Bioapplications of DNA nanotechnology at the solid–liquid interface

Wang, Wenjing; Yu, Sha; Huang, Shan; Bi, Sai; Han, Heyou; Zhang, Jian Rong; Lu, Yi; Zhu, Jun

doi:10.1039/c8cs00402a

Cited by 80 publications

(52 citation statements)

References 246 publications

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“…Utilization of the fluid perfusion chamber permits the nano-infrared investigation on the same spot of interest under different aqueous conditions. This capability allows for studying a broad range of solid/liquid interfacial chemical and biological processes, such as heterogeneous chemical reactions and catalysis, 48 polymer membranes for biofouling mitigation, 49 DNA nanotechnology at the solid/liquid interface, 50 drug-protein interactions, 51 and functionalities of cell membranes. 52 appropriate contact resonance that provides the best signal quality.…”

Section: Discussionmentioning

confidence: 99%

Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

Wang

González-Fialkowski²,

Li³

et al. 2020

Preprint

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Atomic force microscopy-infrared microscopy (AFM-IR) provides a route to bypass Abbe’s diffraction limit through photothermal detections of infrared absorption. With the combination of total internal reflection, AFM-IR can operate in the aqueous phase. However, AFM-IR in contact mode suffers from surface damage from the lateral shear force between the tip and sample, and can only achieve 20~25-nm spatial resolution. Here, we develop the liquid-phase peak force infrared (LiPFIR) microscopy that avoids the detrimental shear force and delivers an 8-nm spatial resolution. The non-destructiveness of the LiPFIR microscopy enables <i>in situ</i> chemical measurement of heterogeneous materials and investigations on a range of chemical and physical transformations, including polymer surface reorganization, hydrogen-deuterium isotope exchange, and ethanol-induced denaturation of proteins. We also perform LiPFIR imaging of the budding site of yeast cell wall in the fluid as a demonstration of biological applications. LiPFIR unleashes the potential of in liquid AFM-IR for chemical nanoscopy.

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Section: Discussionmentioning

confidence: 99%

Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

Wang

González-Fialkowski²,

Li³

et al. 2020

Preprint

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“…System 1 SNAs with different DNA surface coverages were synthesized by incubating the thiolated oligonucleotides with 15 nm AuNPs at different ratios prior to the freeze-thaw cycle. The following ratios of thiolated DNA per cm 2 of AuNP surface area were used: (1) 6 × 10 13 , (2) 5 × 10 13 , (3) 2.5 × 10 13 . System 2 SNAs with different DNA surface coverages were obtained by displacing the bound DNA on the SNAs with thiolated PEG.…”

Section: Surface Coverage Tuningmentioning

confidence: 99%

“…In general terms, biomolecular binding and activity at the nanoparticle surface-solution interface can be significantly affected by interfacial factors including binding hindrance or enhancement, reversible adsorption, valence, orientation and curvature. [12][13][14][15] For enzymes acting on nanoparticle-bound substrates, 16 this means a transition from a bulk regime where enzyme activity is dominated by molecular colliding, to a new nano-interfacial regime where the above factors can significantly alter (and sometimes enhance) enzyme activity. [16][17][18] A curious aspect of SNAs is their interaction with nucleases, and specifically how these enzymes interact with DNA/RNA when densely displayed on a nanoparticulate scaffold (as opposed to free in solution).…”

Section: Introductionmentioning

confidence: 99%

Tuning DNA–nanoparticle conjugate properties allows modulation of nuclease activity

et al. 2021

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“…[97] This was invented by Nadrian Seeman, as a method to order protein molecules in a crystalline lattice and later on evolved with the concepts of DNA tiles and the breakthrough of DNA origami by Rothemund, [101] finally permitting to expand this technology to complex nano-to microstructures with tailored geometry, possibility to drive nanodevices and engineering artificial DNA-based machines (such as bioinspired molecular computing and robots). [102] Although the initial stages of DNA nanotechnology studies took place in aqueous example poly(amidoamide) (PAMAM) dendrimers. [103] A microintaglio printing method for RNA arraying has been optimized, by exploiting the complementarity of the DNA probes with the RNA sequences.…”

Section: Nucleic Acidsmentioning

confidence: 99%

Artificial Biosystems by Printing Biology

Arrabito

Ferrara

Bonasera

et al. 2020

Small

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The continuous progress of printing technologies over the past 20 years has fueled the development of a wide plethora of applications in materials sciences, flexible electronics and biotechnologies. More recently, printing methodologies have started up to explore the world of Artificial Biology, offering new paradigms in the direct assembly of Artificial Biosystems (small condensates, compartments, networks, tissues and organs) by mimicking the result of the evolution of living systems and also by redesigning natural biological systems, taking inspiration from them. This recent progress is reported in terms of a new field here defined as Printing Biology, resulting from the intersection between the field of printing and the bottom up Synthetic Biology. Printing Biology explores new approaches for the reconfigurable assembly of designed lifelike or life-inspired structures. This review presents this emerging field, highlighting its main features, i.e. printing methodologies (from 2D to 3D), molecular ink properties, deposition mechanisms, and finally the applications and future challenges. Printing Biology is expected to show a growing impact on the development of biotechnology and lifeinspired fabrication. Printing Biology employs different technologies dispensing molecular inks with tunable composition (molecules, polymers, biomolecules) and drop sizes (from nano-up to macroscale) onto solids or into liquids to develop lifelike or life-inspired artificial biosystems (from small condensates, to compartments up to networks, tissues and organs). This work reviews the extraordinary potential of this emerging research field.

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Bioapplications of DNA nanotechnology at the solid–liquid interface

Abstract: This review provides an insight into the bioapplications of DNA nanotechnology at the solid–liquid interfaces, including flat interfaces, nanoparticle interfaces and soft interfaces.

Cited by 80 publications

References 246 publications

Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

Liquid-Phase Peak Force Infrared Microscopy for Chemical Nano-imaging and Spectroscopy of Soft Matters

Tuning DNA–nanoparticle conjugate properties allows modulation of nuclease activity

Artificial Biosystems by Printing Biology

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