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Background: Assessment and molecular characterization of genetic diversity among the Dolichos lablab (L.) have huge implication in scheming strategies for breeding. In India, there is less comprehensive information on the potential genetic diversity of lablab bean and this is a major challenge for systematic use of lablab bean in genetic breeding programs. To exploit the available trait of interest, the genetic diversity of the locally available genotypes must be known. The PCR-based SSR approach requires just nanogram amounts of template DNA, subjected to quick detection and less influenced by environment. In this manner SSR have been utilizing broadly to study hereditary assorted qualities of yields.Methods: In this study, genetic diversity on ten Indian Dolichos lablab (L.) genotypes were surveyed utilizing SSR markers at Department of Botany, Bundelkhand University, Jhansi during 2019-20 got from Indian gene bank. For SSR analysis, 5 primers were used for checking the diversity in Dolichos lablab (L.) and the performance of markers were measured by using 4 parameters (1) PIC, (2) Mean resolving Power, (3) Resolving Power and (4) MI. Result: SSR (05) markers produced a total of 51 bands, out of which 45 bands (88.23%) were polymorphic which varies from 6 (Primer-111) to 15 (Primer-AGB-9) with mean of 10.5 bands per primer. The polymorphic information content value ranges from 0.265 to 0.488 with a mean value of 0.390. The value of Matrix index is 3.2. The value of resolving power ranges from 2.2 to 12.60 with a mean value of 7.40. In the UPGMA dendogram, the 10 genotypes were separated into two main clusters with all the primers used. Our present examination uncovered that genetic markers might be effectively used for deciding hereditary variety and connections in Dolichos lablab (L.) genotypes and could be utilized as a part of breeding programs.

During the present study, protein estimation and protein profiling of five cultivars of Cicer namely Dhawal, ujjwal, Shubhra, DCP-92-3 and IPC-04-20 have been carried out. The protein content on per gram fresh weight basis was found highest in the Dhawal measuring 35.2± 3.83 mg followed by DCP-92-3 and lowest was found in the IPC-04-20 cultivar. The protein profiling of seed proteins using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed a high polymorphism between the five cultivars. The total number of polypeptide bands recorded was 36, the 4 bands were monomorphic and the rest32 were polymorphic with an average polymorphism of 88.88%. The Jacards similarity ranged from 0.25000 to 0.56000. The similarity index calculated was highest between Ujjwal and IPC-04-20 (37.5%) where as lowest was calculated between Shubhra and IPC-04-20 (22.22%) variety. The unweighted pair group method with arithmetic mean (UPGMA) clustering method revealed two major clusters in the dendrogram that is, cluster 1 and 2, comprising two varieties each. The Shubhra occupies a distinct place as depicted in the dendrogram. Moreover, cytological studies of the five cultivars by calculating the mitotic index were carried out. The mitotic index varied between 4.82 to 10.83% and was found highest in DCP-92-3 while as minimum value was observed in Ujjwal.

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Dolichos lablab is a fancy plant in the U.S., an accepted food in Asia, Africa, and Central & S. America. Lablab seeds are no more than 1/2 inch in length at maximum & is coloured white to brown, red and black. It is a climbing plant with broad and large leaves. It condones the pH range of 5 to 7.5 and flourishes in a various type of soils like deep sands to heavy black clays. This study concentrates on the origin, chemical composition, distribution, pharmacology and taxonomic status of the aforementioned plant. The pharmacology study showed that D.