Biochemical and cytological analysis of five cultivars of Cicer (chickpea)

Abstract

During the present study, protein estimation and protein profiling of five cultivars of Cicer namely Dhawal, ujjwal, Shubhra, DCP-92-3 and IPC-04-20 have been carried out. The protein content on per gram fresh weight basis was found highest in the Dhawal measuring 35.2± 3.83 mg followed by DCP-92-3 and lowest was found in the IPC-04-20 cultivar. The protein profiling of seed proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a high polymorphism between the five cultivars. The total number of polypeptide bands recorded was 36, the 4 bands were monomorphic and the rest32 were polymorphic with an average polymorphism of 88.88%. The Jacards similarity ranged from 0.25000 to 0.56000. The similarity index calculated was highest between Ujjwal and IPC-04-20 (37.5%) where as lowest was calculated between Shubhra and IPC-04-20 (22.22%) variety. The unweighted pair group method with arithmetic mean (UPGMA) clustering method revealed two major clusters in the dendrogram that is, cluster 1 and 2, comprising two varieties each. The Shubhra occupies a distinct place as depicted in the dendrogram. Moreover, cytological studies of the five cultivars by calculating the mitotic index were carried out. The mitotic index varied between 4.82 to 10.83% and was found highest in DCP-92-3 while as minimum value was observed in Ujjwal.

Evaluation of Genetic Diversity within Some Cucurbitaceae Seeds from Nigeria using Electrophoresis

“…The banding patterns of the seven Cucurbitaceae species observed for Native-and SDS-PAGE data matrix were analysed for average polymorphism (Table 2) as proposed by Jahangir et al, (2014). In all the seven Cucurbitaceae species studied a total of 99 and 132 protein bands were detected respectively for water and buffer soluble proteins under Native-PAGE while a total of 218 and 123 protein bands were detected respectively under SDS-PAGE (Table 2).…”

“…The similarity index, based on the total seed protein patterns of melon species was calculated from the similarity values in the matrix (Silva and Russo, 2000) and using the Unweighted Pair-Group Method Analysis (UPGMA) algorithm proposed by Sokal and Michener (1958) cluster analyses were conducted and the resulting clusters were expressed as dendrograms. Average polymorphism was calculated as a ratio of total number of polymorphic bands (TNPB) to total number of bands (TNB) multiplied by 100 (TNPB/TNB x 100) while Jaccard's similarity index was calculated as ratio of similar bands to total bands between two species multiplied by 100 (Jahangir et al, 2014).…”