Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles

Bronzeau, Sandrine; Pamme, Nicole

doi:10.1016/j.aca.2007.11.035

Cited by 68 publications

(70 citation statements)

References 28 publications

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“…static bead solution in the microchannel), (f) the particle-fluid or inter-particle interaction are not considered. This numerical model has been previously validated showing a good correlation with published results [39,49].…”

Section: Numerical Model and Assumptionssupporting

confidence: 65%

Magnetic track array for efficient bead capture in microchannels

et al. 2009

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Magnetism-based microsystems, as those dedicated to immunoaffinity separations or (bio)chemical reactions, take benefit of the large surface area-to-volume ratio provided by the immobilized magnetic beads, thus increasing the sensitivity of the analysis. As the sensitivity is directly linked to the efficiency of the magnetic bead capture, this paper presents a simple method to enhance the capture in a microchannel. Considering a microchannel surrounded by two rectangular permanent magnets of different length (L m =2, 5, 10 mm) placed in attraction, it is shown that the amount of trapped beads is limited by the magnetic forces mainly located at the magnet edges. To overcome this limitation, a polyethylene terephthalate (PET) microchip with an integrated magnetic track array has been prototyped by laser photo-ablation. The magnetic force is therefore distributed all along the magnet length. It results in a multi-plug bead capture, observed by microscope imaging, with a magnetic force value locally enhanced. The relative amount of beads, and so the specific binding surface for further immunoassays, presents a significant increase of 300% for the largest magnets. The influence of the track geometry and relative permeability on the magnetic force was studied by numerical simulations, for the microchip operating with 2-mm-long magnets.

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Section: Numerical Model and Assumptionssupporting

confidence: 65%

Magnetic track array for efficient bead capture in microchannels

et al. 2009

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“…The maximum particle velocity in a weak oscillating magnetic field can be calculated by equalizing the magnetic actuation force (Eq. 24) [36] to the hydrodynamic drag force (Eq. 25): = .…”

Section: Insert Figures 6 and 7 Herementioning

confidence: 99%

Magnetic actuation of catalytic microparticles for the enhancement of mass transfer rate in a flow reactor

Lisk

Bonnot

Rahman

et al. 2016

Chemical Engineering Journal

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Permanent WRAP URL:http://wrap.warwick.ac.uk/81857 Copyright and reuse:The Warwick Research Archive Portal (WRAP) makes this work by researchers of the University of Warwick available open access under the following conditions. Copyright © and all moral rights to the version of the paper presented here belong to the individual author(s) and/or other copyright owners. To the extent reasonable and practicable the material made available in WRAP has been checked for eligibility before being made available.Copies of full items can be used for personal research or study, educational, or not-for-profit purposes without prior permission or charge. Provided that the authors, title and full bibliographic details are credited, a hyperlink and/or URL is given for the original metadata page and the content is not changed in any way. A note on versions:The version presented here may differ from the published version or, version of record, if you wish to cite this item you are advised to consult the publisher's version. Please see the 'permanent WRAP URL' above for details on accessing the published version and note that access may require a subscription. AbstractThe effect of periodic changes in particle velocity on mass transfer to the reacting surface of a magnetic particle with a diameter 225 m in laminar flow has been investigated in a microfluidic reactor. The periodic particle motion in a fluid was investigated under a sinusoidal magnetic field generated by a quadrupole arrangement of electromagnets around the reactor. The effect of operating frequency of the rotating magnetic field, intensity of the magnetic field, and phase shift between the two sets of magnets on particle dynamics has been studied. Three particle motion modes have been observed depending on the frequency of the applied field. The mass transfer rate was estimated under steady velocity and variable velocity of the particle using a mass transfer correlation by Feng and Michaelides (Int. J. Heat Mass Transfer 44 (2001) 4445). The validity of this correlation for the case of variable particle velocity has been confirmed with a 2D numerical model, describing actual hydrodynamics and mass transfer towards the particle surface. The mass transfer coefficient *Revised Manuscript (clean for typesetting) Click here to view linked References 2 depends both on the mean particle velocity and the deviation of velocity from the mean value.The periodic movement with variable particle velocity reduces the mass transfer coefficient by 7.6% as compared to steady state motion with the same mean velocity.

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“…To meet these needs, microfluidic immunomagnetic assays that use droplets to encapsulate both functionalized magnetic particles and reagents in a single microenvironment have been proposed. [33][34][35][36][37] Such droplet microfluidic assays are advantageous in their capacity to maintain a stable microenvironment within the droplet, minimize surface adsorption of target biomarker onto the channel walls, and prevent diffusioninduced spreading of contaminants and fluctuations in reagent concentration. Yet, the lack of reconfigurability, due to the channel-based continuous flow format, and issues of channel clogging remain as obstacles.…”

Section: Microfluidicsmentioning

confidence: 99%

Immunomagnetic nanoparticle-based assays for detection of biomarkers

Park¹,

Hwang²,

Lee³

2013

IJN

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The emergence of biomarkers as key players in the paradigm shift towards preventative medicine underscores the need for their detection and quantification. Advances made in the field of nanotechnology have played a crucial role in achieving these needs, and have contributed to recent advances in the field of medicine. Nanoparticle-based immunomagnetic assays, in particular, offer numerous advantages that utilize the unique physical properties of magnetic nanoparticles. In this review, we focus on recent developments and trends with regards to immunomagnetic assays used for detection of biomarkers. The various immunomagnetic assays are categorized into the following: particle-based multiplexing, signal control, microfluidics, microarray, and automation. Herein, we analyze each category and discuss their advantages and disadvantages.

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Simultaneous bioassays in a microfluidic channel on plugs of different magnetic particles

Cited by 68 publications

References 28 publications

Magnetic track array for efficient bead capture in microchannels

Magnetic track array for efficient bead capture in microchannels

Magnetic actuation of catalytic microparticles for the enhancement of mass transfer rate in a flow reactor

Immunomagnetic nanoparticle-based assays for detection of biomarkers

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