Biochemical and Morphological Features of Rice Cell Death Induced by Pseudomonas avenae

Che, Fang‐Sik; Iwano, Megumi; Tanaka, Noriko; Takayama, Seiji; Minami, Eiich; Shibuya, Naoto; Kadota, Ikuo; Isogai, Akira

doi:10.1093/oxfordjournals.pcp.a029485

Cited by 45 publications

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“…Bacterial Strains and Cell Extracts-P. avenae strains H8301 (MAFF 301505) and K1 isolated from rice and strain N1141 (MAFF 301141) isolated from finger millet (22) were maintained on Pseudomonas F agar plates (Difco) at 30°C (23).…”

Section: Methodsmentioning

confidence: 99%

“…The accumulation of EL2 mRNA is detectable 3 h after inoculation with the incompatible strain, N1141, whereas in H8301-inoculated cultured cells it is not detected. From these results, we concluded that the N1141 strain causes a defense response in cultured rice cells but that the compatible strain H8301 does not (23). However, the perception mechanism of the compatible or the incompatible strain by rice and the induction mechanism of the defense response caused by the incompatible strain of P. avenae remain unsolved.…”

mentioning

confidence: 92%

“…The host range of P. avenae is wide among monocotyledonous plants including rice, oats, Italian millet, and maize; however, individual strains of the pathogen infect only one or a few host species (20 -22). For example, strains isolated from rice such as H8301 (MAFF 301505) or K1 can infect only rice plant, while the N1141 (MAFF 301141) strain isolated from finger millet cannot infect rice even after being inoculated into rice tissues (20,21,23). We recently reported that the riceincompatible strain, N1141, caused rapid cell death, while the rice-compatible strain, H8301, did not induce it (23).…”

mentioning

confidence: 99%

“…For example, strains isolated from rice such as H8301 (MAFF 301505) or K1 can infect only rice plant, while the N1141 (MAFF 301141) strain isolated from finger millet cannot infect rice even after being inoculated into rice tissues (20,21,23). We recently reported that the riceincompatible strain, N1141, caused rapid cell death, while the rice-compatible strain, H8301, did not induce it (23). Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) showed that DNA cleavage occurred during rapid cell death induced by N1141 strain.…”

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confidence: 99%

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Flagellin from an Incompatible Strain of Pseudomonas avenae Induces a Resistance Response in Cultured Rice Cells

Sik¹,

Nakajima²,

Tanaka³

et al. 2000

Journal of Biological Chemistry

116

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The host range of Pseudomonas avenae is wide among monocotyledonous plants, but individual strains can infect only one or a few host species. The resistance response of rice cells to pathogens has been previously shown to be induced by a rice-incompatible strain, N1141, but not by a rice-compatible strain, H8301. To clarify the molecular mechanism of the host specificity in P. avenae, a strain-specific antibody that was raised against N1141 cells and then absorbed with H8301 cells was prepared. When a cell extract of strain N1141 was separated by SDS-polyacrylamide gel electrophoresis and immunostained with the N1141 strain-specific antibody, only a flagellin protein was detected. Purified N1141 flagellin induced the hypersensitive cell death in cultured rice cells within 6 h of treatment, whereas the H8301 flagellin did not. The hypersensitive cell death could be blocked by pretreatment with anti-N1141 flagellin antibody. Furthermore, a flagellin-deficient N1141 strain lost not only the induction ability of hypersensitive cell death but also the expression ability of the EL2 gene, which is thought to be one of the defenserelated genes. These results demonstrated that the resistance response in cultured rice cells is induced by the flagellin existing in the incompatible strain of P. avenae but not in the flagellin of the compatible strain.

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Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 92%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Flagellin from an Incompatible Strain of Pseudomonas avenae Induces a Resistance Response in Cultured Rice Cells

Sik¹,

Nakajima²,

Tanaka³

et al. 2000

Journal of Biological Chemistry

116

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show abstract

“…24 However in plants, laddering was only reported in some cases 25,26 but not in others. 27,28 These discrepancies might be due to the timing of sampling or there might be some differences between different types of PCD.…”

Section: Elucidating the Cell Death Machinerymentioning

confidence: 99%

Lesion mimic mutants

Moeder

Yoshioka²

2008

Plant Signaling & Behavior

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Apoptotic Cell Death and Cellular Surface Negative Charge Increase in Maize Roots Exposed to Cytotoxic Stresses

Ning

Wang

et al. 2001

Annals of Botany

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Biochemical and Morphological Features of Rice Cell Death Induced by Pseudomonas avenae

Cited by 45 publications

References 0 publications

Flagellin from an Incompatible Strain of Pseudomonas avenae Induces a Resistance Response in Cultured Rice Cells

Flagellin from an Incompatible Strain of Pseudomonas avenae Induces a Resistance Response in Cultured Rice Cells

Lesion mimic mutants

Apoptotic Cell Death and Cellular Surface Negative Charge Increase in Maize Roots Exposed to Cytotoxic Stresses

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