Bioanalytical Chemistry

Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles

“…Bioassays such as immunoassays and DNA hybridisation assays are widely used in bioanalytical chemistry [1]. Immunoassays rely on the high specificity of the molecular binding between antibody and antigen and are often utilised for medical diagnosis such as pregnancy tests, or analysis of disease markers including cancer, stroke, heart attack and HIV.…”

Protein adsorption onto nanomaterials for the development of biosensors and analytical devices: A review

“…The biorecognition element for biosensors is not only limited to enzymes and antibodies but can also span a vast array of compounds including DNA, cells, microorganisms, organelles, and plant or animal tissues[4]. Point-of-care monitoring, short analyses times, easy sample preparation, and cost are currently of the upmost importance in diagnostic testing.…”

“…As it will be later specifically addressed, the rate and strengths of the initial physical interactions between proteins and surfaces dictate (to a large degree) the final conformation, stability, and activity of such proteins. This issue, that plays a major role in determining the biocompatibility of materials[2, 3], can also dictate the analytical performance of almost every analytical device that uses a biorecognition element (antigen, antibody, enzyme, nucleic acids, or even whole cells)[4]. The topic has become even more relevant in the last decade because an increasing number of applications of biosensors and other protein-based analytical devices have been presented, spanning across a wide array of applications including healthcare, security, environmental, agriculture, food control, process control, and microbiology[5, 6].…”

Synthesis of Circular Double-Stranded DNA Having Single-Stranded Recognition Sequence as molecular-Physical Probe for Nucleic Acid Hybridization Detection Based on Atomic Force microscopy Imaging

“…Fluorophores are the best-established materials, as manifested in, e.g., the Sanger method of DNA sequencing and in the high-throughput gene analysis applications using DNA chips/microarrays. 1,2 Researchers motivated by recent nanotechnology developments, on the other hand, have examined various nanoparticle tags including gold, [3][4][5] silver, 6 quantum dots, 7,8 and polymers 9 in a combined use of a variety of chemical or physical measurements. Among them, for visualization measurement/detection purposes, atomic force microscopy (AFM) studies should be interesting.…”