Bioassay of Components Eluted from Electric Arc Furnace Steel Slag Using Microalgae &lt;i&gt;Chlorella&lt;/i&gt;

Takahashi, Toshiyuki; Yokoyama, Seiji

doi:10.2355/tetsutohagane.tetsu-2014-130

Cited by 5 publications

(50 citation statements)

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“…These physical factors eventually cause inactive chlorophyll because of degradation [11][12][13]. In fact, a previous study using Chlorella-like algae [10] demonstrated that algae without stress irradiated only red fluorescence derived from chlorophyll (green curve in Figure 2C).…”

Section: Superfood and Functional Food -The Development Of Superfoodsmentioning

confidence: 92%

“…Moreover, this instrument presents benefits for evaluation of algal status because the excitation efficiency of the green laser at 532 nm in the yellow fluorescence is higher than that of a conventionally used blue laser at 488 nm (Figure 3) [10]. It suggests that the red and yellow fluorescence intensities are regarded respectively as indices of vigorous algae and of variant algae when the green laser irradiates algae moving through the capillary of this flow cytometer [11,13].…”

Section: Superfood and Functional Food -The Development Of Superfoodsmentioning

confidence: 99%

“…Red fluorescence is detected in the red fluorescence channel through a 680/30 nm band pass filter. Simultaneously, a yellow fluorescence channel through a 576/28 nm band pass filter is used [10][11][12][13]. Each fluorescence is converted into an electrical pulse.…”

Section: Algal Characteristics Using Fcmmentioning

confidence: 99%

“…Several environmental pollution laws, however, have restricted slag use in aquatic environments because steel slag contains environmentally hazardous substances. The toxicity of eluate from EAF slag for aquatic organisms remains poorly understood [11,13,21], but the physiochemical properties and effects of converter slag on organisms have been documented often [22][23][24][25][26][27].…”

Section: Features Of Steel Slag Used For This Studymentioning

confidence: 99%

“…This study specifically examined stainless steel slag (designated as slag A) and common steel slag (slag B), exhausted respectively from oxidation processes of stainless and common steelmaking in EAF processes [11,13]. Table 1 presents compositions of EAF slags used for this study [11,13,21,28,29].…”

Section: Features Of Steel Slag Used For This Studymentioning

confidence: 99%

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Quality Assessment of Microalgae Exposed to Trace Metals Using Flow Cytometry

Takahashi¹

2017

Superfood and Functional Food - The Development of Superfoods and Their Roles as Medicine

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Seaweed has long been an important kitchen ingredient and a functional food material. Microalgae have attracted the same attention as seaweed from food, pharmaceutical and cosmetic companies because several algae contain unique functional materials. Industry application of algae requires the selection of useful algal species, evaluation of their features and monitoring of their quality in culture. Taking Chlorella for example, this chapter presents a method using flow cytometry (FCM) to assess not only the number of algae but also algal quality. First, Chlorella was cultured in media containing eluate from steel slag as an experimental factor and trace metals. After the treatment of algae with eluate, the number and physiological features of algae were evaluated, respectively, using hemocytometry and FCM. Results show that eluate from slag induced neither lethality nor growth inhibition. Coupled with hemocytometry, FCM was used to estimate vigorous and aberrant algal status. Consequently, the eluate did not give rise to algae stresses. Interestingly, the addition of slag eluate increased the amounts of the carbonate species. The increase in the carbonate species actually triggered the potential increase in aqueous CO 2 for photosynthesis, eventually inducing algal proliferation. These analyses can support evaluation of algal features and maintenance of their quality for industry application.

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