Bioassays as one of the Green Chemistry tools for assessing environmental quality: A review

Wieczerzak, Monika; Namieśnik, Jacek; Kudłak, Błażej

doi:10.1016/j.envint.2016.05.017

Cited by 84 publications

(33 citation statements)

References 106 publications

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“…They are usually expensive, with using a large number of toxic and environmentally harmful solvents. These solvents consume a lot of resources and produce harmful and toxic wastes [6]. Therefore, it is necessary to develop a reliable technology that can meet the requirements of green analytical chemistry, and the microbial inhibition method has the advantages of green environmental protection, low cost, simple and fast operation, and multifunctional detection combining analytical concentration and toxicity.…”

Section: Instructionsmentioning

confidence: 99%

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Gong

Yang

et al. 2020

Preprint

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A whole-cell biosensor developed with yeast Saccharomyces cerevisia to detect the toxicity of chlorothalonil has been developed. This biosensor relied on the inhibition effect for metabolism by toxicants to provide detection and the degree of toxicity to yeast cells. In order to further improve the toxic sensitivity of yeast cells biosensor, the effect of the action time, the initial pH value of the medium and the temperature on inhibiting cell growth were investigated. 10Response surface regression analysis was conducted to obtain optimal culture conditions. Th effects of treated yeast morphology, ROS, DNA, caspase 3 activity were analyzed. This optimized yeast as a biosensor was used to detect chlorothalonil and heavy metals. The results are as follows: at optimal culture conditions, EC50 values of chlorothalonil to yeast biosensor determined at incubation time 4 h increased from 0.25 µg·mL -1 in the control to 0.006 µg·mL -1 , which increased 15 by 41.67 times. Compared with the control yeast cells, the morphology of optimized yeast cells were more transparent, with significantly increased intracellular vesicle rate and cell membrane permeability, intracelluar ROS increased siginificantly, DNA bands extracted was ladder, and caspase 3 activity was stimulated. The yeast biosensor had a high sensitivity to heavy metals. After analysis, many treated cells were apoptosis which was the main reason for the increasing 20 sensitivity to detect harmful substances. It was found that the method provides a new idea for the detection of harmful substances in the environment.

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Section: Instructionsmentioning

confidence: 99%

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Gong

Yang

et al. 2020

Preprint

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“…Contrastingly, toxicity bioassays play a relevant and crucial role in assessing the actual or potential impacts of chemical pollutants on the environment and human health [36]. Toxicity bioassays have the advantage of integrating the effects of all chemical compounds (including unknown substances, some of which may be breakdown products) contained in industrial effluents, treated effluents, environmental samples, etc., and take account of any additive and synergistic effects [37]. Furthermore, the use of bioassays involving organisms from different trophic levels is an efficient and essential tool for predicting environmental hazards to ecosystems [36,37].…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

et al. 2016

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This study investigated the aerobic biodegradation of methyl tertiary-butyl ether (MTBE) by a microbial consortium in a continuous up-flow packed-bed biofilm reactor using tezontle stone particles as a supporting material for the biofilm. Although MTBE is toxic for microbial communities, the microbial consortium used here was able to resist MTBE loading rates up to 128.3 mg L-1 h-1, with removal efficiencies of MTBE and chemical oxygen demand (COD) higher than 90%. A linear relationship was observed between the MTBE loading rate and the MTBE removal rate, as well as between the COD loading rate and the COD removal rate, within the interval of MTBE loading rates from 11.98 to 183.71 mg L-1 h-1. The metabolic intermediate tertiary butyl alcohol (TBA) was not detected in the effluent during all reactor runs, and the intermediate 2-hydroxy butyric acid (2-HIBA) was only detected at MTBE loading rates higher than 128.3 mg L-1 h-1. The results of toxicity bioassays with organisms from two different trophic levels revealed that the toxicity of the influent was significantly reduced after treatment in the packed-bed reactor. The packed-bed reactor system used in this study was highly effective for the continuous biodegradation of MTBE and is therefore a promising alternative for detoxifying MTBE-laden wastewater and groundwater.

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“…It is a fast, sensitive, and cost-effective method that can indicate the level of ecotoxicity of the tested substance. Although a complete ecotoxicology assessment requires biotests on organisms at each trophic level [1,11], Microtox is often chosen as the first biotest in a biotest battery based on speed and cost consideration [12].…”

Section: Introductionmentioning

confidence: 99%

Ecotoxicity assessment of camphor oxime using Microtox assay – preliminary research

et al. 2018

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The ecotoxicity assessment of new substances is crucial for their considered launch on the market. Due to various properties of chemical compounds, however, the application of biotests can be challenging. In the case of insoluble compounds, a proper, non-toxic solvent must be applied. The presented preliminary results demonstrate the extremely high ecotoxicity of camphor and camphor oxime, assessed by means of the Microtox system. A 81.9% Basic Test with 14 dilutions was applied. The ecotoxicity of a solvent (i.e. DMSO) was also assessed. The initial concentration of substances was 5%. EC50 values reached 0.00023% and 0.00007% for camphor and camphor oxime, respectively, after 15 min. of incubation of the samples with bioluminescent bacteria. The ecotoxicity of the solvent remained negligible. Due to the extremely high ecotoxicity values of the tested substances, another range of concentrations needs to be applied in future tests to confirm the results.

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Bioassays as one of the Green Chemistry tools for assessing environmental quality: A review

Cited by 84 publications

References 106 publications

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Development of the yeastSaccharomycescerevisiae as a biosensor for the toxicity detection of toxic substances

Biodegradation of Methyl Tertiary Butyl Ether (MTBE) by a Microbial Consortium in a Continuous Up-Flow Packed-Bed Biofilm Reactor: Kinetic Study, Metabolite Identification and Toxicity Bioassays

Ecotoxicity assessment of camphor oxime using Microtox assay – preliminary research

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