2012
DOI: 10.1016/j.biortech.2012.01.054
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Biocatalyst development by directed evolution

Abstract: Biocatalysis has emerged as a great addition to traditional chemical processes for production of bulk chemicals and pharmaceuticals. To overcome the limitations of naturally occurring enzymes, directed evolution has become the most important tool for improving critical traits of biocatalysts such as thermostability, activity, selectivity, and tolerance towards organic solvents for industrial applications. Recent advances in mutant library creation and high-throughput screening have greatly facilitated the engi… Show more

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Cited by 121 publications
(71 citation statements)
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“…Directed evolution is a powerful tool of protein engineering to design and modify the properties of enzymes [171]. This technology can be employed for a wide range of proteins, most of which are of interest for biocatalytic processes.…”
Section: Food Industry 306mentioning
confidence: 99%
“…Directed evolution is a powerful tool of protein engineering to design and modify the properties of enzymes [171]. This technology can be employed for a wide range of proteins, most of which are of interest for biocatalytic processes.…”
Section: Food Industry 306mentioning
confidence: 99%
“…In addition, Est-gela hydrolyzes (R,S)-ketoprofen ethyl ester preferably to (S)-ketoprofen than to (R)-ketoprofen. An ideal esterase, however, can produce only (S)-ketoprofen with high thermostability, and many studies on improvement of the thermostability and enantioselectivity of esterases have been conducted [58][59][60]. Accordingly, we are also trying to improve the thermostability and enantioselectivity of Est-gela using various mutagenesis methods (work in progress).…”
Section: Discussionmentioning
confidence: 96%
“…Many methods are available to create sequence diversity libraries, including chemical mutagenesis (34), error-prone PCR, saturation mutagenesis, and DNA shuffling (29,35,36,41). However, effective screening often remains as a bottleneck.…”
Section: Discussionmentioning
confidence: 99%