1981
DOI: 10.1099/00221287-124-2-329
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Biochemical and Genetic Characterization of Streptomyces rimosus Mutants Impaired in Oxytetracycline Biosynthesis

Abstract: Antibiotic non-producing mutants were isolated from an oxytetracycline (OTC) producing strain of Streptomyces rimosus. Cosynthesis tests and feeding known intermediates of the OTC pathway allowed the classification of the mutants into several groups. The biosynthetic lesion was determined for several of the mutants. Some of the mutants deficient in the reduction of 5a,l la-dehydrooxytetracycline, the final step in the pathway, were unable to synthesize a cofactor (CSF 1) essential for this reaction. Mutations … Show more

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Cited by 40 publications
(55 citation statements)
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“…In the early 1970s, studies following three avenues of research were initiated. These were the biochemical characterization of non-OTC-producing mutants and mapping of the OTC genes (180,200), characterization and persistence of S. rimosus phages (104,107,193,225), and genetic and physical characterization of circular and linear S. rimosus plasmids (31,41,72,80,201,212).…”
Section: Introductionmentioning
confidence: 99%
“…In the early 1970s, studies following three avenues of research were initiated. These were the biochemical characterization of non-OTC-producing mutants and mapping of the OTC genes (180,200), characterization and persistence of S. rimosus phages (104,107,193,225), and genetic and physical characterization of circular and linear S. rimosus plasmids (31,41,72,80,201,212).…”
Section: Introductionmentioning
confidence: 99%
“…There are detailed studies on oxidation of C-6 [14,15], and chlorination at C-7 [16] in post-PKS tailoring steps of tetracycline biosynthesis. However, there is little information on the transamination reaction [17].…”
Section: Discussionmentioning
confidence: 99%
“…This OTC-blocked mutant was derived from S. rimosus M4018, which has been used for the commercial production of OTC [1]. The genetic lesion otcZ was first isolated by Rhodes et al [2] in autotrophic strains and then recombined into a prototrophs, which was used for this work. The strain was grown on trypticase soya broth (TSB) agar (30 g trypticase soya broth powder, 15 g agar, made up to 1 liter with distilled water) on Petri plates incubated at 30°C for 6ϳ10 days.…”
Section: Organism and Fermentationmentioning
confidence: 99%
“…defined gene group (otcZ) [2]. Subsequently, the otcZ151 mutant was shown to be complemented by a short segment of the otc cluster, when cloned in a low copy number vector [3].…”
mentioning
confidence: 99%