Biochemical analysis and photosynthetic activity of chloroplasts and Photosystem II particles from a barley mutant lacking chlorophyll b

Ouijja, Abderrahman; Farineau, Nicole; Cantrel, Catherine; Guillot‐Salomon, Thérèse

doi:10.1016/0005-2728(88)90143-0

Cited by 13 publications

(9 citation statements)

References 42 publications

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“…Leaf tips were often light green and sometimes with small brown spots indicating senescence. In addition, the tips were more susceptible to photoiohibition in both types of barley as indicated by PSII fluorescence (F^,/F^) in agreement with anatomical observations by Farineau et al (1988).…”

Section: Plant Materialssupporting

confidence: 87%

“…In contrast, it was hypothesized by Boardman and Highkin (1966) and Boardman and Thorne (1968) that the lower 0 found in their Chl fc-less barley was the result of a higher distribution of energy to PSI than to PSII. This occurs in spite of the apparently similar ratio of stacked to unstacked fhylakoids in Chl 6-iess and wild-type barley (Bassi et al 1985, Ouijja et al 1988); however, as indicated by these authors the Chl ft-less barley produces fewer, but larger grana than wild-type barley. The Chl 6-less mutant is reported to compensate for the unbalanced distribution of energy by increasing the ratio of the number of PSII to PSI reaction centers from 1.8 to 3.0 (Ghirardi et al 1986, Chow andAnderson 1991).…”

Section: Pigmentmentioning

confidence: 81%

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Photosynthesis and photoinhibition in leaves of chlorophyll b‐less barley in relation to absorbed light

Leverenz

Öquist

Wingsle

1992

Physiologia Plantarum

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The response of photosynthesis to absorbed light by intact leaves of wild‐type (Hordeum vulgare L. cv. Gunilla) and chlorophyll b‐less barley (H. vulgare L. cv. Dornaria, chlorina‐f22800) was measured in a light integrating sphere. Up to the section where the light response curve bends most sharply the responses of the b‐less and wild‐type barley were similar but not identical. Average quantum yield and convexity for the mutant light response curves were 0.89 and 0.90, respectively, times those of the wild‐type barley. The maximum quantum yield for PSII photochemistry was also 10% lower as indicated by fluorescence induction kinetics (Fv/Fm). Just above the region where the light curve bends most sharply, photosynthesis decreased with time in the mutant but not in the wild‐type barley. This decrease was associated with a decrease in Fv/Fm indicating photoinhibition of PSII. This photoinhibition occurred in the same region of the light response curve where zeaxanthin formation occurs. Zeaxanthin formation occurred in both the chlorophyll b‐less and wild‐type leaves. However, the epoxidation state was lower in the mutant than in the wild‐type barley. The results indicate that chlorophyll b‐less mutants will have reduced photosynthetic production as a result of an increased sensitivity to photoinhibition and possibly a lowered quantum yield and convexity in the absence of photoinhibition.

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Section: Plant Materialssupporting

confidence: 87%

Section: Pigmentmentioning

confidence: 81%

Photosynthesis and photoinhibition in leaves of chlorophyll b‐less barley in relation to absorbed light

Leverenz

Öquist

Wingsle

1992

Physiologia Plantarum

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“…However, the cases of three Chl-deficient mutants (sweetclover, barley, and rice) are inconsistent with the above pattern. These mutants are deficient or lacking in LHC2 complex, but still have normal stacking grana (Nakatani and Baliga 1985, Quijja et al 1988, Yang and Chen 1996. Our previous study of a red oval-pointed cecidomyiid gall derived from M. thunbergii leaf was a fourth exception (Yang et al 2003).…”

Section: Lhc2 Apoproteinsmentioning

confidence: 98%

Life time deficiency of photosynthetic pigment-protein complexes CP1, A1, AB1, and AB2 in two cecidomyiid galls derived from Machilus thunbergii leaves

Yang¹,

Yang²,

Huang³

et al. 2007

Photosynt.

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Two kinds of cecidomyiid galls induced by Daphnephila on Machilus thunbergii Sieb. & Zucc. leaves at various developmental stages, i.e., young, growing, and mature, were analyzed for their biochemical composition of photosynthetic pigment-protein complexes located in thylakoid membranes using the Thornber and MARS electrophoretic fractionation systems. Both kinds of galls were totally deficient in the pigment-protein complexes CP1, and A1, AB1, and AB2 through the whole period of gall formation. Immunoblotting of antibody against light-harvesting complex 2b (LHC2b) apoprotein confirmed this deficiency in gall's lifetime, which never recovered under any condition. Electron microscopy demonstrated that already at the early developmental stage the gall chloroplasts had thylakoid morphology like that in a normal leaf.

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“…Chl b, carotenoids), which facilitates absorption of visible wavelengths not absorbed by Chl a. Chlorophyll b is necessary for LHCII formation and for its stabilization. 34,42,43 An increase of LHCII in UV-B-irradiated plants might also provide enhanced photoprotection via higher concentrations of ~anthophylls.~~ Although phytochrome is usually thought to be redlfarred light regulated only, it has absorption peaks in the UV-B waveband and in the longwave UV-A (-0.380 km) region, in addition to the red Light of any wavelength absorbed by the chromophore (either Pr or Pfr) will convert it to the other form. Possibly more important to the physiological responses observed, the photochemical equilibrium of Pfr (mole fraction of total phytochrome), which describes the proportion of activated phytochrome is greater than 65% for the spectral region between 0.300 and 0.400 pm, and reaches -80% near 0.355 pm.47.48 Red, far-red, blue and physiologically active longwave UV-A radiation (0.350-0.400 pm, transmitted through glasshouse) were available to plants in all treatments from ambient sunlight, and the total available spectral radiation was the sum of artificial and natural sources.…”

Section: The Role Of Photoreceptorsmentioning

confidence: 99%

Understanding Photosynthesis, Pigment and Growth Responses Induced by Uv‐b and Uv‐a Irradiances

Middleton

Teramura

1994

Photochem & Photobiology

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Plant response to UV-B (0.290-0.320 pm) irradiation in controlled environments has been difficult to assess, possibly because plants also respond to UV-A (0.320-0.400 pm) and visible radiation. Photosynthetic dysfunction is often reported, but effects on photosynthetic pigments have been equivocal. Because UV-A/blue radiation is involved in pigment synthesis, the experimental UV-A irradiation was controlled and this study was conducted under high ambient photosynthetic photon flux (mid-day PPF 2 1400 pmol m--* s-l). Two biologically effective UV-B irradiances (10.7 and 14.1 kJ m-2 day-I) were utilized and the UV-A irradiances were matched in controls ( -5 and 9 kJ m-* day-'). Normal and two mutant pigment isolines (chlorophyll-deficient, flavonoid-deficient) of soybean cultivar Clark were utilized for comparisons. Many pigmedgrowth variables exhibited a statistical interaction between spectral quality and quantity. UV-A/blue photoregulation was demonstrated in the UV-A controls. The pigmentlgrowth pattern observed at the lower UV-B irradiance was interpreted as a photosystem I1 response similar to shade adaptation, suggesting phytochrome involvement in UV-B irradiation responses. On the other hand, two variables most commonly observed to manifest UV-B-induced effects-decreased photosynthesis and increased leaf flavonoid content-exhibited no interactions due to UV exposure or spectral quality. In general, the observed response patterns indicated either moderation of UV-B-induced responses by UV-A/blue radiation, or coaction between them, and provides an explanation for the common failure to demonstrate fluence-related responses in UV-B experiments.

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Biochemical analysis and photosynthetic activity of chloroplasts and Photosystem II particles from a barley mutant lacking chlorophyll b

Cited by 13 publications

References 42 publications

Photosynthesis and photoinhibition in leaves of chlorophyll b‐less barley in relation to absorbed light

Photosynthesis and photoinhibition in leaves of chlorophyll b‐less barley in relation to absorbed light

Life time deficiency of photosynthetic pigment-protein complexes CP1, A1, AB1, and AB2 in two cecidomyiid galls derived from Machilus thunbergii leaves

Understanding Photosynthesis, Pigment and Growth Responses Induced by Uv‐b and Uv‐a Irradiances

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