Bioanalytical Applications of Capillary Electrophoresis with Laser-Induced Native Fluorescence Detection

Tseng, Hua-Ming; Li, Yin; Barrett, David A.

doi:10.4155/bio.10.72

Cited by 21 publications

(8 citation statements)

References 90 publications

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“…Introduction Photometric and fluorimetric optical detection methods are frequently used in capillary based separation techniques including capillary electrophoresis (CE) and capillary liquid chromatography (cap-LC) [1][2][3][4][5][6]. While photometric detection is generally valuable and the most common detection in CE and cap-LC, the combination of the most sensitive detection method of laser-induced fluorescence (LIF) detection with CE provides a powerful separation platform with a wide range of advantages including speed, high resolution, efficiency, and sensitivity, as well as low sample and reagent consumption [7][8][9], for applications such as glycomics [10][11][12].…”

Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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Graphical Abstract (please choose)A new generation of integrated miniaturized fibre-coupled solid-state light sources based on microfabricated light emitting diode micro-array (µ-LED), micropackaged with a custom band-pass optical interference filter deposited at the end of an optical fibre, is presented and in this work and demonstrated as excitation light source for fluorescence detection in capillary electrophoresis. ALTERNATIVE IMAGES: Key words:Micro-light emitting diode array; light source; optical fibre; capillary electrophoresis; fluorescence detection; separations. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 *Manuscript (including figures, tables, text graphics and associated captions) Click here to view linked References

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Section: ) Australian Centre For Research On Separation Science and mentioning

confidence: 99%

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Vaculovičová

Akther

Maaskant

et al. 2015

Analytica Chimica Acta

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“…As well as the intrinsic low detection sensitivity drawbacks of CE, which are the limited light absorption dimension and the restricted sample injection length result in small sample injection volume, greatly restrain the application of CE‐UV to DNA analysis. Consequently, fluorescence detection has been used in DNA analysis to overcome the low sensitivity of CE (Tseng and others ; Ban and Song ; de Kort and others ; Ruan and others ). However, this solution demands labeling samples with a fluorescent marker or intercalating dyes (Hernández‐Chávez and others ; Valdes and others ).…”

Section: Limitations Advantages and Future Development Of Ce‐based mentioning

confidence: 99%

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Lian

Zeng

2017

Comp Rev Food Sci Food Safe

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Food safety and food production are closely related to the health of consumers. Food-related accidents often cause tremendous losses of personnel and property. Thus, rapid detection and analysis of ingredients in food, tracing food sources, studying the optimal conditions for food production, and more are vital for preventing incidents related to safety. Conventional analysis based on proteomics, microbial cultures, and morphology, as well as biochemical tests based on metabonomics, are considered gold standards and used frequently, but they are labor-intensive, time-consuming, tedious, error-prone, and incapable of meeting the demand for rapid and precise detection at a large scale. Alternative detection methods that utilize capillary electrophoresis have the advantages of high efficiency, high throughput, high speed, and automation; these methods are coupled with various nucleic acid detection strategies to overcome the drawbacks of traditional identification methods, and to prevent false results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic acid detection in food analysis and provides an introduction to the limitations, advantages, and future developments of this approach.

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“…According to the limited scope of the present review, a wide body of literature regarding applications of CE to pharmaceutical and biomedical analysis has not been included herewith; however, the reader can easily draw information from some excellent, recent reviews 23–26.…”

Section: Illicit and Abused Drugsmentioning

confidence: 99%

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

2011

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The present article reviews and comments the applications of capillary electrophoresis in the different areas of forensic sciences covering the time from the second half of 2009 until the first part of 2011, being the latest update of previous reviews covering the years from 2001 to 2009. Numerous articles reporting applications of capillary electrophoresis to analytical problems of potential interest for the forensic researchers and scientists can be found in the most qualified journals of analytical chemistry, analytical biochemistry, pharmacology, toxicology, laboratory medicine, human genetics, etc. However, the present review has been focused on discussing only the most relevant examples of analytical applications of capillary electrophoretic and electrokinetic techniques published in the following fields: (i) illicit and abused drugs, (ii) ions and small molecules of forensic interest, (iii) proteins and peptides of forensic interest, (iv) dyes and inks, (v) forensic DNA. The present review collects and comments on 60 references.

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Bioanalytical Applications of Capillary Electrophoresis with Laser-Induced Native Fluorescence Detection

Cited by 21 publications

References 90 publications

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Fibre coupled micro-light emitting diode array light source with integrated band-pass filter for fluorescence detection in miniaturised analytical systems

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Recent advances in the application of CE to forensic sciences, an update over years 2009–2011

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