Biocatalytic reduction of prochiral aromatic ketones to optically pure alcohols by a coupled enzyme system for cofactor regeneration

Design and application of a novel ionic liquid with the property of strengthening coenzyme regeneration for whole-cell bioreduction in an ionic liquid-distilled water medium

“…It is adopted as a comparatively simple and accurate method to investigate the existence of NAD(P)H regeneration system by an enzyme activity assay, which was monitored by the detection of optical density variation at 340 nm in a reaction system containing cell-free extract, coenzyme and tested substrates (Yan et al, 2011). In our previous study, the carbonyl reductase derived from T. asperellum ZJPH0810 had been confirmed for exhibiting coenzyme specificity to NADH, and the NADH regeneration system was formed due to its asymmetric biocatalytic activity of BTAP to (R)-BTPE (Li et al, 2013).…”

Rhodotorula mucilaginosa as a new biocatalyst for asymmetric reduction of acetophenone

“…value stimulated by glucose have increased up to higher levels, 50.2 and 99.2% (Table 2). Yan et al (2011) thought that it is possible that multiple cofactor recycling systems run in parallel in cell by adding several hydrogen donors, and hence greatly enhance the cofactor regeneration. Thus, comparing with no co-substrate, conversion rate of acetophenone and e.e.…”