Bioassay and Radioimmunoassay of Plasma Gastrin in a Case of Zollinger-Ellison Syndrome

Abstract

Temperley. J . M . & Stagg, B. H . 1971. Bioassay and Radioimmunoassay of Plasma Gastrin in a Case of Zollinger-Ellison Syndrome. Scurid. 5. Gustrocrii.Fasting plasma gastrin levels were measured i n a patient with the Zollinger-Ellison syndrome by both bioassay and radioimmunoassay. The level remained constant follouing total gastrectomy, but fell sharply follo\iing parathyroidectomy. Although the bioassay gave gastrin levels which were consistently 2 t o 3 times higher than those obtained by radioimmunoassay. the values shelved a similar trend. H a \ ever, both methods gave almost identical results for the gastrin contents of antral mucosa and pancreatic tumour samples from the patient. It is suggested that biologically active gastrin-like substances not detectable by radioimmunoassay were present in the patient's plasma. .siry Collc~gc~ Hospiiul Mcdicul Sclrool, Lottdori, W . C. I E 655, Euglorid 6 . 735-738. Scand J Gastroenterol Downloaded from informahealthcare.com by McMaster University on 11/18/14 For personal use only. 9. Trudeau, W. L. & McGuigan, J. E. 1970. Serum gastrin levels in patients with peptic ulcer disease. Gasrrorrirrrology 59, 6-12. Scand J Gastroenterol Downloaded from informahealthcare.com by McMaster University on 11/18/14For personal use only.

Plasma gastrin and acid secretion in man following stimulation by food, meat extract, and insulin

“…It cannot be denied that other circulating peptides might react in the assay. It is known that our assay system can detect gastrin degradation products (Lewin, Hunziker, Stagg, and Wyllie, 1971) and it has also been found that some biologically active gastrin-like substances present in the plasma are not detected (Temperley and Stagg, 1971). This implies that the radioimmunoassay cannot measure absolute levels of biologically active gastrin in plasma samples.…”

The Zollinger-Ellison syndrome due to an infiltrating tumour of the stomach

“…The basal acid output of the stomach (BAO) was 17.9 m-equiv/hr and the maximal histamine response (MHR) using 0.04 mg histamine acid phosphate per kg by intravenous infusion was 23-8 m-equiv/hr giving a ratio BAO: MHR of 75%. Results of plasma gastrin assays (resting) were: radioimmunoassay (Temperley and Stagg, 1971) > 1 ng/ml (normal 80-100 pg/ml), bioassay (Smith, Lawrence, Colin-Jones, and Schild, 1970) 100 ng/ml (normal unrecordable).…”

Bioassay and Radioimmunoassay of Gastrin and Significance of Gastrin Degradation Products

“…Concentrations of gastrin in the six arterial and venous plasma samples were es timated by both bioassay [10] and radioimmunoassay [11]. Because of random vari ation in both of the assays, each sample was assayed 5-9 times according to the fol lowing plan.…”