Bioassay Method for Toxicity Studies of Toxic Bait Formulations to Ceratitis capitata (Diptera: Tephritidae)

Baronio, Cléber Antonio; Bernardi, Daniel; Nunes, Marcelo Zanelato; Pasinato, Joel; García, Flávio Roberto Mello; Botton, Marcos

doi:10.1007/s13744-018-0653-0

Cited by 3 publications

(3 citation statements)

References 32 publications

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“…When the bait spray is applied on a substrate that is not harvested, such as the leaves in a fruit crop system, this can also reduce the risk of food contamination with insecticides [12]. Bait sprays have proven to be effective in controlling pests such as Ceratitis capitata, Bactrocera oleae, Diabrotica virgifera virgifera, and Drosophila suzukii [13][14][15][16][17]. In addition, the quantity of insecticide used, and the number of bait spray applications each year can be reduced [14,18,19].…”

Section: Introductionmentioning

confidence: 99%

Studying foraging behavior to improve bait sprays application to control Drosophila suzukii

Escobedo-Quevedo,

Lankheet,

Pen

et al. 2024

BMC Ecol Evo

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Background Foraging behavior in insects is optimised for locating scattered resources in a complex environment. This behavior can be exploited for use in pest control. Inhibition of feeding can protect crops whereas stimulation can increase the uptake of insecticides. For example, the success of a bait spray, depends on either contact or ingestion, and thus on the insect finding it. Methods To develop an effective bait spray against the invasive pest, Drosophila suzukii, we investigated aspects of foraging behavior that influence the likelihood that the pest interacts with the baits, in summer and winter morphotypes. We video-recorded the flies’ approach behavior towards four stimuli in a two-choice experiment on strawberry leaflets. To determine the most effective bait positioning, we also assessed where on plants the pest naturally forages, using a potted raspberry plant under natural environmental conditions. We also studied starvation resistance at 20 °C and 12 °C for both morphs. Results We found that summer morph flies spent similar time on all baits (agar, combi-protec, yeast) whereas winter morphs spent more time on yeast than the other baits. Both morphs showed a preference to feed at the top of our plant’s canopy. Colder temperatures enhanced survival under starvation conditions in both morphs, and mortality was reduced by food treatment. Conclusions These findings on feeding behavior support informed decisions on the type and placement of a bait to increase pest control.

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Section: Introductionmentioning

confidence: 99%

Studying foraging behavior to improve bait sprays application to control Drosophila suzukii

Escobedo-Quevedo,

Lankheet,

Pen

et al. 2024

BMC Ecol Evo

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“…The period of food deprivation prior to the installation of the bioassay was not mentioned in the studies (Raga & Sato 2011). However, these factors may change the susceptibility of insects over time, as verified for Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) (Barronio et al 2019). In view of the need for standardized bioassays for conducting toxicity studies and in the selection of new formulations of toxic bait for A. fraterculus, this study aimed to develop a methodology for the evaluation of toxic bait formulations on adults of A. fraterculus, using as a toxicity model the ready-to-use formulation Success™ 0.02CB.…”

Section: Introductionmentioning

confidence: 99%

A Laboratory Bioassay Method to Assess the Use of Toxic Bait on Anastrepha fraterculus (Weidemann 1830)

et al. 2019

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The lack of standardization of bioassays for the evaluation of toxic bait toxicity on the South American fruit fly, Anastrepha fraterculus (Weidemann 1830), has led to erroneous interpretations of assay results. The objective of this study was to develop a methodology for the standardization and validation of toxicological tests on A. fraterculus toxic bait using the Success™ 0.02CB formulation (80 mg L −1 of spinosad). Anastrepha fraterculus adults, obtained from larvae reared on an artificial diet, showed higher susceptibility (LT 50 = 48.96 h) than adults from larvae reared on cattley guava (LT 50 = 53.83 h) and mango fruit (LT 50 = 53.55 h). Anastrepha fraterculus adults at the age of five (LT 50 = 65.30 h), 15 (LT 50 = 59.01 h), and 30 (LT 50 = 55.53 h) days presented similar toxicity. The consumption of toxic bait (4.74 mg) increased at 15 days, a fact also observed with insects without food deprivation. In addition, the absence of a food source (artificial diet) with the toxic bait significantly reduced adult mortality time by 7 h (LT 50 = 57.42 h). In relation to exposure time, adults exposed to toxic bait for 1 h reduced consumption by 25%; however, they showed the same susceptibility as insects exposed to 2 (LT 50 = 55.72 h), 4 (LT 50 = 57.64 h), and 8 h (LT 50 = 57.76 h). However, with 24 h of food deprivation, they had a higher susceptibility (LT 50 = 46.48 h). Five-day-old A. fraterculus adults fed an artificial diet before being deprived of food for 12 or 24 h, then exposed to toxic bait for 4 h in the absence of a food source, are considered optimum conditions to evaluate the toxicity of toxic bait.

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“…When the bait spray is applied on a substrate that is not harvested, such as the leaves in a fruit crop system, this can also reduce the risk of food contamination with insecticides (Tamez-Guerra et al, 2018). Bait sprays have proven to be effective in controlling pests such as Ceratitis capitata, Bactrocera oleae, Diabrotica virgifera virgifera and Drosophila suzukii (Spencer et al, 2009;Cha et al, 2013Cha et al, , 2015Varikou et al, 2016;Baronio et al, 2019;Baker et al, 2020;Noble et al, 2023). In addition, it has been proven to reduce the quantity of insecticide used, and the number of bait spray applications each year in olive trees to control B. olea (Varikou et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Optimizing toxic bait composition and application to control Drosophila suzukii

Escobedo Quevedo

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Bioassay Method for Toxicity Studies of Toxic Bait Formulations to Ceratitis capitata (Diptera: Tephritidae)

Abstract: Keywords T o x i c i t y , m e t h o d o l o g y , s p i n o s a d , Mediterranean fruit fly

Cited by 3 publications

References 32 publications

Studying foraging behavior to improve bait sprays application to control Drosophila suzukii

Studying foraging behavior to improve bait sprays application to control Drosophila suzukii

A Laboratory Bioassay Method to Assess the Use of Toxic Bait on Anastrepha fraterculus (Weidemann 1830)

Optimizing toxic bait composition and application to control Drosophila suzukii

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