McGovern, James G.; Jones, David S.

doi:10.1016/j.biomaterials.2003.09.022

Cited by 32 publications

(20 citation statements)

References 34 publications

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“…Even though several antifungal agents have been used for treating OC, high concentration of these substances may cause improvement of resistant Candida strains, several side effects, and drug-drug interactions. [1][2][3][4][5] The antifungal agent fluconazole (FLZ) is one of the most important active agents in the site-specific treatment of OC, as reported by several researches. 6,7 It is a synthetic…”

Section: Introductionmentioning

confidence: 99%

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Rençber¹,

Karavana²,

Yılmaz³

et al. 2016

IJN

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This study aimed to develop a suitable buccal mucoadhesive nanoparticle (NP) formulation containing fluconazole for the local treatment of oral candidiasis. The suitability of the prepared formulations was assessed by means of particle size (PS), polydispersity index, and zeta potential measurements, morphology analysis, mucoadhesion studies, drug entrapment efficiency (EE), in vitro drug release, and stability studies. Based on the optimum NP formulation, ex vivo drug diffusion and in vitro cytotoxicity studies were performed. Besides, evaluation of the antifungal effect of the optimum formulation was evaluated using agar diffusion method, fungicidal activity-related in vitro release study, and time-dependent fungicidal activity. The effect of the optimum NP formulation on the healing of oral candidiasis was investigated in an animal model, which was employed for the first time in this study. The zeta potential, mucoadhesion, and in vitro drug release studies of various NP formulations revealed that chitosan-coated NP formulation containing EUDRAGIT ® RS 2.5% had superior properties than other formulations. Concerning the stability study of the selected formulation, the formulation was found to be stable for 6 months. During the ex vivo drug diffusion study, no drug was found in receptor phase, and this is an indication of local effect. The in vitro antifungal activity studies showed the in vitro efficacy of the NP against Candida albicans for an extended period. Also, the formulation had no cytotoxic effect at the tested concentration. For the in vivo experiments, infected rabbits were successfully treated with local administration of the optimum NP formulation once a day. This study has shown that the mucoadhesive NP formulation containing fluconazole is a promising candidate with once-a-day application for the local treatment of oral candidiasis.

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Section: Introductionmentioning

confidence: 99%

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Rençber¹,

Karavana²,

Yılmaz³

et al. 2016

IJN

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“…This potential interaction will be important for occupational exposures to nanoparticles as well as in nanomedicine, where development of novel approaches for treatment of invasive infections encompasses new delivery systems including nanoparticles/nanospheres that can substantially modulate the pharmacokinetics of existing compounds, and may also be useful to enhance the delivery of agents to sites of infection (5). For example, adherence of microorganisms to cells represents the initial step in the establishment of infection and, accordingly, modification of this step using nanoparticles with bioadhesive properties represents a method by which the incidence of infection may be manipulated (6).…”

mentioning

confidence: 99%

Sequential Exposure to Carbon Nanotubes and Bacteria Enhances Pulmonary Inflammation and Infectivity

Shvedova¹,

Fabisiak²,

Kisin³

et al. 2008

Am J Respir Cell Mol Biol

164

134

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Carbon nanotubes (CNT), with their applications in industry and medicine, may lead to new risks to human health. CNT induce a robust pulmonary inflammation and oxidative stress in rodents. Realistic exposures to CNT may occur in conjunction with other pathogenic impacts (microbial infections) and trigger enhanced responses. We evaluated interactions between pharyngeal aspiration of singlewalled CNT (SWCNT) and bacterial pulmonary infection of C57BL/6 mice with Listeria monocytogenes (LM). Mice were given SWCNT (0, 10, and 40 mg/mouse) and 3 days later were exposed to LM (10 3 bacteria/ mouse). Sequential exposure to SWCNT/LM amplified lung inflammation and collagen formation. Despite this robust inflammatory response, SWCNT pre-exposure significantly decreased the pulmonary clearance of LM-exposed mice measured 3 to 7 days after microbial infection versus PBS/LM-treated mice. Decreased bacterial clearance in SWCNT-pre-exposed mice was associated with decreased phagocytosis of bacteria by macrophages and a decrease in nitric oxide production by these phagocytes. Pre-incubation of naïve alveolar macrophages with SWCNT in vitro also resulted in decreased nitric oxide generation and suppressed phagocytizing activity toward LM. Failure of SWCNT-exposed mice to clear LM led to a continued elevation in nearly all major chemokines and acute phase cytokines into the later course of infection. In SWCNT/LM-exposed mice, bronchoalveolar lavage neutrophils, alveolar macrophages, and lymphocytes, as well as lactate dehydrogenase level, were increased compared with mice exposed to SWCNT or LM alone. In conclusion, enhanced acute inflammation and pulmonary injury with delayed bacterial clearance after SWCNT exposure may lead to increased susceptibility to lung infection in exposed populations.

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“…Apparently, the increased hydrophobicity of mutant cells may be caused by structural components of the cell wall that do not play an essential role in the biofilm formation. A reduced adherence, known to represent the initial step in the establishment of infection, associated with increased CSH has been also reported with nanoparticle-treated blastospores of C. albicans (McCarron et al 2004). The E 463 K amino-acid substitution in Pel1p results in anionic phospholipid deficiency and pleiotropic phenotype.…”

Section: Necrmentioning

confidence: 92%

Molecular and phenotypic analysis of mutations causing anionic phospholipid deficiency in closely related yeast species

et al. 2009

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The pel1 mutation in Saccharomyces cerevisiae and the Cgpgs1Delta mutation in Candida glabrata result in deficiency of mitochondrial phosphatidylglycerolphosphate synthase and lack of two anionic phospholipids, phosphatidylglycerol and cardiolipin. DNA sequence analysis of the PCR-amplified pel1 mutant allele revealed that the pel1 mutation resulted from a single amino-acid substitution (Glu(463)Lys) in the C-terminal part of encoded enzyme. The CgPGS1 gene cloned in a centromeric pFL38 vector functionally complemented the pel1 mutation in S. cerevisiae. Likewise, the ScPGS1 gene cloned in pCgACU5 plasmid fully complemented the Cgpgs1Delta mutation in C. glabrata. This mutation increased the cell surface hydrophobicity and decreased biofilm formation. These results support a close evolutionary relatedness of S. cerevisiae and C. glabrata and point to the relationship between expression of virulence factors and anionic phospholipid deficiency in pathogenic C. glabrata.

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Bioadhesive, non-drug-loaded nanoparticles as modulators of candidal adherence to buccal epithelial cells: a potentially novel prophylaxis for candidosis

Cited by 32 publications

References 34 publications

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Development, characterization, and in vivo assessment of mucoadhesive nanoparticles containing fluconazole for the local treatment of oral candidiasis

Sequential Exposure to Carbon Nanotubes and Bacteria Enhances Pulmonary Inflammation and Infectivity

Molecular and phenotypic analysis of mutations causing anionic phospholipid deficiency in closely related yeast species

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