Biochemical and molecular identification of a novel hepcidin type 2-like antimicrobial peptide in the skin mucus of the pufferfish Takifugu pardalis

Go, Hye‐Jin; Kim, Chan‐Hee; Park, Ji Been; Kim, Tae Young; Lee, Tae Kwan; Oh, Hye Young; Park, Nam Gyu

doi:10.1016/j.fsi.2019.08.017

Cited by 22 publications

(13 citation statements)

References 72 publications

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“…Skin mucus preparation was initiated following the procedures reported by Go et al [ 14 ]. C. argus was wounded by removing skin scales with sandpaper, and the mucus was collected nonlethally by anesthetizing the fish with 50 mg L −1 MS-222 (Sigma, St. Louis, MI, USA).…”

Section: Methodsmentioning

confidence: 99%

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Leng

Shi

et al. 2021

Foods

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Microbial contamination is one of the most common food safety issues that lead to food spoilage and foodborne illness, which readily affects the health of the masses as well as gives rise to huge economic losses. In this study, Channa argus was used as a source of antimicrobial agent that was then analyzed by untargeted metabolomics for its antibacterial mechanism against Staphylococcus aureus. The results indicated that the skin mucus extract of C. argus had great inhibitory action on the growth of S. aureus, and the morphology of S. aureus cells treated with the skin mucus extract exhibited severe morphological damage under scanning electron microscopy. In addition, metabolomics analysis revealed that skin mucus extract stress inhibited the primary metabolic pathways of S. aureus by inducing the tricarboxylic acid cycle and amino acid biosynthesis, which further affected the normal physiological functions of biofilms. In conclusion, the antimicrobial effect of the skin mucus extract is achieved by disrupting cell membrane functions to induce an intracellular metabolic imbalance. Hence, these results conduce to amass novel insights into the antimicrobial mechanism of the skin mucus extract of C. argus against S. aureus.

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Section: Methodsmentioning

confidence: 99%

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Leng

Shi

et al. 2021

Foods

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“…While the encoding peptide (PChepc) by the hampII of large yellow croaker could kill all tested gram-positive and gram-negative bacteria at very low concentrations (Table 1). It has been reported that most fish hampIIs have a potent broad-spectrum antibacterial activity (16,17,27,31,38). For example, the HampII peptides from Dicentrarchus labrax, could significantly inhibited the growth of Photobacterium damselae, Streptococcus parauberis and Lactococcus garviae at a low protein concentration of 1 mM (16).…”

Section: Biological Activity Detection Of Seahorse Hehampii Peptidesmentioning

confidence: 99%

“…Hepcidin is a small cysteine-rich antimicrobial peptide, including two types: hepcidin I and II. The hepcidin I (HampI) is a key regulator of iron metabolism for vertebrates, while hepcidin II (HampII) is considered to mainly function as immune defence in fish (16,17). HampII only present in teleost fishes with multiple copies, which is a result of genome duplications and tandem duplications (18,19).…”

Section: Introductionmentioning

confidence: 99%

Hepcidin Gene Co-Option Balancing Paternal Immune Protection and Male Pregnancy

et al. 2022

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Viviparity has originated independently more than 150 times in vertebrates, while the male pregnancy only emerged in Syngnathidae fishes, such as seahorses. The typical male pregnancy seahorses have closed sophisticated brood pouch that act as both uterus and placenta, representing an excellent model system for studying the evolutionary process of paternal immune protection. Phylogenetic analysis indicated that the hampII gene family has multiple tandem duplicated genes and shows independent lineage-specific expansion in seahorses, and they had the highest ratio of nonsynonymous substitutions to synonymous substitutions (dN/dS) in the seahorse phylogenetic branch. The expression levels of hampIIs in the brood pouch placenta were significantly higher during pregnancy than non-pregnancy. Both LPS stimulation test in vivo and cytotoxicity test in vitro proved the immunological protection function of hampIIs against pathogen infection in seahorse. Besides, seahorse hampII peptides exhibit weaker antibacterial function, but stronger agglutination and free endotoxin inhibition. We assumed that the modified immunological function seemed to be a trade-off between the resistance to microbial attack and offspring protection. In brief, this study suggests that the rapid co-option of hampIIs contributes to the evolutionary adaption to paternal immune care during male pregnancy.

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“…The alignment depicts a number of conserved motifs, particularly between the first pufferfish hepcidin sequence (various pufferfish species have at least two hepcidin-like genes [55]) and the coronavirus spike proteins. In a sense, the pufferfish sequence seems to act as a 'bridge' between the coronavirus motifs and those in the human hepcidin sequence.…”

Section: Search For Sequence Similaritymentioning

confidence: 99%

COVID-19 and iron dysregulation: distant sequence similarity between hepcidin and the novel coronavirus spike glycoprotein

Ehsani

2020

Biol Direct

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The spike glycoprotein of the SARS-CoV-2 virus, which causes COVID-19, has attracted attention for its vaccine potential and binding capacity to host cell surface receptors. Much of this research focus has centered on the ectodomain of the spike protein. The ectodomain is anchored to a transmembrane region, followed by a cytoplasmic tail. Here we report a distant sequence similarity between the cysteine-rich cytoplasmic tail of the coronavirus spike protein and the hepcidin protein that is found in humans and other vertebrates. Hepcidin is thought to be the key regulator of iron metabolism in humans through its inhibition of the iron-exporting protein ferroportin. An implication of this preliminary observation is to suggest a potential route of investigation in the coronavirus research field making use of an already-established literature on the interplay of local and systemic iron regulation, cytokine-mediated inflammatory processes, respiratory infections and the hepcidin protein. The question of possible homology and an evolutionary connection between the viral spike protein and hepcidin is not assessed in this report, but some scenarios for its study are discussed.

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Biochemical and molecular identification of a novel hepcidin type 2-like antimicrobial peptide in the skin mucus of the pufferfish Takifugu pardalis

Cited by 22 publications

References 72 publications

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Untargeted Metabolomics on Skin Mucus Extract of Channa argus against Staphylococcus aureus: Antimicrobial Activity and Mechanism

Hepcidin Gene Co-Option Balancing Paternal Immune Protection and Male Pregnancy

COVID-19 and iron dysregulation: distant sequence similarity between hepcidin and the novel coronavirus spike glycoprotein

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