Bioassays with Vibrio fischeri for the assessment of delayed toxicity

Froehner, K.; Backhaus, Thomas; Grimme, L. Horst

doi:10.1016/s0045-6535(99)00274-x

Cited by 105 publications

(41 citation statements)

References 8 publications

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“…Such variation of effects with time of exposure (viz., cell division rate) was also observed for Pb, U, and Zn. The use of long-term exposure to contaminants has been suggested as a way to better assess toxicity when using bacteria (Dillon, 1993;Thomulka et al, 1993;Newman and McCloskey, 1996;Thomulka et al, 1996;Backhaus et al, 1997;Froehner et al, 2000). Such a long-term exposure Microtox -based bioassay has been developed, the Microtox Chronic Toxicity test (Bulich and Bailey, 1995;Bulich and Huynh, 1995;Johnson, 1998; see also http://www.…”

Section: Discussionmentioning

confidence: 99%

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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ABSTRACT:Metal toxicity is a function of the biology of the target organism and the chemical speciation of the metal. The toxicity of 11 metals was assessed with three cell-based bioassays based on marine organisms: the bacterium Photobacterium phosphoreum of the Microtoxா bioassay, an environmental strain of P. phosphoreum, and photocytes isolated from the brittlestar Ophiopsila californica. Metal speciation was calculated for three commonly used media: NaCl-based Microtoxா bioassay medium, artificial seawater glycerol, and artificial seawater. Decreased bioluminescence was considered a proxy for cell toxicity. In all three assays the elements Cd and Hg exhibited similar speciation as well as similar toxicity profiles. The element Cu was toxic in all three assays despite different metal speciation for the P. phosphoreum bioassay. The element Ag was toxic to both bacterial strains but not to photocytes despite a similar chemical speciation for all three assays. In general, the Microtoxா bioassay was sensitive to all metals (except Pb), whereas the photocytes were the least sensitive to the metals. The heightened response of the Microtoxா bioassay probably resulted from a combination of the limited complexing power of the medium and the greater sensitivity of the bacterial strain.

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Section: Discussionmentioning

confidence: 99%

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Deheyn

Bencheikh-Latmani

Latz

2004

Environmental Toxicology

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“…All tests were run as duplicates. The test utilizes the inhibition of the bioluminescence of the marine photobacteria, V. fischeri, as an indication of acute toxicity (Froehner et al, 2000;Jennings et al, 2001). All tests were conducted according to the German standard method DIN 38412; L34; L341, Germany and ISO DIS 11348 on a Dr. LANGE LUMIStox 300 photometer (Dü sseldorf, Germany).…”

Section: Methodsmentioning

confidence: 99%

Ozonation of oxytetracycline and toxicological assessment of its oxidation by-products

et al. 2008

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Antibiotic formulation effluents are well known for their difficult elimination by traditional bio-treatment methods and their important contribution to environmental pollution due to its fluctuating and recalcitrant nature. In the present study the effect of ozonation on the degradation of oxytetracycline (OTC) aqueous solution (100 mg l À1 ) at different pH values (3, 7 and 11) was investigated. Ozone (11 mg l À1 corresponds the concentration of ozone in gas phase) was chosen considering its rapid reaction and decomposition rate. The concentration of oxytetracycline, chemical oxygen demand (COD), biochemical oxygen demand (BOD) and BOD 5 /COD ratio were the parameters to evaluate the efficiency of the ozonation process. In addition, the toxic potential of the OTC degradation was investigated by the bioluminescence test using the LUMIStox 300 instrument and results were expressed as the percentage inhibition of the luminescence of the marine bacteria Vibrio fischeri. The results demonstrate that ozonation as a partial step of a combined treatment concept is a potential technique for biodegradability enhancement of effluents from pharmaceutical industries containing high concentration of oxytetracycline provided that the appropriate ozonation period is selected. At pH 11 and after 60 min of ozonation of oxytetracycline aqueous solutions (100 and 200 mg l À1 ) the BOD 5 /COD ratios were 0.69 and 0.52, respectively. It was also shown that COD removal rates increase with increasing pH as a consequence of enhanced ozone decomposition rates at elevated pH values. The results of bioluminescence data indicate that first by-products after partial ozonation (5-30 min) of OTC were more toxic than the parent compound.

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“…The toxicity of substances that affect biosynthetic pathways supporting growth and reproduction can only be determined if the bioassay covers the adequate period of the cell cycle. [56] In this sense, Kummerer et al [55] observed that EC50 values for ofloxacin and tetracycline decrease to 1-10 mg/L whereas the toxicity of metronidazole and sulfamethoxazole did not change after a prolonged exposure of 13 20 h. This fact must be taken into account before establishing the concentration limit to be treated in order to preserve the performance of an activated sludge unit.…”

Section: 1toxicitymentioning

confidence: 99%

Assessment of toxicity and biodegradability on activated sludge of priority and emerging pollutants

Tobajas

Verdugo

Polo

et al. 2015

Environmental Technology

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Bioassays with Vibrio fischeri for the assessment of delayed toxicity

Cited by 105 publications

References 8 publications

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Chemical speciation and toxicity of metals assessed by three bioluminescence‐based assays using marine organisms

Ozonation of oxytetracycline and toxicological assessment of its oxidation by-products

Assessment of toxicity and biodegradability on activated sludge of priority and emerging pollutants

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