Bioanalysis of β2-agonists by hyphenated chromatographic and mass spectrometric techniques

Polettini, Aldo

doi:10.1016/s0378-4347(96)00015-1

Cited by 66 publications

(45 citation statements)

References 39 publications

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“…Among them, chloro(chloromethyl)-dimethylsilane 13 produces a dimethylsilamorpholine (DMS) derivative with five ions [i.e., 331 (M-CH 3 ) and 289 (Mt Bu) m/z, Figure 1b] with a relative intensity of ≥10% of the base peak that confirms the presence of clenbuterol. 14 …”

Section: Introductionmentioning

confidence: 99%

A straightforward route to obtain 13C1-labeled clenbuterol

González-Antuña¹,

Lavandera²,

Rodríguez-González³

et al. 2011

Tetrahedron

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Section: Introductionmentioning

confidence: 99%

A straightforward route to obtain 13C1-labeled clenbuterol

González-Antuña¹,

Lavandera²,

Rodríguez-González³

et al. 2011

Tetrahedron

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“…Various analytical methods have been reported for detecting clenbuterol in animal tissues or urine (Polettini, 1996;Mitchell and Dunnavan, 1998). The method currently used by most laboratories is gas chromatography-mass spectrometry (GC-MS) (Ramos et al, 2003) and high performance liquid chromatography (HPLC).…”

Section: Introductionmentioning

confidence: 99%

Comparison of time-resolved fluoroimmunoassay for clenbuterol residues in pig liver with enzyme-linked immunosorbent assay

Wang¹,

Li²,

Huo³

et al. 2007

J. Anim. Feed Sci.

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A time-resolved fl uoroimmunoassay (TR-FIA) for determination of clenbuterol (CLB) in pig liver was developed. The limit of detection (LOD) was 0.02 ng g -1 and limit of quantifi cation (LOQ), 0.08 ng g -1 . Recoveries ranged from 89.3 to 117.9% for pig liver at spiked levels of 0.1-5 ng g -1 . The results obtained by the TR-FIA and enzyme-linked immunosorbent assay (ELISA) showed a good correlation. The established TR-FIA was applied for screening pig liver from the local market and confi rmed by gas chromatography-mass spectrometry (GC-MS). This proposed technique could be used for routine screening for drug residues.

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“…Belonging to the family of b-agonists, these compounds are used for the treatment of pulmonary diseases. Their bronchodilatory effect on smooth muscle is the main pharmacological action responsible for alleviating asthmatic attacks brought about by infection or allergies, as well as treating other respiratory disorders (Ziment, 1995;Polettini, 1996). In addition to the smooth muscle relaxation effect, clenbuterol is used in agriculture to increase the lean muscle to fat ratio of livestock and is abused as a performance-enhancing agent in sport (Hanrahan, 1987;Chan and Petruzelka, 1995;Clarkson and Thompson, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…Accordingly, its determination is equally important in pharmacokinetic studies in vivo and for the clinical monitoring of patients receiving clenbuterol, since sideeffects from b-agonists, such as arrythmias and loss of breath, have been reported (Ziment, 1995). Many quantitative methods for the detection of clenbuterol in biological matrices are found in the literature, and a review by Polettini (1996) discusses this subject. These studies focus on analyzing the racemic drug; however, a racemic drug consists of a 1:1 mixture of two enantiomeric forms.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of clenbuterol enantiomers in human plasma by high-performance liquid chromatography using the macrocyclic antibiotic chiral stationary phase teicoplanin1

Aboul‐Enein

Serignese

1999

Biomed. Chromatogr.

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We report a method for the high‐performance liquid chromatographic (HPLC) chiral separation of racemic clenbuterol in human plasma. Human plasma was spiked with stock solutions of clenbuterol hydrochloride and practolol as the internal standard. Following a liquid–liquid extraction procedure with 10% (+/−)‐2‐butanol/isopropyl ether under alkaline conditions, the dried samples were reconstituted in methanol and chromatographed using a macrocyclic antibiotic chiral stationary phase (CSP) known as Chirobiotic T™ (teicoplanin). The mobile phase composition was methanol:acetonitrile (70:30, v/v), containing 0.3% (v/v) acetic acid and 0.2% (v/v) triethylamine. The resulting chromatogram achieved baseline separation for the clenbuterol enantiomers. Calibration curves (peak area ratio vs plasma concentration, n = 10) were constructed for the (−)‐R‐and (+)‐S‐clenbuterol enantiomers with a plasma concentration range of 0.25–10 µM. The correlation coefficient (r) range was 0.99988–0.99999 (mean = 0.99999). The lowest concentration measured was 0.25 µM. Inter‐ and intra‐assay variation was determined for the lowest, medium and highest plasma concentration (0.25, 2 and 10 µM) by calculating the analytical recoveries with a range of 96–104%. The percentage recoveries for the clenbuterol enantiomers were 88.4–102% over the concentration range used. Detailed methodology is presented. Copyright © 1999 John Wiley & Sons, Ltd.

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Bioanalysis of β2-agonists by hyphenated chromatographic and mass spectrometric techniques

Cited by 66 publications

References 39 publications

A straightforward route to obtain 13C1-labeled clenbuterol

A straightforward route to obtain 13C1-labeled clenbuterol

Comparison of time-resolved fluoroimmunoassay for clenbuterol residues in pig liver with enzyme-linked immunosorbent assay

Quantitative determination of clenbuterol enantiomers in human plasma by high-performance liquid chromatography using the macrocyclic antibiotic chiral stationary phase teicoplanin1

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