Biochemical aspects of α-l-fucosidase in hepatocellular carcinoma

Leray, G; Deugnier, Yves; Jouanolle, Anne–Marie; Lehry, Dominique; Bretagne, Jean–François; Campion, Jean-Plerre; Brissot, Pierre; Treut, A. Le

doi:10.1002/hep.1840090214

Cited by 21 publications

(20 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, although alterations in the activity of tissue alpha- l -fucosidase have been described in different human tumors [14–18], none of these studies have analyzed the expression of the FUCA1 gene. Currently, we cannot determine which molecular mechanisms are leading to the decrease in FUCA1 expression that we found in colorectal tumors.…”

Section: Resultsmentioning

confidence: 99%

“…On the other hand, alterations in the activity levels of this enzyme have been described in different human malignancies. A diminished fucosidase activity has been reported in hepatocarcinoma [14,15] whereas an increased fucosidase activity has been found in endometrial, ovarian and cervical cancer [16,17] as well as in thyroid and gastric tumors [18]. Regarding CRC, a previous study from our laboratory demonstrated a decrease in the levels of alpha- l -fucosidase activity and amount of the enzymatic protein in tumoral tissue compared to healthy mucosa [19].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors

Otero-Estévez

Martínez‐Fernández

Vázquez-Iglesias

et al. 2013

IJMS

View full text Add to dashboard Cite

In previous studies we described a decreased alpha-l-fucosidase activity in colorectal tumors, appearing as a prognostic factor of tumoral recurrence. The aim of this work was to extend the knowledge about tissue alpha-l-fucosidase in colorectal cancer by quantifying the expression of its encoding gene FUCA1 in tumors and healthy mucosa. FUCA1 mRNA levels were measured by RT-qPCR in paired tumor and normal mucosa tissues from 31 patients. For the accuracy of the RT-qPCR results, five candidate reference genes were validated in those samples. In addition, activity and expression of alpha-l-fucosidase in selected matched tumor and healthy mucosa samples were analyzed. According to geNorm and NormFinder algorithms, RPLP0 and HPRT1 were the best reference genes in colorectal tissues. These genes were used for normalization of FUCA1 expression levels. A significant decrease of more than 60% in normalized FUCA1 expression was detected in tumors compared to normal mucosa (p = 0.002). Moreover, a gradual decrease in FUCA1 expression was observed with progression of disease from earlier to advanced stages. These findings were confirmed by Western blot analysis of alpha-l-fucosidase expression. Our results demonstrated diminished FUCA1 mRNA levels in tumors, suggesting that expression of tissue alpha-l-fucosidase could be regulated at transcriptional level in colorectal cancer.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors

Otero-Estévez

Martínez‐Fernández

Vázquez-Iglesias

et al. 2013

IJMS

View full text Add to dashboard Cite

show abstract

“…37 Phosphotyrosine interaction domain containing one (PID1) function as a growth-inhibitory gene in brain tumors 38 and is a potent intracellular inhibitor of the insulin signaling pathway during obesity in humans and mice. 39 Fucosidase, alpha-L-1, tissue (FUCA1) is a liposomal enzyme that degrades a variety of fucosecontaining fucoglycoconjugates and has been proposed as a promising tumor marker in the diagnosis 40 and prognosis 41 of hepatocellular carcinoma. Finally, G protein-coupled receptor 15 (GPR15) acts as a chemokine receptor for human immunodeficiency virus.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Different Case Definitions of Current Smoking on the Discovery of Smoking-Related Blood Gene Expression Signatures in Chronic Obstructive Pulmonary Disease

Obeidat

Ding

Fishbane

et al. 2016

NICTOB

View full text Add to dashboard Cite

Introduction: Smoking is the number one modifiable environmental risk factor for chronic obstructive pulmonary disease (COPD). Clinical, epidemiological and increasingly "omics" studies assess or adjust for current smoking status using only self-report, which may be inaccurate. Objective measures such as exhaled carbon monoxide (eCO) may also be problematic owing to limitations in the measurements and the relatively short half life of the molecule. In this study, we determined the impact of different case definitions of current cigarette smoking on gene expression in peripheral blood of patients with COPD. Methods: Peripheral blood gene expression from 573 former-and current-smokers with COPD in the ECLIPSE study was used to find genes whose expression was associated with smoking status. Current smoking was defined using self-report, eCO concentrations, or both. Linear regression was used to determine the association of current smoking status with gene expression adjusting for age, sex and propensity score. Pathway enrichment analyses were performed on genes with P < .001. Result: Using self-report or eCO, only two genes were differentially expressed between current and ex-smokers, with no enrichment in biological processes. When current smoking was defined using both eCO and self-report, four genes were differentially expressed (LRRN3, PID1, FUCA1, GPR15) with enrichment in 40 biological pathways related to metabolic processes, response to hypoxia and hormonal stimulus. Additionally, the combined definition provided better distributions of test statistics for differential gene expression. Conclusion: A combined phenotype of eCO and self report allows for better discovery of genes and pathways related to current smoking.

show abstract

“…So, the increased AFU levels in HCC patients is not related to liver regeneration or necrosis but probably associated with an increased synthesis of protein that leads to an increase in fucose turnover [53]. Nevertheless, this explanation is not supported by recent studies that show a decrease of AFU expression in tumoral liver tissues compared to normal tissues [54]. AFU measurement is useful in association with AFP in the early diagnosis of HCC [55].…”

Section: Human Carbonyl Reductasementioning

confidence: 94%

Serum Markers of Hepatocellular Carcinoma

et al. 2010

View full text Add to dashboard Cite

Despite the large number of studies devoted to the immunohistochemistry of HCC, at the present time, the absolute positive and negative markers for HCC are still lacking, and even those characterized by very high sensitivity and specificity do not have an universal diagnostic usefulness. Given the poor response to current therapies, a better understanding of the molecular pathways active in this disease could potentially provide new targets for therapy. However, AFP shows a low sensitivity, therefore other biomarkers have been developed to make an early diagnosis and improve patients' prognosis.

show abstract

Biochemical aspects of α-l-fucosidase in hepatocellular carcinoma

Cited by 21 publications

References 10 publications

Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors

Decreased Expression of Alpha-L-Fucosidase Gene FUCA1 in Human Colorectal Tumors

The Effect of Different Case Definitions of Current Smoking on the Discovery of Smoking-Related Blood Gene Expression Signatures in Chronic Obstructive Pulmonary Disease

Serum Markers of Hepatocellular Carcinoma

Contact Info

Product

Resources

About