Biocatalysis of agro-processing waste by marine Streptomyces fungicidicus strain RPBS-A4 for cellulase production

Abstract

The fundamental reason for this investigation is to lessen the production cost of cellulase by advancing the generation medium and utilizing an option carbon source such as agro-processing waste deposit. Streptomyces fungicidicus strain RPBS-A4 was subjected to fermentation medium with supplementation of agro-processing waste powder as a solid substrate for cellulase enzyme production. The impact of various process parameters was examined for increasing cellulase production. Cellulase was observed to be produced by S. fungicidicus strain RPBS-A4 using rice bran (5-6%), as best substrate among the tested. Solid state bioprocessing increased the production of enzyme activity at temperature -40°C, pH -9.0, inoculum size -0.6/100 ml, incubation period -96 h, substrate concentration -0.6 g/100 ml, carbon source -maltose (1%), and nitrogen source -yeast extract (1%). Under these most favorable conditions, the highest activity 13.18 U/ml of cellulase was observed. The S. fungicidicus RPBS-A4 has been considered as the best cellulase producer in biocatalysis of agro-processing waste deposits.