Biochemical characterization and application of a novel lectin from the cyanobacterium Lyngabya confervoides MK012409 as an antiviral and anticancer agent

El‐Fakharany, Esmail M.; Saad, Mabroka H.; Salem, Marwa S.; Sidkey, Nagwa M.

doi:10.1016/j.ijbiomac.2020.06.046

Cited by 26 publications

(16 citation statements)

References 60 publications

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“…Silk protein cytotoxicity was tested on both Vero cells and PBMCs using MTT assay [ 32 , 33 ]. Briefly, both Vero cells and PBMCs were seeded in 96-well cell culture plates at densities of 10 4 and 10 5 cells/well and incubated at 37 °C in 5% CO 2 for 24 h. After incubation, serial dilutions of the tested silk protein were incubated with Vero and PBMCs for 72 h. Serial dilutions of the HSV-1 and ADV7 standard drugs (ribavirin and acyclovir, respectively) were incubated with Vero cells for 72 h. At the end of incubation, 20 μL of 5 mg/mL MTT (Sigma, St. Louis, MO, USA) was added to each well and the plates were incubated at 37 °C for 3 h. After discarding MTT solution, 100 µL DMSO (dimethyl sulfoxide) was added and the dye intensity was quantified using the automated ELISA microplate reader adjusted to 570 nm to quantify the cell viability [ 26 , 34 ]. The effective safe concentration (EC 100 ) doses (at 100% cell viability) of the tested protein were estimated by the GraphPad Prism 9 InStat software.…”

Section: Methodsmentioning

confidence: 99%

“…There are many examples of traditional medicine such as the use of camel milk for HCV treatment [ 21 ], as camel milk contains many important proteins that play a crucial role in viral prevention [ 22 , 23 , 24 ]. In addition, there are many microbial metabolites are used as effective compounds in viral treatment, including mushroom and cyanobacterial lectins [ 25 , 26 , 27 , 28 ]. Medical usage of natural bioactive ingredients as a main source of treatment to date is well known, even considering the great contribution of chemotherapeutic drugs to modern therapy.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

El‐Fakharany

Abu‐Serie

Habashy

et al. 2022

JFB

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Bacterial polymeric silk is produced by Bacillus sp. strain NE and is composed of two proteins, called fibroin and sericin, with several biomedical and biotechnological applications. In the current study and for the first time, the whole bacterial silk proteins were found capable of exerting antiviral effects against herpes simplex virus type-1 (HSV-1), adenovirus type 7 (AD7), and hepatitis C virus (HCV). The direct interaction between bacterial silk-like proteins and both HSV-1 and AD7 showed potent inhibitory activity against viral entry with IC50 values determined to be 4.1 and 46.4 μg/mL of protein, respectively. The adsorption inhibitory activity of the bacterial silk proteins showed a blocking activity against HSV-1 and AD7 with IC50 values determined to be 12.5 and 222.4 ± 1.0 μg/mL, respectively. However, the bacterial silk proteins exhibited an inhibitory effect on HSV-1 and AD7 replication inside infected cells with IC50 values of 9.8 and 109.3 μg/mL, respectively. All these results were confirmed by the ability of the bacterial silk proteins to inhibit viral polymerases of HSV-1 and AD7 with IC50 values of 164.1 and 11.8 μg/mL, respectively. Similarly, the inhibitory effect on HCV replication in peripheral blood monocytes (PBMCs) was determined to be 66.2% at concentrations of 100 μg/mL of the bacterial silk proteins. This antiviral activity against HCV was confirmed by the ability of the bacterial silk proteins to reduce the ROS generation inside the infected cells to be 50.6% instead of 87.9% inside untreated cells. The unique characteristics of the bacterial silk proteins such as production in large quantities via large-scale biofermenters, low costs of production, and sustainability of bacterial source offer insight into its use as a promising agent in fighting viral infection and combating viral outbreaks.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

El‐Fakharany

Abu‐Serie

Habashy

et al. 2022

JFB

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“…To evaluate the antioxidant activity of P, G, P + G, C, and P + C, their scavenging activity against both ABTS and DPPH free radicals were investigated as described by El-Fakharany et al [46] Various concentrations of each sample/compound (12.5, 25, 50, 100, 150, and 200 μg/ml) were tested in triplicates. In addition, ascorbic acid was used as positive control.…”

Section: Antioxidant Assaymentioning

confidence: 99%

New Gd(I)/Cs(III) complexes of benzil‐based thiocarbohydrazone macrocyclic ligand: Chemical synthesis, characterization, and study their biological effectiveness as antibacterial, antioxidant, and antiviral additives for polyurethane surface coating

Eliwa

Elgammal

Sharaf

et al. 2022

Applied Organom Chemis

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Germs are transmitted in different ways and remain viable or infectious on metal, glass, wood, fabrics, and plastic surfaces for prolonged periods of time. Thus, sterilizing commonly handled everyday objects and public places with high contamination potential is a major global strategy to combat the spread of pathogenic microorganisms. Consequently, our response is development of durable surface‐active coating embedded with new Gd(I)/Cs(III) complexes that derived from condensation of thiocarbohydrazide and benzil precursors. Chemical structures of these complexes were elucidated by Fourier transform infrared (FTIR), electron impact ionization (EI‐MS), 1H nuclear magnetic resonance (NMR), elemental analysis, and scanning electron microscopy (SEM). Next, the well‐established complexes were physically mixed with wide‐range applicable polyurethane (PU) varnish as potential biocide agents. With laboratory scale, coating material was loaded into stainless steel and wood panels to evaluate the physical and mechanical properties. In comparison with the blank formulation, our additives enhanced the gloss levels of the developed PU from 75 to 80 gloss unit (GU), and the scratch hardness was increased to reach >2 kg. Meanwhile, all the coated films passed the flexibility bend test and showed none flaking in mechanical adhesion test. Consequently, Gd(I) and Cs(III) metal complexes did not show any undesirable side effects on PU coating performance. Concerning biological screening, macrocyclic Gd(I) and Cs(III) complexes as well as the reformulated PU varnish were assayed for their antibacterial, antioxidant, and antiviral activities. Antibacterial activity of the developed PU was slightly increased than the individual treatments of Gd(I)/Cs(III) complexes by 1–2 mm, although the blank PU showed no obvious activity against all the tested bacterial strains. Antiviral overall results indicated that the Gd(I) and Cs(III) complexes demonstrated higher activity than the developed PU samples against ADeno‐7, CV‐B4, and HSV‐1 viruses in all modes of action, and hence these coordinated compounds could be promising virucidal agents with good biocompatibility.

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“…The fruiting bodies of oyster mushrooms are of high nutritional and healthpromoting value. In addition, many species belonging to the Pleurotus genus have been used as sources of substances with documented medicinal properties (Golak-Siwulska et al, 2018;Gregori et al, 2007) Anticarcinogenic and antitumor activities of lectins are due to diverse mechanisms mostly as direct cytotoxicity on malignant cells, inducing reductions in certain tumors, stimulation of normal growth in tumor cells, inhibition of the neoplastic effects of chemotherapy and radiation, and increasing immunogenicity of tumor cells (El-Fakharany et al 2020). Additionally, many anticancer lectins usually possess low cytotoxicity to non-transformed cells.…”

Section: Introductionmentioning

confidence: 99%

Purification, Molecular and Biochemical Characterization and Biological Applications of Hemagglutinating Lectin With Anticancer Activities From Pleurotus Ostreatus

Kamel¹,

Khalil²,

Atalla³

et al. 2021

PLANT ARCHIVES

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Three Pleurotus species were screened for their ability to synthesize mycelial, culture filtrate and fruiting bodies lectins. All tested Pleurotus species were able to synthesize mycelial and fruiting bodies lectins but with different degree of activity, while no extracellular lectin was detected. P. ostreatus Lectin (POL) from its fruiting bodies recorded the highest activity, so it was selected for further studies. Partial sequencing of lectin gene and partial amino acid sequencing were performed, resulting in 267 amino acids. POL purification involved a combination of (NH 4 ) 2 SO 4 precipitation along with two anion-chromatographic steps using Q-Sepharose column and Mono Q column. POL appeared as a single band with a molecular mass of 29 kDa on SDS-PAGE. Biochemical and biological activities of pure POL were compared with crude extract from the same organism. POL activity was inhibited by xylose with a MIC of 0.1 M. It was active up to 70ºC and pH 11 then its activity completely disappeared. POL activity was inhibited by different concentrations of Hg 2+ , Zn 2+ , Cu 2+ , Mn 2+ , Ca 2+ , Na + and K + ions. POL manifested antibacterial activity, but showed no antifungal activity. It exhibited anticancer activity toward human hepatocellular and colorectal carcinoma with IC 50 of 424 and 270 µg/ml, respectively.

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Biochemical characterization and application of a novel lectin from the cyanobacterium Lyngabya confervoides MK012409 as an antiviral and anticancer agent

Cited by 26 publications

References 60 publications

Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

Inhibitory Effects of Bacterial Silk-like Biopolymer on Herpes Simplex Virus Type 1, Adenovirus Type 7 and Hepatitis C Virus Infection

New Gd(I)/Cs(III) complexes of benzil‐based thiocarbohydrazone macrocyclic ligand: Chemical synthesis, characterization, and study their biological effectiveness as antibacterial, antioxidant, and antiviral additives for polyurethane surface coating

Purification, Molecular and Biochemical Characterization and Biological Applications of Hemagglutinating Lectin With Anticancer Activities From Pleurotus Ostreatus

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