Biocementation for Sand Using an Eggshell as Calcium Source

Choi, Sun-Gyu; Wu, Shifan; Chu, Jian

doi:10.1061/(asce)gt.1943-5606.0001534

Cited by 111 publications

(51 citation statements)

References 13 publications

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“…The uniaxial compressive strengths ranged from 0.15 to 1.22 MPa (one sample at 3.6 MPa) and the CaCO3 contents from 0.6 to 7.5 wt%. In a comparable study using dissolved eggshells as a calcium source, Choi et al [24] reported compressive strengths in the range 0.3 to 0.5 MPa for CaCO3 contents between 5 and 8 %, which is consistent with our results.…”

Section: A Small-scale Mould Injection Strategy Yielded Solid Stablesupporting

confidence: 92%

Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Røyne

et al. 2019

Preprint

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The production of concrete for construction purposes is a major source of anthropogenic CO2 emissions. One promising avenue towards a more sustainable construction industry is to make use of naturally occurring mineral-microbe interactions, such as microbialinduced carbonate precipitation (MICP), to produce solid materials. In this paper, we present a new process where calcium carbonate in the form of powdered limestone is transformed to a binder material (termed BioZEment) through microbial dissolution and 2 recrystallization. For the dissolution step, a suitable bacterial strain, closely related to Bacillus pumilus, was isolated from soil near a limestone quarry. We show that this strain produces organic acids from glucose, inducing the dissolution of calcium carbonate in an aqueous slurry of powdered limestone. In the second step, the dissolved limestone solution is used as the calcium source for MICP in sand packed syringe moulds. The amounts of acid produced and calcium carbonate dissolved are shown to depend on the amount of available oxygen as well as the degree of mixing. Precipitation is induced through the pH increase caused by the hydrolysis of urea, mediated by the enzyme urease, which is produced in situ by the bacterium Sporosarcina pasteurii DSM33. The degree of successful consolidation of sand by BioZEment was found to depend on both the amount of urea and the amount of glucose available in the dissolution reaction.

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Section: A Small-scale Mould Injection Strategy Yielded Solid Stablesupporting

confidence: 92%

Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Røyne

et al. 2019

Preprint

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“…In order to achieve this aim and that of novel ground improvement technologies, increased collaboration between geotechnical engineers and geomicrobiologists will be required in order to explore more fully a wider range of microbial processes under both natural and engineered conditions. Cheng et al, , 2013Choi et al, 2016;Rowshanbakht et al, 2016;Terzis and Laloui, 2018;van Paassen et al, 2010) with comparable urease activity highlighted (Cheng et al, 2017). Figure 5.…”

Section: Discussionmentioning

confidence: 99%

“…Figure 5. Relationship between CaCO 3 content and unconfined compressive strength for studies in which saturation was either fully saturated or not recorded (grey circle outlines: (Al Cheng et al, 2017Choi et al, 2016;Rowshanbakht et al, 2016;Terzis and Laloui, 2018;van Paassen et al, 2010) with controlled saturation states highlighted (Cheng et al, 2013). Figure 6.…”

Section: Discussionmentioning

confidence: 99%

“…Whiffin 2004 Cheng et al, 2017Cheng et al, , 2013Choi et al, 2016;Rowshanbakht et al, 2016;Terzis and Laloui, 2018;van Paassen et al, 2010), data from these studies are also included in Figure 4 in order to understand the scale of variation. It should also be noted that differences in experimental procedure regarding carrying out UCS tests, can also lead to variability; some researchers use end caps to prepare perfectly flat ends, which can result in higher strengths being achieved than for specimens tested without the use of end caps.…”

Section: Reagentsmentioning

confidence: 99%

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Applications of Microbial Processes in Geotechnical Engineering

Mountassir

Minto

Paassen

et al. 2018

Advances in Applied Microbiology

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Over the last 10-15 years, a new field of "biogeotechnics" has emerged as geotechnical engineers seek to find ground improvement technologies which have the potential to be lower carbon, more ecologically friendly, and more cost-effective than existing practices. This review summarizes the developments which have occurred in this new field, outlining in particular the microbial processes which have been shown to be most promising for altering the hydraulic and mechanical responses of soils and rocks. Much of the research effort in this new field has been focused on microbially induced carbonate precipitation (MICP) via ureolysis, while a comprehensive review of MICP is presented here, the developments which have been made regarding other microbial processes, including MICP via denitrification and biogenic gas generation are also presented. Furthermore, this review outlines a new area of study: the potential deployment of fungi in geotechnical applications which has until now been unexplored.

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“…For example, several investigations [6][7][8] have confirmed that that the unconfined compressive strength (UCS) increases with the MICP-derived calcite content (CC). However, it has also been found that the hydraulic conductivity decreases as the CC increases [5,9,10]. Other researchers have evaluated biocementation using shear wave velocity [2,11,12], while yet another group of researchers have confirmed the levels of biocementation using triaxial [12,13] and dynamic testing [1,14].…”

Section: Introductionmentioning

confidence: 99%

Undrained Behavior of Microbially Induced Calcite Precipitated Sand with Polyvinyl Alcohol Fiber

2019

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Microbially induced calcite precipitation can cement sand and is an environment-friendly alternative to ordinary Portland cement. In this study, clean Ottawa sand was microbially treated to induce calcite contents (CCs) of 0%, 2%, and 4%. Polyvinyl alcohol fiber was also mixed with the sand at four different contents (0%, 0.2%, 0.4%, and 0.6%) with a constant CC of 4%. A series of undrained triaxial tests was conducted on the treated sands to evaluate the effects of the calcite treatment and fiber inclusion. Their hydraulic conductivity was also determined using a constant head test. As the CC increased from 0% to 4%, the friction angle and cohesion increased from 35.3° to 39.6° and from 0 to 93 kPa, respectively. For specimens with a CC of 4%, as the fiber content increased from 0% to 0.6%, the friction angle and cohesion increased from 39.6° to 42.8° and from 93 to 139 kPa, respectively. The hydraulic conductivity of clean Ottawa sand decreased by a factor of more than 100 as the CC increased from 0% to 4%. The fiber inclusion had less effect on the hydraulic conductivity of the specimen with 4% CC.

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Biocementation for Sand Using an Eggshell as Calcium Source

Cited by 111 publications

References 13 publications

Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Applications of Microbial Processes in Geotechnical Engineering

Undrained Behavior of Microbially Induced Calcite Precipitated Sand with Polyvinyl Alcohol Fiber

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Biocementation for Sand Using an Eggshell as Calcium Source

Cited by 111 publications

References 13 publications

Towards a low CO2 emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Towards a low CO2 emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Applications of Microbial Processes in Geotechnical Engineering

Undrained Behavior of Microbially Induced Calcite Precipitated Sand with Polyvinyl Alcohol Fiber

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Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production

Towards a low CO₂ emission building material employing bacterial metabolism in a two-step process of limestone dissolution and recrystallization: The bacterial system and prototype production