Biochemical Assessment of Cellular Damage after Adipocyte Harvest

Lalikos, Janice F.; Li, Y Q; Roth, Timothy P.; Doyle, J. William; Matory, W. Earle; Wt, Lawrence

doi:10.1006/jsre.1997.5090

Cited by 79 publications

(44 citation statements)

References 11 publications

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“…Independent studies have determined that liposuction aspiration alone does not significantly alter the viability of isolated SVF cells. [41][42][43] Indeed, adherent stromal cells with characteristics of adipocyte progenitors can be found directly within the liposuction aspiration fluid, as well as in SVF derived from the tissue fragment digests. 44 However, when ultrasound-assisted liposuction is performed, the number of cells recovered from tissue digests is reduced, as is their proliferative capacity.…”

Section: Cell Isolation and Mechanical Devicesmentioning

confidence: 99%

Adipose-Derived Stem Cells for Regenerative Medicine

Gimble

Katz

Bunnell

2007

Circulation Research

2,083

1,573

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Abstract-The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue represents an abundant and accessible source of adult stem cells with the ability to differentiate along multiple lineage pathways. The isolation, characterization, and preclinical and clinical application of adipose-derived stem cells (ASCs) are reviewed in this article.

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Section: Cell Isolation and Mechanical Devicesmentioning

confidence: 99%

Adipose-Derived Stem Cells for Regenerative Medicine

Gimble

Katz

Bunnell

2007

Circulation Research

2,083

1,573

View full text Add to dashboard Cite

show abstract

“…The procedure generates finely minced tissue fragments whose size depends on the cannula's dimensions. Independent studies have determined that liposuction aspiration alone does not significantly alter the viability of isolated SVF cells [29][30][31]. Indeed, adherent stromal cells with characteristics of adipocyte progenitors can be found directly within the liposuction aspiration fluid, as well as in SVF derived from the tissue fragment digests [32].…”

Section: Isolation Of Mesenchymal Stem Cells From Adipose Tissuementioning

confidence: 99%

Adipose-derived stem cells: Isolation, expansion and differentiation☆

Bunnell

Flaat

Gagliardi

et al. 2008

Methods

914

713

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The emerging field of regenerative medicine will require a reliable source of stem cells in addition to biomaterial scaffolds and cytokine growth factors. Adipose tissue has proven to serve as an abundant, accessible and rich source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. There has been increased interest in Adipose-derived Stem Cells (ASCs) for tissue engineering applications. Here, methods for the isolation, expansion and differentiation of ASCs are presented and described in detail. While this article has focused on the isolation of ASCs from human adipose tissue, the procedure can be applied to adipose tissues from other species with minimal modifications.

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“…This stromalvascular fraction (SVF) consists of a heterogeneous cell population of which the adipose-derived adult stem (ADAS) cells are of significant interest for potential regenerative applications [2]. These multipotent cells [3] can be harvested through liposuction without altering their viability [4,5]. The objective in engineering adipose tissue is to shape a three-dimensional tissue substitute which prospers by biochemical, physical, and cellular cues and approximates the native tissue equivalent.…”

Section: Introductionmentioning

confidence: 99%

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

et al. 2008

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a b s t r a c tHistioconductive approaches to soft-tissue defects use scaffolds seeded with lineage-and tissue-specific progenitors to generate tissue which should reside in equilibrium with adjacent tissue. Scaffolds guide histiogenesis by ensuring cell-cell and cell-matrix interactions. Hyaluronic acid-based (HA) preadipocyte-seeded scaffolds were evaluated for their adipo-conductive potential and efficacy in humans. Preadipocytes were isolated from lipoaspirate material and seeded on HA scaffolds. The cellular biohybrid (ADIPOGRAFT Ò ) and an acellular control scaffold (HYAFF Ò 11) were implanted subcutaneously. At specific time points (2, 8 and 16 weeks) explants were analyzed histopathologically with immunohistochemistry. No adverse tissue effects occurred. Volume loss and consistent degradation of the HYAFF Ò 11 scaffolds compared to the ADIPOGRAFT Ò group indicated progressive tissue integration. No consistent histological differences between both groups were observed. By 8 weeks all void spaces within the scaffolds were filled with cells with pronounced matrix deposition in the ADIPOGRAFT Ò bio-hybrids. Here we show that HA scaffolds were stable cell carriers and had the potential to generate volumeretaining tissue. However, no adipogenic differentiation was observed within the preadipocyte-seeded scaffolds.

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Biochemical Assessment of Cellular Damage after Adipocyte Harvest

Cited by 79 publications

References 11 publications

Adipose-Derived Stem Cells for Regenerative Medicine

Adipose-Derived Stem Cells for Regenerative Medicine

Adipose-derived stem cells: Isolation, expansion and differentiation☆

Human clinical experience with adipose precursor cells seeded on hyaluronic acid-based spongy scaffolds

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