Bioassay‐Based Isolation and Identification of Phenolics from Sweet Cherry That Promote Active Glucose Consumption by HepG2 Cells

Abstract

A variety of phenolics had been found to be functional in promoting cellular glucose consumption that is important for blood glucose regulation. Sweet cherry (Prunus avium) is rich in such kinds of phenolics, including hydrocinnamic acids, anthocyanins, flavonols, and flavan-3-ols. Furthermore, a sweet cherry phenolics-rich extract (PRE) was found to be effective in promoting HepG2 glucose consumption. Seventeen components were preliminarily identified by HPLC-ESI-MS, including 9 hydrocinnamic acids, 4 anthocyanins, 3 flavonols, and 1 flavan-3-ol. To investigate the cellular glucose consumption-promotion activity of different phneolics subclasses, the phenolics were further fractionated into an anthocyanin-rich fraction (ARF), hydrocinnamic acid-rich fraction (HRF), and flavonol-rich fraction (FRF) through liquid-liquid extraction and mix-mode cation-exchange solid-phase extraction. The 3 fractions promoted HepG2 glucose consumption to different levels, with the promotion effects of HRF and FRF stronger than that of the ARF. The results provide guidance on the use of sweet cherry as a functional fruit.

Antioxidant Status, Antidiabetic Properties and Effects on Caco-2 Cells of Colored and Non-Colored Enriched Extracts of Sweet Cherry Fruits

“…The antidiabetic properties of sweet cherries are already known [ 7 , 29 , 30 ]. Lachin [ 30 ] reported that diabetic rats fed with 200 mg of cherry extract per kg of body weight for 30 days showed reduced blood glucose and urinary microalbumin levels, proving that the ingestion of these fruits can protect pancreatic β-cells and retard glucose absorption.…”

“…Lachin [ 30 ] reported that diabetic rats fed with 200 mg of cherry extract per kg of body weight for 30 days showed reduced blood glucose and urinary microalbumin levels, proving that the ingestion of these fruits can protect pancreatic β-cells and retard glucose absorption. Besides, Cao et al [ 29 ] revealed that fractionated extracts of Black Pearl sweet cherry cultivar rich in anthocyanins, hydroxycinnamic acids, and flavonols also demonstrated antidiabetic properties by promoting cellular glucose consumption in HepG2 cells.…”

Antioxidant Status, Antidiabetic Properties and Effects on Caco-2 Cells of Coloured and Non-Coloured Enriched Extracts of Sweet Cherry Fruits

“…As referred above, it was already reported that sweet cherries have antidiabetic properties [7,29,30]. Hereupon, human colorectal adenocarcinoma Caco-2 cells were selected given two reasons: (i) they are a model of the intestinal epithelium, since, after differentiation, they form monolayers, that reproduce several characteristics of intestinal epithelial cells (e.g.…”

Resveratrol metabolites ameliorate insulin resistance in HepG2 hepatocytes by modulating IRS-1/AMPK

“…[4][5][6] Insulin resistance, a state of reduced response to insulin, is a pivotal pathological feature of T2DM and the primary organs responsive to insulin are liver, skeletal muscle and adipose tissue. 7,8 Among these tissues, 90% of the endogenous glucose production occurs in the liver, and increased hepatic gluconeogenesis seems to be the probable cause of fasting hyperglycemia, which eventually exacerbates insulin resistance. 9,10 Thus, to ameliorate hepatic insulin resistance and increase sensitivity, various synthetic pharmaceuticals have already been in the market.…”