Biocatalysis Based on Heme Peroxidases

Torres, Eduardo; Ayala, Marcela

doi:10.1007/978-3-642-12627-7

Cited by 48 publications

(1 citation statement)

References 201 publications

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“…Ascorbate peroxidase controls H 2 O 2 levels by oxidizing ascorbate in the first reaction of the ascorbate (ASC)-glutathione (GSH) cycle, a system that keeps H 2 O 2 levels under control (De Foyer and Noctor, 2011). Prxs are apoplastic and vacuolar plant peroxidases presenting a double catalytic-cycle, which allow them to both consume and produce reactive oxygen species (ROS; Chen and Schopfer, 1999;Zámocky and Obinger, 2010). Several reverse genetic studies, focused on the catalytic properties of these enzymes as well as on their specialized function, demonstrate that Prxs can be involved in a diverse range of functions (reviewed in Shigeto and Tsutstumi, 2016).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

et al. 2020

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Section: Introductionmentioning

confidence: 99%

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

et al. 2020

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Enhanced Functionality of Peroxidases by Its Immobilization at the Solid–Liquid Interface of Mesoporous Materials and Nanoparticles

Campos‐Terán

Iñarritu

Aburto

et al. 2013

Proteins in Solution and at Interfaces

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Synthesis and catalytic evaluation of Fe₃O₄/MWCNTs nanozyme as recyclable peroxidase mimetics: Biochemical and physicochemical characterization

Salarizadeh

Sadri

Sajedi

2017

Applied Organom Chemis

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In this research, the nanocomposite of multiwalled carbon nanotubes and magnetic metal oxide nanoparticles (Fe3O4/MWCNTs), as enzyme mimetic, was synthesized using an in situ chemical reduction method. The structure, composition and morphology of the prepared Fe3O4/MWCNT nanocomposite materials were characterized using X‐ray diffraction, FT‐IR and scanning electron microscopy with energy dispersive X‐ray spectroscopy, respectively. The magnetic properties of the nanocomposite were investigated by the vibrating sample magnetometer. A colorimetric system involving nanozyme, phenol/4‐aminoantipyrine and H2O2 was utilized for the determination of peroxidase mimetic catalytic assay. The obtained results confirmed that the synthesis of Fe3O4/MWCNTs nanostructures was successful. It was found that Fe3O4/MWCNTs nanohybrid exhibited peroxidase‐like activity without any pH limitation. Colorimetric data demonstrated that the prepared nanocatalyst had higher catalytic activity toward H2O2 than MWCNTs. The kinetic parameters of the nanozyme, Km and Vmax, were estimated to be 8.3 mm and 1.4 mm min−1, respectively. The Fe3O4/MWCNTs nanostructures were also successfully applied for glucose detection. In addition, peroxidase‐like activity of the nanozyme increased in the presence of butyl‐imidazolium bromide ionic liquid. These biomimetic catalysts have some advantages, such as simplicity, stability, reusability and cost effectiveness, which makes them great candidates to be used in various fields of biotechnology applications.

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Biocatalysis Based on Heme Peroxidases

Cited by 48 publications

References 201 publications

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

Enhanced Functionality of Peroxidases by Its Immobilization at the Solid–Liquid Interface of Mesoporous Materials and Nanoparticles

Synthesis and catalytic evaluation of Fe₃O₄/MWCNTs nanozyme as recyclable peroxidase mimetics: Biochemical and physicochemical characterization

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Biocatalysis Based on Heme Peroxidases

Cited by 48 publications

References 201 publications

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

Overexpression of ZePrx in Nicotiana tabacum Affects Lignin Biosynthesis Without Altering Redox Homeostasis

Enhanced Functionality of Peroxidases by Its Immobilization at the Solid–Liquid Interface of Mesoporous Materials and Nanoparticles

Synthesis and catalytic evaluation of Fe3O4/MWCNTs nanozyme as recyclable peroxidase mimetics: Biochemical and physicochemical characterization

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Synthesis and catalytic evaluation of Fe₃O₄/MWCNTs nanozyme as recyclable peroxidase mimetics: Biochemical and physicochemical characterization