Bioassay-guided isolation of glycoprotein SPG-56 from sweet potato Zhongshu-1 and its anti-colon cancer activity in vitro and in vivo

Wang, Meimei; Ma, Hang; Tian, Chen; Liu, Shanshan; Yang, Xiaoli; Zhou, Deqi; Li, Yanlin; Nie, Hui; Li, Xuegang

doi:10.1016/j.jff.2017.05.049

Cited by 22 publications

(7 citation statements)

References 19 publications

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“…The amino acid compositions of nodules were analyzed in an automatic amino acid analyzer (type L-8800, Hitachi Ltd., Tokyo, Japan), as described previously [ 102 ]. Briefly, about 0.1 g of fresh samples were cut off and ground in 5% 5-sulfosalicylic acid dehydrated for the extraction.…”

Section: Methodsmentioning

confidence: 99%

Genome Wide Transcriptome Analysis Reveals Complex Regulatory Mechanisms Underlying Phosphate Homeostasis in Soybean Nodules

Xue

Zhuang

Zhu

et al. 2018

IJMS

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Phosphorus (P) deficiency is a major limitation for legume crop production. Although overall adaptations of plant roots to P deficiency have been extensively studied, only fragmentary information is available in regard to root nodule responses to P deficiency. In this study, genome wide transcriptome analysis was conducted using RNA-seq analysis in soybean nodules grown under P-sufficient (500 μM KH2PO4) and P-deficient (25 μM KH2PO4) conditions to investigate molecular mechanisms underlying soybean (Glycine max) nodule adaptation to phosphate (Pi) starvation. Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity. Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively. Nodule transcript profiling revealed that a total of 2055 genes exhibited differential expression patterns between Pi sufficient and deficient conditions. A set of (differentially expressed genes) DEGs appeared to be involved in maintaining Pi homeostasis in soybean nodules, including eight Pi transporters (PTs), eight genes coding proteins containing the SYG1/PHO81/XPR1 domain (SPXs), and 16 purple acid phosphatases (PAPs). The results suggest that a complex transcriptional regulatory network participates in soybean nodule adaption to Pi starvation, most notable a Pi signaling pathway, are involved in maintaining Pi homeostasis in nodules.

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Section: Methodsmentioning

confidence: 99%

Genome Wide Transcriptome Analysis Reveals Complex Regulatory Mechanisms Underlying Phosphate Homeostasis in Soybean Nodules

Xue

Zhuang

Zhu

et al. 2018

IJMS

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“…Polyphenolics from the leaves hinder the mutation of healthy cells as well as the proliferation of cancer cells . Our previous study also revealed that glycoprotein SPG-56 induced caspase-dependent apoptosis in HCT-116 colon tumor . However, the properties of steroids from sweet potato are barely reported.…”

Section: Introductionmentioning

confidence: 95%

“…17 Our previous study also revealed that glycoprotein SPG-56 induced caspase-dependent apoptosis in HCT-116 colon tumor. 18 However, the properties of steroids from sweet potato are barely reported. In this work, we evaluated the role of daucosterol linoleate (DL) on breast cancer and elucidated the fundamental mechanism.…”

Section: ■ Introductionmentioning

confidence: 99%

Role of Daucosterol Linoleate on Breast Cancer: Studies on Apoptosis and Metastasis

Han

Liu

et al. 2018

J. Agric. Food Chem.

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The antitumor property of steroids in sweet potato ( Ipomoea batatas L.) remains poorly understood. Herein, we investigated the anticancer effect on breast carcinoma of daucosterol linoleate (DL), a steroid isolated from sweet potato. DL inhibited the cell viability of estrogen receptor (ER)-positive MCF-7 breast cancer cells at an IC value of 53.27 ± 9.02 μg/mL, while the effect was modest in ER-negative MDA-MB-231 breast cancer cells. Flow cytometry indicated that the DL-induced apoptosis in MCF-7 cells is dose-dependent. However, DL inhibited tumor growth and tumor weight at 100 mg/kg in MCF-7 xenograft nude mice. DL diminished the expression of Bcl-xl, Bcl-2, and XIAP, while increasing Bax, Bad, and activated caspase-dependent apoptosis in tumor tissues. Furthermore, DL inactivated the upstream Pi3k/Akt/NF-κB pathway. In the 4T1 spontaneous metastasis model, DL blocked metastasis progression, decreased the number of visible metastasis foci and inhibited metastasis size distribution in lung tissue. Further studies showed that DL suppressed VEGF, MMP 2, and MMP 9 expression in both tumor and lung tissues. From these results, we can assume that DL is a potential adjuvant therapy for ER-positive breast cancer patients.

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“…For the determination of the amino acid concentration in soybean nodules and seeds, about 0.1 g of fresh sample was ground with 1.5 ml of 5% 5‐sulfosalicylic acid. Then samples were centrifuged at 9643 g for 30 min at 4°C, and the supernatant was collected and used to determine the amino acid concentration through an amino acid analyzer (Hitachi L‐8800; Hitachi, Tokyo, Japan) as described previously (Wang et al., 2017).…”

Section: Methodsmentioning

confidence: 99%

Phosphate starvation responsive GmSPX5 mediates nodule growth through interaction with GmNF‐YC4 in soybean (Glycine max)

et al. 2021

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SUMMARY Phosphorus (P) deficiency adversely affects nodule development as reflected by reduced nodule fresh weight in legume plants. Though mechanisms underlying nodule adaptation to P deficiency have been studied extensively, it remains largely unknown which regulator mediates nodule adaptation to P deficiency. In this study, GUS staining and quantitative reverse transcription‐PCR analysis reveal that the SPX member GmSPX5 is preferentially expressed in soybean (Glycine max) nodules. Overexpression of GmSPX5 enhanced soybean nodule development particularly under phosphate (Pi) sufficient conditions. However, the Pi concentration was not affected in soybean tissues (i.e., leaves, roots, and nodules) of GmSPX5 overexpression or suppression lines, which distinguished it from other well‐known SPX members functioning in control of Pi homeostasis in plants. Furthermore, GmSPX5 was observed to interact with the transcription factor GmNF‐YC4 in vivo and in vitro. Overexpression of either GmSPX5 or GmNF‐YC4 significantly upregulated the expression levels of five asparagine synthetase‐related genes (i.e., GmASL2–6) in soybean nodules. Meanwhile, yeast one‐hybrid and luciferase activity assays strongly suggested that interactions of GmSPX5 and GmNF‐YC4 activate GmASL6 expression through enhancing GmNF‐YC4 binding of the GmASL6 promoter. These results not only demonstrate the GmSPX5–GmNF‐YC4–GmASL6 regulatory pathway mediating soybean nodule development, but also considerably improve our understanding of SPX functions in legume crops.

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Bioassay-guided isolation of glycoprotein SPG-56 from sweet potato Zhongshu-1 and its anti-colon cancer activity in vitro and in vivo

Cited by 22 publications

References 19 publications

Genome Wide Transcriptome Analysis Reveals Complex Regulatory Mechanisms Underlying Phosphate Homeostasis in Soybean Nodules

Genome Wide Transcriptome Analysis Reveals Complex Regulatory Mechanisms Underlying Phosphate Homeostasis in Soybean Nodules

Role of Daucosterol Linoleate on Breast Cancer: Studies on Apoptosis and Metastasis

Phosphate starvation responsive GmSPX5 mediates nodule growth through interaction with GmNF‐YC4 in soybean (Glycine max)

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