Bioanalytical method for quantification of Solifenacin in rat plasma by LC-MS/MS and its application to pharmacokinetic study

Puttagunta, Srinivasa Babu; Shaik, Rihana Parveen; Bannoth, Chandrasekhar Kothapalli; Challa, B.R.; Awen, Bahlul Z.

doi:10.1186/s40543-014-0035-0

Cited by 19 publications

(5 citation statements)

References 17 publications

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“…The peak area ratio against maralixibat concentration was used to construct a calibration graph [17][18][19]. The findings were discovered to be linear across the 5.00-100.00 ng/ml concentration levels.…”

Section: Linearitymentioning

confidence: 99%

Bioanalytical Method Development and Validation of Maralixibat in Rat Plasma by Lc-MS/MS Detection and Its Application to a Pharmacokinetic Study

2023

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Objective: To quantify maralixibat in rat plasma utilizing liquid-liquid extraction (LLE) approach, a practical, efficient, and accurate LC-MS/MS approach was devised. Methods: As an internal standard (IS), Elobixibat was adopted. Utilizing an Agilent eclipse C18, 150 mm x 4.6 mm, 3.5 µm column, the drug separation was accomplished using an isocratic mobile phase entailing acetonitrile (ACN) and buffer (1 ml Tri fluoro acetic acid into 1liter water and stir well. Filtered through 0.22µ membrane filter paper) composition of 70:30 (v/v), dispensed at 1.0 ml/min. Results: Multiple reaction monitoring (MRM) positive mode allowed for the simultaneous detection of maralixibat and elobixibat exhibiting proton adducts around m/z 676.0278-290.3625 and m/z 696.8541-480.6328, correspondingly. The correlation coefficient (r2) of the approach was ≥0.99977 across a linearity concentration spanning between 5.00–100.00 ng/ml. This technique achieved intra-day accuracy and precision between 99.31-100.93% and 0.22-6.55%, correspondingly. Across 3 freeze-thaw sessions, bench top testings, and postoperative stability investigations, maralixibat was shown to be stable. Conclusion: Through intravenous injection, this approach was effectively utilized in rats for studying the drug's pharmacokinetics.

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Section: Linearitymentioning

confidence: 99%

Bioanalytical Method Development and Validation of Maralixibat in Rat Plasma by Lc-MS/MS Detection and Its Application to a Pharmacokinetic Study

2023

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“…Therapeutic indication of this brand is to treat moderate to severe storage symptoms like urgency, increased micturition frequency and voiding symptoms associated with benign prostatic hyperplasia (BPH) in men who are not adequately responding to treatment with monotherapy [3] . Literature survey reveals spectrophotometric methods [4][5][6][7][8] , High Performance Liquid Chromatography (HPLC) [9][10][11] and Liquid Chromatography-Electrospray Ionization-Mass Spectrometry (LC-ESI-MS/MS) [11][12] methods were available for tamsulosin hydrochloride and Ultraviolet [13][14] , HPLC [15][16] , Ultra-Performance Liquid Chromatography (UPLC) [17] , high-performance thin layer chromatography (HPTLC) [18] and Liquid Chromatography with tandem mass spectrometry (LC-MS/MS) [19][20] methods were available for estimation of solifinacin succinate. Only few reverse phase high performance liquid chromatography (RP-HPLC) [21][22] method and spectrofluorimetric [23] methods were reported for the estimation of solifinacin succinate & tamsulosin hydrochloride in combination dosage forms.…”

Section: Chemically Tamsulosin Hydrochloride Is 5-[(2r)-2-{[2-(2-ethoxyphenoxy)ethyl]mentioning

confidence: 99%

Simultaneous Estimation of Solifenacin Succinate and Tamsulosin Hydrochloride in Combined Dosage Form by Using First Order Derivative Spectrophotometric Method

Yarraguntla¹,

Kumar²,

Ponnam³

et al. 2021

ijps

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Srinivasarao et al.: Derivative Spectrophotometric Method for Solifenacin Succinate and Tamsulosin HydrochlorideUsing first-order derivative techniques, a simple accurate and precise Ultraviolet spectrophotometric method was developed for the determination of solifenacin succinate and tamsulosin hydrochloride in combined dosage form. Every solifinacin succinate and tamsulosin hydrochloride was scanned in the wavelength region of 200-400 nm for determination of sampling wavelengths and selected sampling wavelengths were selected. Sampling wavelengths were chosen at 265 nm (zero crossing of solifinacin succinate) where the absorbance of tamsulosin hydrochloride was important and at 250 nm (zero crossing of tamsulosin hydrochloride) where the absorbance of solifinacin succinate was significant. For solifenacin succinate, linearity ranged from 10 to 100 μg/ml with a correlation coefficient of 0.998 and 2 to 10 μg/ml for the correlation coefficient of tamsulosin hydrochloride is 0.9988. The median recovery was considered to be satisfactory. For the routine study of solifenacin succinate and tamsulosin hydrochloride in combined dosage forms, the approach proposed can be applied.

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“…Literature survey revealed that few chromatographic methods for SOLS [6][7][8][9][10][11][12][13][14][15][16][17], FLXHC [18][19][20][21] and TOLT [22][23][24][25][26][27][28][29], spectrophotometric methods for SOLS [30,31], FLXHC [32,33] and TOLT [34][35][36], potentiometric method Equivalent weights of 10 mg of SOLS, 20 mg of FLXHC and 20 mg of TOLT were transferred into three 100 mL clean dry volumetric flasks containing 70 mL of mobile phase and the mixture was sonicated to dissolve the powder. The volume was made up to the mark with the same solvent.…”

Section: Preparation Of Pharmaceutical Dosage Forms Solutionsmentioning

confidence: 99%

Liquid Chromatographic Determination of Solifenacin Succinate, Flavoxate Hydrochloride and Tolterodine Tartrate in Bulk Drugs and Their Pharmaceutical Dosage Forms

Attia

Frag

Ahmed

2016

J. Chil. Chem. Soc.

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A simple, precise, specific and accurate reversed phase HPLC (RP-HPLC) method has been developed for the determination of solifenacin succinate (SOLS), flavoxate HCl (FLXHC) and toltoridine tartarate (TOLT) in bulk and pharmaceutical dosage forms. The proposed RP-HPLC method was carried out using Xterra RP-18 column (5 μm practical size, 25 cm x 4.6 mm i.d.). The flow rate, the injection volume and the detection wavelength were 1.0 mL/min, 20 mL and 200 nm, respectively. The mobile phase consisted of 0.05 M pentane sulfonic acid sodium salt (SOLS: pH 3.0±0.05, FLXHC and TOLT: pH 5.5±0.05) and acetonitrile (50:50 v/v). The retention times for SOLS, FLXHC and TOLT drugs were found to be 4.1±0.1 min, 4.3±0.0 min and 5.8±0.1 min, respectively. The calibration was linear over the concentration range of 0.1-100 μg/mL. The mean recoveries for SOLS, FLXHC and TOLT drugs were about 99.80%, 100.43% and 100.00%, respectively. The method was validated according to the ICH guidelines with respect to specificity, linearity, accuracy, precision and robustness.

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Bioanalytical method for quantification of Solifenacin in rat plasma by LC-MS/MS and its application to pharmacokinetic study

Cited by 19 publications

References 17 publications

Bioanalytical Method Development and Validation of Maralixibat in Rat Plasma by Lc-MS/MS Detection and Its Application to a Pharmacokinetic Study

Bioanalytical Method Development and Validation of Maralixibat in Rat Plasma by Lc-MS/MS Detection and Its Application to a Pharmacokinetic Study

Simultaneous Estimation of Solifenacin Succinate and Tamsulosin Hydrochloride in Combined Dosage Form by Using First Order Derivative Spectrophotometric Method

Liquid Chromatographic Determination of Solifenacin Succinate, Flavoxate Hydrochloride and Tolterodine Tartrate in Bulk Drugs and Their Pharmaceutical Dosage Forms

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