Bioanalysis of antitubercular drugs using liquid chromatography

Raju, K. Kanaka; Gundeti, Manoj; Malik, Monika; Kadian, Naveen; Rashid, Mamunur; Taneja, Isha; Singh, Sheelendra Pratap; Wahajuddin, Muhammad

doi:10.1016/j.jpba.2016.11.006

Cited by 9 publications

(7 citation statements)

References 102 publications

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“…Many analytical methods have been used to determine drug plasma concentrations. The most common are high-performance liquid chromatography coupled with ultra-violet detection (HPLC-UV), liquid chromatography coupled with mass spectrometry (LC-MS), or liquid chromatography with tandem mass spectrometry (LC-MS/MS) (18,19).…”

Section: Principles Of Therapeutic Drug Monitoring In Tb Treatmentmentioning

confidence: 99%

Benefits of Therapeutic Drug Monitoring of First Line Antituberculosis Drugs

Pršo

Žideková

Dohál

et al. 2020

Acta Medica Martiniana

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Tuberculosis is an airborne infectious disease that remains a huge global health-related issue nowadays. Despite constant approvals of newly developed drugs, the use of first-line antituberculosis medicines seems reasonable in drug-susceptible Mycobacterium tuberculosis strains. Therapeutic drug monitoring presents a useful technique for the determination of plasma drug concentration to adjust appropriate dose regimes. In tuberculosis treatment, therapeutic drug monitoring is aiding clinicians in selecting an optimal therapeutic level, which is essential for the personalisation of therapy. This review is aimed at clarifying the use of therapeutic drug monitoring of the first-line antituberculosis drugs in routine clinical practice.

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Section: Principles Of Therapeutic Drug Monitoring In Tb Treatmentmentioning

confidence: 99%

Benefits of Therapeutic Drug Monitoring of First Line Antituberculosis Drugs

Pršo

Žideková

Dohál

et al. 2020

Acta Medica Martiniana

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“…Streptomycin (Figure ), the first antibiotic widely used for the treatment of TB, exerted an inhibitory effect by preventing the initiation of protein translation (Chetty, Ramesh, Singhpillay, & Soliman, ). Isoniazid and ethionamide interfered with the biosynthesis of mycolic acids found in cell walls by inhibiting the NADH‐dependent enoyl‐acyl carrier protein reductase (Raju et al, ). Rifampicin had been classified as a lipophilic ansamycin, which targeted the β subunit of RNA polymerase and inhibited the synthesis of RNA.…”

Section: Introductionmentioning

confidence: 99%

Opportunities and challenges of using five‐membered ring compounds as promising antitubercular agents

Wang

et al. 2020

Drug Development Research

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Tuberculosis (TB), a chronic infectious disease, is one of the greatest risks to human beings and 10 million people were diagnosed with TB and 1.6 million died from this disease in 2017. In addition, with the emergence of multidrug-resistant tuberculosis (MDR-TB) and extensively drug-resistant tuberculosis (XDR-TB), the TB situation has become even worse, which has aggravated the mortality and spread of this disease.To overcome this problem, research into novel antituberculosis agents with enhanced activities against MDR-TB, reduced toxicity, and shortened duration of therapy is of great importance. Fortunately, many novel potential anti-TB drug candidates with five-membered rings, which are most likely to be effective against sensitive and resistant strains, have recently entered clinical trials. Different five-membered rings such as furans, pyranoses, thiazoles, pyrazolines, imidazoles, oxazolidinone, thiazolidins, isoxazoles, triazoles, oxadiazoles, thiadiazoles, and tetrazoles have been designed, prepared, and evaluated for their antimycobacterial activity against Mycobacterium tuberculosis. In this article, we highlight the recent advances made in the discovery of novel five-membered ring compounds and focus on their antitubercular activities, toxicity, structure-activity relationships, and mechanisms of action. K E Y W O R D Santi-TB drug, antitubercular activities, five-membered ring, MDR-TB, tuberculosis

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“…Numerous HPLC methods are reported in the literature for the analysis of INH, AcINH, PZA, and RMP in biological fluids [11,12]; however, limitations of these protocols include complex extraction methods, the use of two analytical runs [10,13], and a maximum number of three detected analytes [13], among other aspects. Most of the chromatographic methods include the spectrophotometric detection of the eluted compounds.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the correct monitoring of plasma levels of INH, AcINH, PZA, RMP, and EMB is essential for the therapeutic success and safety of the treatment [4,10]. Several analytical methods have been reported to quantify the first-line antituberculosis (anti-TB) drugs [11,12]; however, due to the different physicochemical properties between these drugs, it has been difficult to develop a technique that simultaneously quantifies all the compounds of interest from a single analysis procedure [4,13]. LC techniques have been widely used for drug concentration analysis because of their selectivity, specificity, and sensitivity; this latter factor is variable depending on the detection equipment used as it directly influences the cost of the analysis.…”

Section: Introductionmentioning

confidence: 99%

“…Numerous HPLC methods are reported in the literature for the analysis of INH, AcINH, PZA, and RMP in biological fluids [11,12]; however, limitations of these protocols Article Related Abbreviations: AcINH, acetyl-isoniazid; anti-TB, antituberculosis; AUCo-∞, area under time-concentration curve from zero to infinity; C max , maximum plasma concentration; CV%, coefficient of variation; EMB, ethambutol; INH, isoniazid; LLOQ, lower LOQ; PZA, pyrazinamide; RE%, relative error; REC%, Recovery; RMP, rifampicin; t 1/2 , elimination half-life; TB, tuberculosis; T max , time maximum plasma concentration; UV/VIS, ultraviolet/visible; V d , volume of distribution include complex extraction methods, the use of two analytical runs [10,13], and a maximum number of three detected analytes [13], among other aspects. Most of the chromatographic methods include the spectrophotometric detection of the eluted compounds.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of pyrazinamide, isoniazid, acetyl‐isoniazid, and rifampicin by a high‐performance liquid chromatography method in human plasma from patients with tuberculosis

Hernández-González

Zarazúa

Veytia-Bucheli

et al. 2020

J of Separation Science

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The aim of this study was to establish and validate an alternative high‐performance liquid chromatography method for simultaneous quantification of pyrazinamide, isoniazid, acetyl‐isoniazid and rifampicin in plasma of patients under treatment for tuberculosis. The performed method was lineal (r2 > 0.99) in the range of 2.00–50.00 μg/mL for pyrazinamide, 0.50–20.00 μg/mL for both acetyl‐isoniazid and isoniazid, and 1.20–25.00 μg/mL for rifampicin. Precision and trueness were demonstrated with coefficient of variation < 15% and deviations < 15%, respectively, for quality controls samples. The lower limits of quantification were 2.00, 0.50, 0.50, and 1.20 μg/mL for pyrazinamide, isoniazid, acetyl‐isoniazid and rifampicin, respectively. The method was applied for the analysis of plasma from patients with tuberculosis. This method allowed ensuring reliable quantification of the target compounds and their pharmacokinetics parameters. In general, the mean values of maximum concentration of each antituberculosis drug were located within their respective reference therapeutic ranges. However, patients with sub‐therapeutic plasma concentrations of isoniazid and rifampicin were detected. This is the first analytical technique that simultaneously quantifies isoniazid, acetyl‐isoniazid, rifampicin, and pyrazinamide concentrations from plasma samples by high‐performance liquid chromatography with ultraviolet/visible. The proposed method could be applied for therapeutic drug monitoring and pharmacokinetics studies of the four compounds throughout the treatment of tuberculosis patients.

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Bioanalysis of antitubercular drugs using liquid chromatography

Cited by 9 publications

References 102 publications

Benefits of Therapeutic Drug Monitoring of First Line Antituberculosis Drugs

Benefits of Therapeutic Drug Monitoring of First Line Antituberculosis Drugs

Opportunities and challenges of using five‐membered ring compounds as promising antitubercular agents

Quantification of pyrazinamide, isoniazid, acetyl‐isoniazid, and rifampicin by a high‐performance liquid chromatography method in human plasma from patients with tuberculosis

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