Biochemical and immunocytochemical identification of conglutin γ, a lupin seed lectin, in the roots of young germinating seedlings

Targeting carbohydrates: a novel paradigm for fungal control

“…The presence of large quantities of lectins in legume seeds that otherwise seem to fulfill a storage role remains a mystery. The proposition that they could function in the efficient packing of seed storage proteins inside the seed storage vacuoles was discarded due to the non-glycosylated nature of many legume globulins (Derbyshire et al 1976), and to the electrostatic-mediated, rather than lectin-mediated nature of the interaction between the glycosylated Lupinus seed storage proteins (α-, β-, and γ-conglutins; Ferreira et al 2003) and the lectin activity of some of these proteins: γ-conglutin (Duranti et al 1996) and blad, i.e. an intermediate stable breakdown product of β-conglutin catabolism (Ramos et al 1997).…”

Influence of temperature on secretion and functionality of lupin seed γ‐conglutin

“…γ-Conglutin is deposited in the protein bodies of mature seeds along with other storage proteins; however, it is not hydrolysed during seed germination. 5 Moreover, the molecular localisation of γ-conglutin in germinating seed is quite dynamic, thus, consideration of its role as a typical storage protein was excluded. 6 An intriguing phenomenon observed in the case of basic 7S globulins, a group of legume proteins to which γ-conglutin belongs, is secretion outside seeds in response to flooding and high temperature (50-65 °C).…”

“…γ ‐Conglutin is deposited in the protein bodies of mature seeds along with other storage proteins; however, it is not hydrolysed during seed germination 5 . Moreover, the molecular localisation of γ ‐conglutin in germinating seed is quite dynamic, thus, consideration of its role as a typical storage protein was excluded 6 .…”