Simple Sequence Repeats Marker based Detection of Genetic Diversity of Indian Bean Dolichos lablab (L.) of Family Fabaceae

Abstract

Background: Assessment and molecular characterization of genetic diversity among the Dolichos lablab (L.) have huge implication in scheming strategies for breeding. In India, there is less comprehensive information on the potential genetic diversity of lablab bean and this is a major challenge for systematic use of lablab bean in genetic breeding programs. To exploit the available trait of interest, the genetic diversity of the locally available genotypes must be known. The PCR-based SSR approach requires just nanogram amounts of template DNA, subjected to quick detection and less influenced by environment. In this manner SSR have been utilizing broadly to study hereditary assorted qualities of yields.Methods: In this study, genetic diversity on ten Indian Dolichos lablab (L.) genotypes were surveyed utilizing SSR markers at Department of Botany, Bundelkhand University, Jhansi during 2019-20 got from Indian gene bank. For SSR analysis, 5 primers were used for checking the diversity in Dolichos lablab (L.) and the performance of markers were measured by using 4 parameters (1) PIC, (2) Mean resolving Power, (3) Resolving Power and (4) MI. Result: SSR (05) markers produced a total of 51 bands, out of which 45 bands (88.23%) were polymorphic which varies from 6 (Primer-111) to 15 (Primer-AGB-9) with mean of 10.5 bands per primer. The polymorphic information content value ranges from 0.265 to 0.488 with a mean value of 0.390. The value of Matrix index is 3.2. The value of resolving power ranges from 2.2 to 12.60 with a mean value of 7.40. In the UPGMA dendogram, the 10 genotypes were separated into two main clusters with all the primers used. Our present examination uncovered that genetic markers might be effectively used for deciding hereditary variety and connections in Dolichos lablab (L.) genotypes and could be utilized as a part of breeding programs.

SSR Marker-based Molecular Characterization of Lentil (Lens culinaris Medik.) Genotypes

“…Among different molecular markers, microsatellite markers have been employed in several studies to characterize genotypes in various crop species (Tiwari et al, 2019;Singh et al, 2022), however, their usage for genotypes of lentils has been limited due to their paucity (Wang et al, 2009;Weising et al, 2005). This method, which employs simple sequence repeat (SSR) markers in lentil genotypes, might be used as a quick and dynamic tool for finding polymorphism at the DNA level and analyzing genetic variation.…”

Evaluation of Proximate and Mineral Composition of Mutant Dolichos Lablab (Lablab purpureus L.) Accessions in Kenya

“…It is classified as an 'underutilized or orphaned crop' in Kenya which has contributed to limited research towards its improvement for nutritional and yield potential compared to main stream dry beans or common bean (Phaseolus vulgaris) and cowpea (Vigna unguiculata). Dolichos is more drought tolerant to common beans (Phaseolus vulgaris), soybean (Glycine max) [3,4] It also yields more grains compared to cowpea (Vigna unguiculata) [5].In Kenya dolichos lablab beans more market price from 2.5USD to 4USD per 2kg across markets compared to common which costs range from 0.8to 2.5USD. Therefore, Lablab purpureus can effectively substitute other legumes in Kenya [3] and [6].…”