BIOCHEMICAL CHANGES OCCURRING DURING SPORULATION OF
            <i>BACILLUS CEREUS</i>

Gollakota, K.G.; Halvorson, H. O.

doi:10.1128/jb.79.1.1-8.1960

Cited by 52 publications

(30 citation statements)

References 16 publications

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“…As can be seen from Table 1, the rate of RNA-P synthesized initially during germination was about 0.5% of the amount synthesized by vegetative cells. After 100 min of outgrowth, 'Media used: GL is a germination medium (29); B is a rich medium for outgrowth (29); G is a rich medium for exponential vegetative growth (8).…”

Section: Resultsmentioning

confidence: 99%

Ribonucleic acid polymerase of germinating Bacillus cereus T

Hattori¹,

Ben-Ze’ev²,

Silberstein³

et al. 1975

J Bacteriol

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It appears that a de novo synthesis of the deoxyribonucleic acid-dependent ribonucleic acid-polymerase in Bacillus cereus T takes place fairly late in outgrowth, at the onset of the vegetative cycle. Therefore, the ribonucleic acid-polymerase used by germinating spores is the one carried on from sporulating cells. However, the sporal enzyme is less soluble that the vegetative one, and its "core" is bound to two extra peptides. This complexing to other molecules could play a role in the regulation of gene expression during germination.

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Section: Resultsmentioning

confidence: 99%

Ribonucleic acid polymerase of germinating Bacillus cereus T

Hattori¹,

Ben-Ze’ev²,

Silberstein³

et al. 1975

J Bacteriol

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“…Overall, our results help to define better the problem of the control of dipicolinic acid synthesis. Organisms and growth B. cereus T was grown in G medium (Gollakota & Halvorson, 1960) modified by the addition of 0.01 Mtris-HCI, pH7.6 (G-tris). Cultures were grown at 30°C as described by Levisohn & Aronson (1967).…”

mentioning

confidence: 99%

Regulation of dipicolinic acid biosynthesis in sporulating Bacillus cereus. Characterization of enzymic changes and analysis of mutants

Forman

Aronson

1972

Biochemical Journal

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Some of the early enzymes in the lysine-biosynthetic pathway also function for dipicolinic acid synthesis in sporulating Bacillus cereus T. 1. The first enzyme, aspartokinase, loses its sensitivity to feedback inhibition by lysing. This change occurs before the time of dipicolinic acid synthesis but at a time when diaminopimelic acid is required for spore cortex formation. 2. A possible regulatory change at a branch point in the pathway was studied by examining the properties of a key enzyme, dihydrodipicolinic acid reductase. No alteration in the feedback sensitivity or sedimentation rate of this enzyme could be detected during sporulation. 3. Two mutants producing heat-sensitive spores were analysed. Both produced spores that contained decreased amounts of dipicolinic acid. Although neither was a lysine auxotroph, they both had greatly decreased activities of certain lysine-biosynthetic enzymes in sporulating cells. 4. Starvation of cells for calcium also results in the production of spores that are heat-sensitive and contain less dipicolinic acid than the control. A decreased content of one of the lysine-biosynthetic enzymes, dihydrodipicolinic acid synthetase, in calcium-starved cells could account for the lower concentration of dipicolinic acid in the spores.

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“…Generally, rich media such as trypticase soy agar (TSA) do not promote sporulation in cultures kept refrigerated (C. Fermanian, unpublished data). Some chemicals, such as a-picolinic acid, a structural analogue of dipicolinic acid, a constituent of bacterial spores, inhibit sporulation without inhibiting growth or germination (Gollakota and Halvorson 1960). This acid inhibits sporulation only when it is added before the cells reached a certain age.…”

Section: Sporulation and Germinationmentioning

confidence: 99%

BACILLUS CEREUS PATHOGENICITY: A REVIEW

Fermanian¹,

Frémy²,

Lahellec³

1993

Rapid Methods Automation Mic

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Pathogenic Bacillus cereus can be routinely isolated and identified in the laboratory from foods and other sources. Typing of B. cereus strains implicated in food poisoning outbreaks is helpful for confirmation of the origin of the outbreak and for epidemiological studies. Data concerning vegetative growth and spores are given. Different types of toxin are produced by B. cereus in the course of its growth: a so‐called diarrheal enterotoxin and an emetic heat‐stable toxin; their biochemical characteristics and the systems used for their detection are reviewed. Different types of hemolysins and phospholipases C are also produced and may play a role in pathogenicity. Nongastrointestinal infections were also traced to this species.

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BIOCHEMICAL CHANGES OCCURRING DURING SPORULATION OF BACILLUS CEREUS

Cited by 52 publications

References 16 publications

Ribonucleic acid polymerase of germinating Bacillus cereus T

Ribonucleic acid polymerase of germinating Bacillus cereus T

Regulation of dipicolinic acid biosynthesis in sporulating Bacillus cereus. Characterization of enzymic changes and analysis of mutants

BACILLUS CEREUS PATHOGENICITY: A REVIEW

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