Biocalorimetry- supported analysis of fermentation processes

Meier-Schneiders, M.; Grosshans, U.; Busch, C.; Eigenberger, Gerhart

doi:10.1007/s002530050430

Cited by 4 publications

(6 citation statements)

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“…No further signal ®ltering or smoothing was required. The overall noise of q p (15 W, 75 mW/l) is not signi®cantly above the noise level published for bench-scale calorimeters, where values of 50 mW/l (van Klee et al, 1993), 20 mW/l (Meier-Schneiders et al, 1995a;Meier-Schneiders and Schafer, 1996) or 5 mW/l were achieved. This noise could be improved by adjusting the temperature controller, or reduced to 8 W (40 mW/l) simply by averaging data over 10 min.…”

Section: Batch Culture On Complex Mediummentioning

confidence: 61%

“…Developed ®rst for chemical processes (Karlsen and Villadsen, 1987;Landau, 1996;Regenass, 1985;Stockton et al, 1986), several researchers have adapted this technique to the study of living organisms (Cooney et al, 1969;Marison et al, 1998;Marison and von Stockar, 1985;Meier-Schneiders et al, 1992;van Klee et al, 1993). As a result they have become very powerful tools for quantitative thermodynamic studies (Duboc and von Stockar, 1995;Schill and von Stockar, 1995) and for the reliable monitoring and control of many bioprocesses (Birou et al, 1987;Duboc et al, 1999;Liu et al, 1999;Marison and von Stockar, 1986;Meier-Schneiders et al, 1995a;Randolph et al, 1989;van Klee et al, 1996;Voisard et al, 1998;von Stockar et al, 1997von Stockar et al, , 1995.…”

Section: Introductionmentioning

confidence: 99%

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Development of a large‐scale biocalorimeter to monitor and control bioprocesses

Voisard

Pugeaud

Kumar

et al. 2002

Biotech & Bioengineering

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Calorimetry has shown real potential at bench-scale for chemical and biochemical processes. The aim of this work was therefore to scale-up the system by adaptation of a standard commercially available 300-L pilot-scale bioreactor. To achieve this, all heat flows entering or leaving the bioreactor were identified and the necessary instrumentation implemented to enable on-line monitoring and dynamic heat balance estimation. Providing that the signals are sufficiently precise, such a heat balance would enable calculation of the heat released or taken up during an operational (bio)process. Two electrical Wattmeters were developed, the first for determination of the power consumption by the stirrer motor and the second for determination of the power released by an internal calibration heater. Experiments were designed to optimize the temperature controller of the bioreactor such that it was sufficiently rapid so as to enable the heat accumulation terms to be neglected. Further calibration experiments were designed to correlate the measured stirring power to frictional heat losses of the stirrer into the reaction mass. This allows the quantitative measurement of all background heat flows and the on-line quantitative calculation of the (bio)process power. Three test fermentations were then performed with B. sphaericus 1593M, a spore-forming bacterium pathogenic to mosquitoes. A first batch culture was performed on a complex medium, to enable optimization of the calorimeter system. A second batch culture, on defined medium containing three carbon sources, was used to show the fast, accurate response of the heat signal and the ability to perfectly monitor the different growth phases associated with growth on mixed substrates, in particular when carbon sources became depleted. A maximum heat output of 1100 W was measured at the end of the log-phase. A fed-batch culture on the same defined medium was then carried out with the feed rate controlled as a function of the calorimeter signal. A maximum heat output of 2250 W was measured at the end of the first log-phase. This work demonstrates that real-time quantitative calorimetry is not only possible at pilot-scale, but could be readily applied at even larger scales. The technique requires simple, readily available devices for determination of the few necessary heat flows, making it a robust, cost-effective technique for process development and routine monitoring and control of production processes.

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Section: Batch Culture On Complex Mediummentioning

confidence: 61%

Section: Introductionmentioning

confidence: 99%

Development of a large‐scale biocalorimeter to monitor and control bioprocesses

Voisard

Pugeaud

Kumar

et al. 2002

Biotech & Bioengineering

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“…Such a solution is much cheaper and more flexible than conventional reaction calorimeters or the tailor‐made fermentation calorimeters described by Meier‐Schneiders et al. (Meier‐Schneiders et al. , 1995a; Meier‐Schneiders and Schäfer, 1996).…”

Section: Information Content Of Calorimetric Signalsmentioning

confidence: 99%

What heat is telling us about microbial conversions in nature and technology: from chip‐ to megacalorimetry

Maskow

Кемп

Buchholz

et al. 2010

Microbial Biotechnology

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SummaryThe exploitation of microorganisms in natural or technological systems calls for monitoring tools that reflect their metabolic activity in real time and, if necessary, are flexible enough for field application. The Gibbs energy dissipation of assimilated substrates or photons often in the form of heat is a general feature of life processes and thus, in principle, available to monitor and control microbial dynamics. Furthermore, the combination of measured heat fluxes with material fluxes allows the application of Hess' law to either prove expected growth stoichiometries and kinetics or identify and estimate unexpected side reactions. The combination of calorimetry with respirometry is theoretically suited for the quantification of the degree of coupling between catabolic and anabolic reactions. New calorimeter developments overcome the weaknesses of conventional devices, which hitherto limited the full exploitation of this powerful analytical tool. Calorimetric systems can be integrated easily into natural and technological systems of interest. They are potentially suited for high‐throughput measurements and are robust enough for field deployment. This review explains what information calorimetric analyses provide; it introduces newly emerging calorimetric techniques and it exemplifies the application of calorimetry in different fields of microbial research.

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“…A highlight of Gerhart's experimental work is the development of a fermentation process calorimeter with a resolution of 20 mW/L that has been commercialized by industrial partners. 79 The fact that the apparatus design, the sensors, and the control have been developed in-house is indicative of the expertise available in his laboratory.…”

Section: In Honor Of Gerhart Eigenbergermentioning

confidence: 99%