Biochemical Characterization and Quantitation of the Collagenous Components of Urethral Stricture Tissue

Baskin, Laurence S.; Constantinescu, S; Howard, Pamela S.; McAninch, Jack W.; Ewalt, David H.; Duckett, John W.; Snyder, Howard M.; Macarak, Edward J.

doi:10.1016/s0022-5347(17)35572-6

Cited by 132 publications

(99 citation statements)

References 20 publications

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“…In US pathogenesis, excessive fibrosis occurs and is characterized by an increase in collagen type I and a decrease in collagen type III (Baskin et al 1993). Histologically, US presents as collagen-rich connective tissue and fibroblasts that replace the corpus spongiosum that surrounds the urethra (MacAninch et al 1988).…”

Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Increased Expression of Connective Tissue Growth Factor in Patients with Urethral Stricture

Zhang

Shi

Wei

et al. 2008

Tohoku J. Exp. Med.

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Urethral stricture (US) is a urologic disorder with a high morbidity and recurrence rate, and may arise due to external trauma, surgical procedures or urethritis. The pathogenesis of US is closely associated with scar formation, including excessive collagen-rich connective tissues. Previous studies have shown that connective tissue growth factor (CTGF) plays a key role in the fibrosis process of many tissues and organs, such as kidney and lung. We investigated the variation of CTGF expression in urethral tissues from 12 patients with US and normal urethral tissues. The stricture length range was 1 to 2.5 cm, and 4 patients underwent repeated urethral dilatation. The normal urethral tissues were obtained from 6 patients with heart disease, who were suffering from brain death. The expression of CTGF mRNA and protein was analyzed by real-time PCR, immunohistochemistry, and western blotting analyses. Results showed that the expression level of CTGF mRNA was significantly increased in US patients compared with the control. In addition, US patients who have longer history and more repeated surgical procedures have extremely high CTGF mRNA levels and its protein expression, while the age of patients, position of injury and stricture length were not found obvious relation with CTGF expression. Moreover, the degree of collagen deposition and muscle fiber proliferation in the submucosa is consistent with the increase in CTGF expression. In conclusion, our data suggest that the up-regulation of CTGF expression may be responsible for the fibrosis process of urethral tissues in US patients.

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Section: Immunohistochemistry (Ihc)mentioning

confidence: 99%

Increased Expression of Connective Tissue Growth Factor in Patients with Urethral Stricture

Zhang

Shi

Wei

et al. 2008

Tohoku J. Exp. Med.

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“…Thus, the fibroelastic elements (collagen and elastin) combine to form key structures in compliant tissues [5,8]. The human spongy urethra has a significant collagen content [2,3,9], and through the interaction of elastic fibres with collagen it was suggested that elastic fibres are important for urethral compliance, although this issue has still not been properly studied in the fetal urethra.…”

Section: Introductionmentioning

confidence: 99%

The concentration of elastic fibres in the male urethra during human fetal development

et al. 2004

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Morphometric values were assessed by the point-counting method. The volumetric density (Vv) of elastic fibres was correlated with fetal age. RESULTSAt 15 weeks the elastic fibres were sparse and homogeneously distributed. The size and thickness of elastic fibres increased with age, mainly in the third trimester of gestation. Elastic fibres formed a randomly orientated network in the trabeculae of the corpus spongiosum. The mean ( SEM ) Vv of elastic fibres in the spongy urethra was 5.2 (0.4)% in the fetus at 15 weeks and 14.8 (1.0)% at 36 weeks. In the urethra of the placematched young man the Vv was 19.0 (1.3)%.

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“…Changes in the elements of the extracellular matrix of an organ also change these proprieties (Peters et al, 1997;da Silva et al, 2002). The observed human male urethras presented a uniform gross anatomical appearance, while changes in the extracellular matrix could be noted in its various segments (membranous, spongy and glandular), justifying the functional (Hsu et al, 1994;da Silva et al) and pathological differences (Singh & Blandy, 1976;Baskin et al, 1993;da Silva et al). A significant increase of reticular fibers was found.…”

Section: Discussionmentioning

confidence: 78%

High Concentration of Reticular Fibers in Periurethral Region of the Prostatic Urethra from Patients with Benign Prostatic Hyperplasia as Revealed by Stereology

et al. 2012

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SUMMARY:The goal of this investigation was to analyze and quantify changes of the reticular fibers in the prostatic urethra of patients with benign prostatic hyperplasia (BPH) and compare with a control group. Prostatic urethra tissue samples were obtained from ten patients (age range 65 to 79 years, mean 66) with clinical symptoms of bladder outlet obstruction who had undergone open prostatectomy. The ten control group samples (urethral tissue samples from the transitional zone) were collected from prostates obtained during autopsy of accidental death adults of less than 25 years. The Vv of the reticular fibers was determined with stereologic methods from 25 random fields per sample using the point-count method with a M-42 grid test system. The quantitative data were analyzed using the KolmogorovSmirnov and Mann-Whitney U tests. The Vv (mean±SD) in the control and BPH groups respectively were: 23.4± 1.8 and 30.3 ± 1.2 (0.001). BPH cause significant increase of reticular fibers in prostatic urethra.

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Biochemical Characterization and Quantitation of the Collagenous Components of Urethral Stricture Tissue

Cited by 132 publications

References 20 publications

Increased Expression of Connective Tissue Growth Factor in Patients with Urethral Stricture

Increased Expression of Connective Tissue Growth Factor in Patients with Urethral Stricture

The concentration of elastic fibres in the male urethra during human fetal development

High Concentration of Reticular Fibers in Periurethral Region of the Prostatic Urethra from Patients with Benign Prostatic Hyperplasia as Revealed by Stereology

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