Biocatalytic Performance of Chloroperoxidase from Caldariomyces fumago Immobilized onto TiO2 Based Supports

Recent developments in the use of peroxygenases – Exploring their high potential in selective oxyfunctionalisations

“…The authors attributed this to a confinement effect that reduces enzyme unfolding. ( Muñoz-Guerrero et al, 2015 ) The molecular understanding of this effect needs to be improved. It has been shown that if an enzyme is deposited in a tight pore or shares a larger pore with many other enzymes, its folded structure I favoured over its unfolded form, which increases thermal stability and activity.…”

Magnetic separation and high reusability of chloroperoxidase entrapped in multi polysaccharide micro-supports

“…Different supports have been employed to improve CPO catalytic efficiency. Silica materials [23][24][25], iron oxide magnetic nanoparticles [26,27], Au@Fe 3 O 4 nanoparticles [28], nanostructured titanium oxide materials [29,30], ZnO nanowire/silica composite [31], alumina nanorods [32], an acrylic-based material (Eupergit C) [33] and graphene oxide nanosheets [34] are some of the most recently used carrier from the literature.…”

Catalytic Activity and Application of Immobilized Chloroperoxidase by Biometric Magnetic Nanoparticles

“…1.11.1.10), which is a versatile heme-containing enzyme, could catalyze sulfoxidation, epoxidation, dismutation, halogenation, and oxidation of numerous compounds with extensive high regioselectivity and enantioselectivity. , Such excellent catalytic properties enable CPO to have a great potential application in the synthesis of drugs and intermediates, but the lack of long-term operational stability has impeded its feasibility for practical industrial use. , Therefore, it is necessary to develop an effective strategy for improving the stability of CPO. , Immobilization is the most commonly used way to improve the stability of enzyme in industry. Various materials, such as mesoporous silica, − organic polymer matrices, , graphene oxide (GO) nanosheets, and nanotubes, , have been selected as supports to facilitate the stability and recycling of immobilized CPO. Although these methods could significantly enhance the stability of enzyme under harsh conditions, the efficient recovery and recycling of immobilized enzymes are still a short board for practical industrial application and must be improved.…”

“…3,4 Therefore, it is necessary to develop an effective strategy for improving the stability of CPO. 5,6 Immobilization is the most commonly used way to improve the stability of enzyme in industry. Various materials, such as mesoporous silica, 7−9 organic polymer matrices, 10,11 graphene oxide (GO) nanosheets, 12 and nanotubes, 5,13 have been selected as supports to facilitate the stability and recycling of immobilized CPO.…”