2005
DOI: 10.1074/jbc.m500264200
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Biochemical Analysis of the DNA Unwinding and Strand Annealing Activities Catalyzed by Human RECQ1*[boxs]

Abstract: RecQ helicases play an important role in preserving genomic integrity, and their cellular roles in DNA repair, recombination, and replication have been of considerable interest. Of the five human RecQ helicases identified, three are associated with genetic disorders characterized by an elevated incidence of cancer or premature aging: Werner syndrome, Bloom syndrome, and Rothmund-Thomson syndrome. Although the biochemical properties and protein interactions of the WRN and BLM helicases defective in Werner syndr… Show more

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Cited by 155 publications
(250 citation statements)
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“…We also tested whether RECQ1 (RECQL1), another member of the human RecQ family, has a stimulatory effect on RAD51. The biochemical properties of RECQ1 and BLM are similar; both proteins physically interact with RAD51 (41)(42)(43) and show branch migration activities (21,44). However, RECQ1 did not stimulate DNA strand exchange promoted by RAD51 under tested conditions; in contrast, at high concentrations RECQ1 inhibited this reaction (Fig.…”
Section: Blmmentioning
confidence: 86%
“…We also tested whether RECQ1 (RECQL1), another member of the human RecQ family, has a stimulatory effect on RAD51. The biochemical properties of RECQ1 and BLM are similar; both proteins physically interact with RAD51 (41)(42)(43) and show branch migration activities (21,44). However, RECQ1 did not stimulate DNA strand exchange promoted by RAD51 under tested conditions; in contrast, at high concentrations RECQ1 inhibited this reaction (Fig.…”
Section: Blmmentioning
confidence: 86%
“…The presence of a noncomplementary DNA double helix formed at the interface with the WH domain prompted us to ask whether this interaction could drive the annealing of cDNA strands in solution, possibly by forming a structural and electrostatic template for the formation of the initial, energetically unfavorable base pairs. DNA annealing activity has been observed with the full-length RECQ1 protein (23,24) and some other RecQ family members [BLM, RECQ5, and WRN (25-27)], as well as with proteins unrelated to RecQ (28). We decided to investigate the contribution of the WH domain to DNA annealing and unwinding by testing purified mutant proteins.…”
mentioning
confidence: 99%
“…Even when acting on the same substrates, they may do so by using different mechanisms (22). In addition to unwinding DNA, some RecQ helicases are also able to promote the annealing of complementary ssDNA molecules in an ATP-independent fashion (23)(24)(25)(26)(27)(28). Interestingly, a recent study indicated that different activities of RECQ1 are associated with distinct oligomeric forms: A form identified as monomers or dimers is responsible for DNA unwinding activity, whereas higher-order oligomers (hexamers or pentamers) possess strand-annealing activity (23).…”
mentioning
confidence: 99%