Biochemical Approaches to Monitoring Human Populations for Germinal Mutation rates: I. Electrophoresis

Neel, James V.; Mohrenweiser, Harvey W.; Hanash, Samir M.; Rosenblum, Barnett B.; Sternberg, S.; Wurzinger, K. H.; Rothman, Edward D.; Satoh, Chiyoko; Goriki, Kazuo; Krasteff, Todor; Long, Michael W.; Skolnick, M.; Krzesicki, Raymond F.

doi:10.1007/978-1-4613-3739-3_6

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…This convention carries with it the risk of missing a mutation whose phenotype mimics a common polymorphism. Elsewhere we have calculated that the underestimation of mutation rates which results from this omission should be about 10% (Neel et al, 1983). On the other hand, undertaking a family investigation to test whether a common polymorphism was the result of a mutational event in a parent would have increased the work load of family studies by an order of magnitude basd on the actual polymorphism frequencies in these data.…”

Section: Discussionmentioning

confidence: 98%

“…Blood samples were collected and processed as described by Neel et al (1979) and Fielek and Mohrenweiser (1979). An aliquot of cells was retained in a glycerol-sorbitol mixture for blood typing (Weiner and Lourie, 1981).…”

Section: Methodsmentioning

confidence: 99%

“…On the other hand, the data could be --and have been --pooled with other appropriate data to yield such an estimate . A major emphasis of the Ann Arbor effort was the development and/or adaptation of biochemical methods for detecting germinal mutation, with the satisfactory methodologies to be introduced subsequently into the laboratories of the Radiation Effects Research Foundation in Hiroshima and Nagasaki, as the basis for the much larger study of the offspring of atomic bomb survivors (Neel et al, 1979(Neel et al, , 1980. Finally, the data resulting from the study will eventually be incorporated into a treatment of the comparative genetics of a variety of ethnic groups.…”

Section: Source Of Samplesmentioning

confidence: 99%

“…It is worth emphasizing at the outset that these 'lessons' will in many respects also apply to any similar study of mutation utilizing such other approaches as the examination of proteins by two-dimensional polyacrylamide gel elec-trophoresis or of DNA by the various available methodologies. Preliminary references to this study have appeared in Neel et al (1979Neel et al ( , 1980 and Mohrenweiser (1981Mohrenweiser ( , 1983a.…”

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confidence: 99%

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A pilot study of the use of placental cord blood samples in monitoring for mutational events

Neel

Mohrenweiser

Gershowitz

1988

Mutation Research/Genetic Toxicology

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A pilot study exploring the examination of placental cord blood samples for mutant proteins with one-dimensional electrophoretic techniques is described. Although technical advances are such that the techniques employed in this study are now partially superceded, the practical problems encountered in this study would be typical of any monitoring program of this type. No mutations altering electrophoretic mobility among a battery of 51 different locus products were encountered in a total of 277,747 locus tests. When these data are combined with similar data from other studies, the mutation rate for electromorphs becomes 0.34 X 10(-5)/locus/generation.

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Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

Section: Source Of Samplesmentioning

confidence: 99%

mentioning

confidence: 99%

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A pilot study of the use of placental cord blood samples in monitoring for mutational events

Neel

Mohrenweiser

Gershowitz

1988

Mutation Research/Genetic Toxicology

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Detection of human somatic cell structural gene mutations by two‐dimensional electrophoresis

et al. 1987

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The feasibility of detecting human somatic structural gene mutations by two dimensional electrophoresis has been investigated. A lymphoblastoid cell line was grown as a mass culture in the presence of ethylnitrosourea, after which cells were regrown as single cell clones. A total of 257 polypeptide spots were analyzed in gels derived from 186 clones. Four structural mutations were detected by visual analysis of the gels. Computer analysis of gels corresponding to the mutant clones was also undertaken. At a spot size threshold of 200 spots to be matched using a computer algorithm, all four mutant polypeptides were detected. These results indicate the usefulness of the two-dimensional approach for mutagenesis studies at the protein level.

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An Algorithm for Comparing Two-Dimensional Electrophoretic Gels, with Particular Reference to the Study of Mutation

Skolnick

Neel

1986

Advances in Human Genetics 15

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Biochemical Approaches to Monitoring Human Populations for Germinal Mutation rates: I. Electrophoresis

Cited by 5 publications

References 38 publications

A pilot study of the use of placental cord blood samples in monitoring for mutational events

A pilot study of the use of placental cord blood samples in monitoring for mutational events

Detection of human somatic cell structural gene mutations by two‐dimensional electrophoresis

An Algorithm for Comparing Two-Dimensional Electrophoretic Gels, with Particular Reference to the Study of Mutation

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