Bioautography Detection of Antimicrobial Compounds from the Essential Oil of Salvia Pachystachys

Shakeri, Abolfazl; Sharifi, Mohammad J.; Bazzaz, Bibi Sedigheh Fazly; Emami, Seyed Ahmad; Soheili, Vahid; Sahebkar, Amirhossein; Asili, Javad

doi:10.2174/1573407212666161014132503

Cited by 29 publications

(19 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, it is important to understand that the bioautographic technique is not a quantitative measure for determining antimicrobial activity, since an indication of the number of compounds that have been separated and capable of inhibiting microbial growth is observed (Shakeri et al, 2018). In addition to the need for further investigation into the complex antimicrobial effects that these compounds present.…”

Section: Resultsmentioning

confidence: 99%

Investigação da atividade antimicrobiana de extratos e frações de espécies de Piper usando bioautografia

Souto

Rocha

Nunes³

et al. 2021

RSD

View full text Add to dashboard Cite

A necessidade do desenvolvimento de novos fármacos utilizados no combate e/ou controle dos micro-organismos permanece significativa no contexto científico mundial, sendo os produtos naturais, dentre eles os produtos vegetais, potenciais fontes de novos agentes antibacterianos e antifúngicos. Desse modo, o objetivo deste trabalho foi determinar a atividade antimicrobiana de espécies de Piper por bioautografia. Atividade antimicrobiana foi realizada pelo método da bioautografia, onde foram preparadas, previamente, cromatogramas pela técnica de cromatografia em camada delgada (CCD) pela eluição do extrato etanólico bruto (EEB), fração hexânica (HEX) e/ou fração clorofórmica (CHCl3) de P. caldense, P. arboreum e P. mollicomum, a fim de separar os metabólitos. As placas de CCD foram testadas diante de quatro cepas padrão American Type Culture Collection: Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853 e Candida albicans ATCC 76645, sendo a atividade determinada pela formação de zonas de inibição em Agar. Foi possível verificar que tanto o EEB como a fração HEX de Piper caldense foram ativos frente à cepa de S. aureus, as zonas de inibição foram observadas na cromatoplaca. No entanto estes produtos não apresentaram atividade diante das demais cepas estudadas. Os extratos e frações de Piper arboreum e Piper mollicomum não apresentaram atividade frente as cepas testadas. A partir dos resultados é possível concluir que Piper caldense possui potencial atividade antibacteriana frente a S. aureus. Ressalta-se a importância para que estudos futuros sejam realizados para identificação das substâncias ativas bem como seu teor em cada parte da planta.

show abstract

Section: Resultsmentioning

confidence: 99%

Investigação da atividade antimicrobiana de extratos e frações de espécies de Piper usando bioautografia

Souto

Rocha

Nunes³

et al. 2021

RSD

View full text Add to dashboard Cite

show abstract

“…The revitalized interest in utilizing natural products or creating synthetic versions of natural products grew in response to antibiotic resistance [ 11 , 12 , 13 , 14 ]. In recent years, phytochemicals have demonstrated strong antimicrobial activities in conjunction with standard antibiotic regimens [ 11 , 15 , 16 ]. Currently, alkaloids, organosulfur compounds, and phenolic compounds extracted from plants have shown efficacy against microbes, particularly multidrug-resistant bacteria.…”

Section: Mechanisms Of Antibacterial Activity and Resistancementioning

confidence: 99%

An Assessment of the In Vitro Models and Clinical Trials Related to the Antimicrobial Activities of Phytochemicals

2022

View full text Add to dashboard Cite

An increased number antibiotic-resistant bacteria have emerged with the rise in antibiotic use worldwide. As such, there has been a growing interest in investigating novel antibiotics against antibiotic-resistant bacteria. Due to the extensive history of using plants for medicinal purposes, scientists and medical professionals have turned to plants as potential alternatives to common antibiotic treatments. Unlike other antibiotics in use, plant-based antibiotics have the innate ability to eliminate a broad spectrum of microorganisms through phytochemical defenses, including compounds such as alkaloids, organosulfur compounds, phenols, coumarins, and terpenes. In recent years, these antimicrobial compounds have been refined through extraction methods and tested against antibiotic-resistant strains of Gram-negative and Gram-positive bacteria. The results of the experiments demonstrated that plant extracts successfully inhibited bacteria independently or in combination with other antimicrobial products. In this review, we examine the use of plant-based antibiotics for their utilization against antibiotic-resistant bacterial infections. In addition, we examine recent clinical trials utilizing phytochemicals for the treatment of several microbial infections.

show abstract

“…Hence, it is needed to develop a novel alternative or complementary antimicrobial drug which is safer and non-toxic to health ( Chitsazian-Yazdi et al., 2015 ). Herbal medicinal plants are a rich source of bioactive phytocompounds, which have potential against various diseases ( Shakeri et al., 2018 ). Many of these plants or phytochemical compounds are proven to be applied in therapeutics.…”

Section: Introductionmentioning

confidence: 99%

Tackling Multiple-Drug-Resistant Bacteria With Conventional and Complex Phytochemicals

Suganya

Packiavathy

Aseervatham³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Emerging antibiotic resistance in bacteria endorses the failure of existing drugs with chronic illness, complicated treatment, and ever-increasing expenditures. Bacteria acquire the nature to adapt to starving conditions, abiotic stress, antibiotics, and our immune defense mechanism due to its swift evolution. The intense and inappropriate use of antibiotics has led to the development of multidrug-resistant (MDR) strains of bacteria. Phytochemicals can be used as an alternative for complementing antibiotics due to their variation in metabolic, genetic, and physiological fronts as well as the rapid evolution of resistant microbes and lack of tactile management. Several phytochemicals from diverse groups, including alkaloids, phenols, coumarins, and terpenes, have effectively proved their inhibitory potential against MDR pathogens through their counter-action towards bacterial membrane proteins, efflux pumps, biofilms, and bacterial cell-to-cell communications, which are important factors in promoting the emergence of drug resistance. Plant extracts consist of a complex assortment of phytochemical elements, against which the development of bacterial resistance is quite deliberate. This review emphasizes the antibiotic resistance mechanisms of bacteria, the reversal mechanism of antibiotic resistance by phytochemicals, the bioactive potential of phytochemicals against MDR, and the scientific evidence on molecular, biochemical, and clinical aspects to treat bacterial pathogenesis in humans. Moreover, clinical efficacy, trial, safety, toxicity, and affordability investigations, current status and developments, related demands, and future prospects are also highlighted.

show abstract

Bioautography Detection of Antimicrobial Compounds from the Essential Oil of Salvia Pachystachys

Cited by 29 publications

References 0 publications

Investigação da atividade antimicrobiana de extratos e frações de espécies de Piper usando bioautografia

Investigação da atividade antimicrobiana de extratos e frações de espécies de Piper usando bioautografia

An Assessment of the In Vitro Models and Clinical Trials Related to the Antimicrobial Activities of Phytochemicals

Tackling Multiple-Drug-Resistant Bacteria With Conventional and Complex Phytochemicals

Contact Info

Product

Resources

About