Bioavailability and antioxidant capacity of plant extracts rich in polyphenols, given as a single acute dose, in sheep made highly susceptible to lipoperoxidation

Twenty Assaf lambs fed barley straw plus a concentrate alone (CONTROL group) or enriched with naringin (1.5 g/kg DM, NARINGIN group) were used to assess the effect of this polyphenolic compound on meat quality attributes. Serum samples were collected for 7 weeks, then the animals were slaughtered and the livers and longissimus thoracis et lumborum muscles extracted for analysis. Triacylglycerol levels in the serum samples tended to show (P 5 0.087) lower average values for the NARINGIN group when compared with the CONTROL, but no differences were observed when the meat was analysed for the intramuscular fat content. Lower thiobarbituric acid-reactive substances procedure (TBARS) values (P , 0.001) in the liver of the NARINGIN group were detected, probably as a consequence of naringenin accumulation in this organ. No significant differences were observed in the meat samples concerning TBARS or colour evolution during refrigerated storage, as not enough naringenin would have reached the muscle. Independent of naringin administration, the low levels of the most atherogenic oxysterols must be highlighted as the most important quality score in the lamb meat samples studied.

Section: Discussionmentioning

confidence: 99%

“…However, it has been suggested that different antioxidants given alone or combined with vitamin E could be more efficient at enhancing the antioxidant status of animals than the use of large doses of vitamin E (Gladine et al, 2007;Gobert et al, 2009 and.…”

Section: Introductionmentioning

confidence: 99%

The liver antioxidant status of fattening lambs is improved by naringin dietary supplementation at 0.15% rates but not meat quality

Bodas

Prieto

Jordán

et al. 2012

“…In this study, the lack of statistical difference between treatments could be due, first, to the large individual variability in cortisol concentration between animals, and second, to the low rates of naringin used in this experiment. In fact, Gladine et al (2007) observed an increase in plasma antioxidant status when sheep were supplemented with supranutritional doses (10%) of naringin. The stress experienced by the animal when transported to the slaughterhouse exacerbates the lipoperoxidation of polyunsaturated fatty acids (Kannan et al, 2000).…”

Section: Antioxidant Parametersmentioning

confidence: 96%

Naringin dietary supplementation at 0.15% rates does not provide protection against sub-clinical acidosis and does not affect the responses of fattening lambs to road transportation

López-Campos

Bodas

Prieto

et al. 2010

Forty Assaf fattening lambs (initial age 13 to 15 weeks) offered a diet of barley straw and a commercial concentrate were used to assess the effect of naringin (a type of citrus flavonoid with proven antioxidant, antimicrobial and anti-inflammatory properties in monogastric animals) at a dose of 1.5 g/kg per dry matteron plasma lipid peroxidation thiobarbituric acid reactive substances (TBARS), immune response, ruminal bacterial community and protection provided by the ruminal wall against subclinical acidosis. After 49 days of the experimental diets, lambs were subjected to a 4-h transportation stress period. As expected, TBARS values were significantly increased in all the lambs just after the transportation period, but no effect of naringin was observed. Although naringin lowered red blood cell count, neither the total white blood cells counts nor the production of IFN-g were affected by naringin. No anti-inflammation activity preventing rumenitis was detected, but a clear effect on ruminal bacterial community was observed in lambs consuming naringin. Further experiments, using different doses of naringin might show health benefits of naringin supplementation in lambs, but a clear beneficial effect on health was not readily apparent in this study.

“…For example, Gladine et al (2007), administered a single acute dose of plant extracts (rosemary, grape, citrus or marigold) directly into the rumen of sheep. The authors found that, after administration, epicatechin was detected in the plasma of animals dosed with grape extract, whereas naringenin was detected in the plasma of lambs dosed with citrus extract.…”

Section: Discussionmentioning

confidence: 99%

“…To date, only a few studies, in which animals were fed with high doses of phenolic compounds, showed that simple phenolics are partially bioavailable in ruminants. Gladine et al (2007) detected monomeric phenolic compounds in the plasma of sheep receiving a ruminal infusion of galloylated condensed tannins or simple phenolics from grape or rosemary extracts. Moñino et al (2008) reported that the muscle from lambs raised on milk from ewes fed with a rosemary-enriched concentrate contained several of the phenols from rosemary (i.e.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant effects of ryegrass phenolics in lamb liver and plasma

Luciano

Vasta

Gibson

et al. 2014

Sixteen lambs were divided into two groups and fed two different diets. Eight lambs were stall-fed with a concentrate-based diet (C), and the remaining eight lambs were allowed to graze on Lolium perenne (G). The antioxidant status was measured in the liver and plasma samples before and after solid-phase extraction (SPE) to probe the antioxidant effects that grass phenolic compounds may have conferred onto the animal tissues. The liver and plasma samples from grass-fed lambs displayed a greater antioxidant capacity than the tissues from C lamb group, but only if samples had not been passed through SPE cartridges. Finally, the feed and animal tissues, which had been purified by SPE, were analysed by liquid chromatography combined with mass spectrometry (LC-MS) to identify phenolic compounds present in L. perenne and to evaluate the results from the antioxidant assays. It would appear that the improvement of the antioxidant capacity of lamb liver and plasma from lambs fed ryegrass was not related to the direct transfer of phenolic compounds from grass to the animal tissues.