Biochemical and immunochemical characterization of the antigen–antibody reaction on a non-toxic biomimetic interface immobilized red blood cells of crucian carp and gold nanoparticles

“…At the same time, gold nanoparticles could be effectively immobilized onto the amino groups of the chitosan. In addition, the presence of nanogold provided a congenial microenvironment for the immobilized biomolecules and decreased the electron transfer impedance, leading to a direct electrochemical behavior of the immobilized HRP [20]. Thus, nanogold/Chi-MWNT substrate could exhibit more free range of motion to the immobilized proteins, increased the likelihood for interaction between the protein and the targeted antigens, and improved the sensitivity of the immunoassay system.…”

Section: Discussionmentioning

confidence: 99%

Conductometric immunosensors for the detection of staphylococcal enterotoxin B based bio-electrocalytic reaction on micro-comb electrodes

Chen

2007

Bioprocess Biosyst Eng

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Staphylococcal enterotoxin B (SEB) is one of many toxins produced by the Gram-positive bacterium Staphylococcal aureus. While SEB is known as the causative agent of certain food poisonings it is also considered abiological select agent. Thus, rapid and accurate identification of SEB during either surveillance or in response to a biothreat is critical to the mitigation of the suspect agent. This report presents a new conductometric immune-biosensor for the detection of SEB based on immobilization of horseradish peroxidase (HRP)-labeled SEB antibody (HRP-anti-SEB) onto nanogold/chitosan-multiwalled carbon nanotube (Au/CTS-MWNT)-functionalized biorecognition interface. The formation of the antibody-antigen complex by a simple one-step immunoreaction between the immobilized HRP-anti-SEB and SEB in sample solution introduced a barrier of electrical communication between the immobilized HRP and the base surface, thus local conductivity variations could be evaluated by the bio-electrocatalytic reaction of HRP in 0.02 M PBS (pH 6.8) containing 0.15 mM H(2)O(2), 0.06 M KI and 0.1 M NaCl. Under optimal conditions, the proposed immune-biosensor exhibited a good conductometric response relative to SEB concentration in a linear range from 0.5 to 83.5 ng/ml with a correlation coefficient of 0.998. The developed immune-biosensor showed an acceptable accuracy, reproducibility and stability. Milk samples spiked with various concentrations of SEB gave an average of 116% recovery of the toxin.

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“…Another competitive assay for CA 15-3 quantification in serum involves the use of red blood cells of crucian carp and AuNPs, which were deposited on a glassy carbon electrode surface, and bound to the antibodies, using HRP-CA 15-3 as tracer [87]. The red cells of crucian carp are not easy to break up in solution.…”

Section: Competitive Assaysmentioning

confidence: 99%