Biochemical and pathophysiological properties of polyamines

Agostinelli, Enzo

doi:10.1007/s00726-020-02821-8

Cited by 21 publications

(10 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biologically, PAs and their analogues possess functional involvements in human health and diseases, such as gastroenterology [ 21 ], oncology [ 22 , 23 ], oxidative stress, cerebral strokes [ 24 ], parasitology [ 25 ], apoptosis [ 26 , 27 ], obesity [ 28 ], asthma [ 29 ], and other ailments [ 2 ]. SPE and SPD work as the substrates for different biological enzymes to form cytotoxic metabolites by the activity of spermine oxidase (SMO), monoamine oxidase (MAO), copper amine oxidase (CuAOs), and polyamine oxidases (PAO) [ 30 , 31 ]. Amine oxidases (ASOs) are the key molecules behind the regulation of Mono, di, N-acyl amines, and PAs.…”

Section: Introductionmentioning

confidence: 99%

“…H 2 O 2 and aldehyde(s) cross the inner mitochondrial membrane and react with endogenous structures and molecules in order to induce the death of tumor cells [ 32 ]. In vitro cytotoxicity can be induced in the presence of internal PAs or external SPE in various tumor cell lines of humans using CuAOs, i.e., bovine serum amine oxidase [ 31 , 32 ]. It can also be performed in vivo using CuAOs injection in the tumor [ 33 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Polyamines: Functions, Metabolism, and Role in Human Disease Management

et al. 2021

View full text Add to dashboard Cite

Putrescine, spermine, and spermidine are the important polyamines (PAs), found in all living organisms. PAs are formed by the decarboxylation of amino acids, and they facilitate cell growth and development via different cellular responses. PAs are the integrated part of the cellular and genetic metabolism and help in transcription, translation, signaling, and post-translational modifications. At the cellular level, PA concentration may influence the condition of various diseases in the body. For instance, a high PA level is detrimental to patients suffering from aging, cognitive impairment, and cancer. The levels of PAs decline with age in humans, which is associated with different health disorders. On the other hand, PAs reduce the risk of many cardiovascular diseases and increase longevity, when taken in an optimum quantity. Therefore, a controlled diet is an easy way to maintain the level of PAs in the body. Based on the nutritional intake of PAs, healthy cell functioning can be maintained. Moreover, several diseases can also be controlled to a higher extend via maintaining the metabolism of PAs. The present review discusses the types, important functions, and metabolism of PAs in humans. It also highlights the nutritional role of PAs in the prevention of various diseases.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Polyamines: Functions, Metabolism, and Role in Human Disease Management

et al. 2021

View full text Add to dashboard Cite

show abstract

“…A possible role in the regulation of sperm apoptosis may be attributed to these polycationic molecules (35,36). The antiapoptotic and cytoprotective effect of PA upon cells has been well documented (8,(37)(38)(39)(40)(41). The MW irradiation of specific frequencies may induce apoptosis, and this effect is studied as a possible therapeutic tool for the management of cancer (42).…”

Section: Spermatozoa Percentage % -mentioning

confidence: 99%

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Плосконос

Zulbalaeva²,

Kurbangalieva³

et al. 2022

Biomed Rep

View full text Add to dashboard Cite

The aim of the present study was to investigate the influence of millimeter-wave electromagnetic (MW) irradiation on normal and pathological human sperm in vitro, and to evaluate a possible role of polyamines (PA) in this process. The stability of sperm membranes, the number of apoptotic gametes, and the content of seminal plasma PA in the ejaculates of fertile and subfertile men were compared before and after short-term MW electromagnetic exposure in vitro. The ejaculate samples were collected from healthy donors [n=25, age 22-38 years old (y.o.), average age 30.6±1.1 y.o. (mean ± SEM)] and from subfertile men (n=78, age 25-48 y.o., average age 34.1±0.8 y.o.) and exposed to MW radiation. The electromagnetic field had a wavelength of 7.1 mm, a frequency of 42.194 GHz and an exposure time of 20 min. The fragility of sperm membranes was evaluated by their resistance to sodium chloride solution (Milovanov test) and to acetic acid (Joel test). Acrosin activity was assayed spectrophotometrically. Apoptosis was determined by the externalization of phosphatidylserine on the outer side of the sperm membrane and propidium iodide staining. The PA levels were determined by agar gel electrophoretic fractionation. An increase in the resistance of sperm membranes, a decrease in acrosin activity, a decrease in the number of apoptotic gametes and a decrease in the seminal plasma PA concentrations were found after exposure of the native human sperm to low-intensity MW irradiation. Two types of reactions were revealed for the subfertile samples. The results revealed positive bio-effects of specific microwaves on the human semen and the participation of PA in the realization of these effects.

show abstract

“…Angiogenesis provides the physical conduit for utero-placental blood flow to enhance mother to fetus exchanges of water, AAs, and other nutrients, as well as gases and wastes [ 11 ]. Arg is also known to be a precursor for syntheses of polyamines, ornithine, creatine, agmatine, and homoarginine, each of which has enormous physiological significance [ 18 – 21 ]. These biologically important substances are essential for conceptus growth and survival [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae

Herring

Bazer

Johnson

et al. 2022

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Most embryonic loss in pigs occurs before d 30 of gestation. Dietary supplementation with L-arginine (Arg) during early gestation can enhance the survival and development of conceptuses (embryo/fetus and its extra-embryonic membranes) in gilts. However, the underlying mechanisms remain largely unknown. Methods Between d 14 and 30 of gestation, each gilt was fed daily 2 kg of a corn- and soybean-meal based diet (12% crude protein) supplemented with either 0.4% Arg (as Arg-HCl) or an isonitrogenous amount of L-alanine (Control). There were 10 gilts per treatment group. On d 30 of gestation, gilts were fed either Arg-HCl or L-alanine 30 min before they were hysterectomized, followed by the collection of placentae, embryos, fetal membranes, and fetal fluids. Amniotic and allantoic fluids were analyzed for nitrite and nitrate [NOx; stable oxidation products of nitric oxide (NO)], polyamines, and amino acids. Placentae were analyzed for syntheses of NO and polyamines, water and amino acid transport, concentrations of amino acid-related metabolites, and the expression of angiogenic factors and aquaporins (AQPs). Results Compared to the control group, Arg supplementation increased (P < 0.05) the number of viable fetuses by 1.9 per litter, the number and diameter of placental blood vessels (+ 25.9% and + 17.0% respectively), embryonic survival (+ 18.5%), total placental weight (+ 36.5%), the total weight of viable fetuses (+ 33.5%), fetal crown-to-rump length (+ 4.7%), and total allantoic and amniotic fluid volumes (+ 44.6% and + 75.5% respectively). Compared to control gilts, Arg supplementation increased (P < 0.05) placental activities of GTP cyclohydrolase-1 (+ 33.1%) and ornithine decarboxylase (+ 29.3%); placental syntheses of NO (+ 26.2%) and polyamines (+ 28.9%); placental concentrations of NOx (+ 22.5%), tetrahydrobiopterin (+ 21.1%), polyamines (+ 20.4%), cAMP (+ 27.7%), and cGMP (+ 24.7%); total amounts of NOx (+ 61.7% to + 96.8%), polyamines (+ 60.7% to + 88.7%), amino acids (+ 39% to + 118%), glucose (+ 60.5% to + 62.6%), and fructose (+ 41.4% to + 57.0%) in fetal fluids; and the placental transport of water (+ 33.9%), Arg (+ 78.4%), glutamine (+ 89.9%), and glycine (+ 89.6%). Furthermore, Arg supplementation increased (P < 0.05) placental mRNA levels for angiogenic factors [VEGFA120 (+ 117%), VEGFR1 (+ 445%), VEGFR2 (+ 373%), PGF (+ 197%), and GCH1 (+ 126%)] and AQPs [AQP1 (+ 280%), AQP3 (+ 137%), AQP5 (+ 172%), AQP8 (+ 165%), and AQP9 (+ 127%)]. Conclusion Supplementing 0.4% Arg to a conventional diet for gilts between d 14 and d 30 of gestation enhanced placental NO and polyamine syntheses, angiogenesis, and water and amino acid transport to improve conceptus development and survival.

show abstract

Biochemical and pathophysiological properties of polyamines

Cited by 21 publications

References 75 publications

Polyamines: Functions, Metabolism, and Role in Human Disease Management

Polyamines: Functions, Metabolism, and Role in Human Disease Management

Assessing the biological effects of microwave irradiation on human semen in vitro and determining the role of seminal plasma polyamines in this process

Dietary supplementation with 0.4% L-arginine between days 14 and 30 of gestation enhances NO and polyamine syntheses and water transport in porcine placentae

Contact Info

Product

Resources

About