Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

Wanmolee, Wanwitoo; Sornlake, Warasirin; Rattanaphan, Nakul; Suwannarangsee, Surisa; Laosiripojana, Navadol; Champreda, Verawat

doi:10.1186/s12896-016-0312-7

Cited by 27 publications

(10 citation statements)

References 35 publications

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“…In another set of experiments, the effect of supplementing 5 mg of Accellerase ® 1500 cellulolytic enzyme with 5 mg of Accellerase ® XY xylanolytic enzyme was studied. Although Accellerase 1500 primarily contains cellulase and β-glucosidase activities, Accellerase ® XY has mostly xylanases and β-xylosidase activities [16]. As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Impacts of cellulase deactivation at the moving air–liquid interface on cellulose conversions at low enzyme loadings

Bhagia

Wyman

Kumar

2019

Biotechnol Biofuels

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Background We recently confirmed that the deactivation of T. reesei cellulases at the air–liquid interface reduces microcrystalline cellulose conversion at low enzyme loadings in shaken flasks. It is one of the main causes for lowering of cellulose conversions at low enzyme loadings. However, supplementing cellulases with small quantities of surface-active additives in shaken flasks can increase cellulose conversions at low enzyme loadings. It was also shown that cellulose conversions at low enzyme loadings can be increased in unshaken flasks if the reactions are carried for a longer time. This study further explores these recent findings to better understand the impact of air–liquid interfacial phenomena on enzymatic hydrolysis of cellulose contained in Avicel, Sigmacell, α-cellulose, cotton linters, and filter paper. The impacts of solids and enzyme loadings, supplementation with nonionic surfactant Tween 20 and xylanases, and application of different types of mixing and reactor designs on cellulose hydrolysis were also evaluated. Results Avicel cellulose conversions at high solid loading were more than doubled by minimizing loss of cellulases to the air–liquid interface. Maximum cellulose conversions were high for surface-active supplemented shaken flasks or unshaken flasks because of low cellulase deactivation at the air–liquid interface. The nonionic surfactant Tween 20 was unable to completely prevent cellulase deactivation in shaken flasks and only reduced cellulose conversions at unreasonably high concentrations. Conclusions High dynamic interfacial areas created through baffles in reactor vessels, low volumes in high-capacity vessels, or high shaking speeds severely limited cellulose conversions at low enzyme loadings. Precipitation of cellulases due to aggregation at the air–liquid interface caused their continuous deactivation in shaken flasks and severely limited solubilization of cellulose. Electronic supplementary material The online version of this article (10.1186/s13068-019-1439-2) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Impacts of cellulase deactivation at the moving air–liquid interface on cellulose conversions at low enzyme loadings

Bhagia

Wyman

Kumar

2019

Biotechnol Biofuels

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“…Previous studies have suggested that cellobiohydrolase gene expression and the cellulolytic activity profile vary depending on the carbon source used in induction experiments in fungi [ 10 , 19 , 44 ]. But the regulatory mechanism acts differently in different fungi.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, Chaetomium globosum is a saprophytic fungus with a high capability for degrading plant materials, such as wheat straw, coffee pulp, and oil palm empty fruit bunch (OPEFB) fiber to produce cellulase [ 9 ]. Recently, the cellulose- degrading enzyme system of C. globosum has been studied, and the results suggest an excellent potential for developing a cellulase-producing strain for on-site enzyme production [ 10 ]. However, the transcriptional regulation mechanism of cellulase production in C. globosum is still not clear.…”

Section: Introductionmentioning

confidence: 99%

Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum

Liu

Hao

et al. 2018

Microb Cell Fact

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BackgroundThe canonical heterotrimeric G protein-cAMP/PKA pathway regulates numerous cellular processes in filamentous fungi. Chaetomium globosum, a saprophytic fungus, is known for producing many secondary metabolites, including cytotoxic chaetoglobosin A (ChA), as well as abundant cellulase and xylanase.ResultsHere we report on the functional characterization of this signaling pathway in C. globosum. We blocked the pathway by knocking down the putative Gα-encoding gene gna1 (in the pG14 mutant). This led to impaired cellulase production and significantly decreased transcription of the major cellulase and xylanase genes. Almost all the glycohydrolase family genes involved in cellulose degradation were downregulated, including the major cellulase genes, cel7a, cel6a, egl1, and egl2. Importantly, the expression of transcription factors was also found to be regulated by gna1, especially Ace1, Clr1/2 and Hap2/3/5 complex. Additionally, carbon metabolic processes including the starch and sucrose metabolism pathway were substantially diminished, as evidenced by RNA-Seq profiling and quantitative reverse transcription (qRT)-PCR. Interestingly, these defects could be restored by simultaneous knockdown of the pkaR gene encoding the regulatory subunit of cAMP-dependent PKA (in the pGP6 mutant) or supplement of the cAMP analog, 8-Br-cAMP. Moreover, the Gα-cAMP/PKA pathway regulating cellulase production is modulated by environmental signals including carbon sources and light, in which VelB/VeA/LaeA complex and ENVOY probably work as downstream effectors.ConclusionThese results revealed, for the first time, the positive role of the heterotrimeric Gα-cAMP/PKA pathway in the regulation of cellulase and xylanase utilization in C. globosum.Electronic supplementary materialThe online version of this article (10.1186/s12934-018-1008-6) contains supplementary material, which is available to authorized users.

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“…In this study, a lignocellulose-degrading enzyme system of Chaetomium globosum BCC5776 (CG-Cel) was characterized for its activity and proteomic profiles, and synergism with accessory enzymes. The highest cellulose productivity of 0.40 FPU/mL was found for CG-Cel under the optimized submerged fermentation conditions on 1% (w/v) EPFB (empty palm fruit bunch), 2% microcrystalline cellulose (Avicel®) and 1% soybean meal (SBM) at 30 °C, pH 5.8 for 6 d. CG-Cel worked optimally at 50-60 °C in an acidic pH range 78 .…”

Section: Introductionmentioning

confidence: 98%

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

Kumar¹,

Saini²,

Meenakshi³

et al. 2018

J. Pharm. Sci. Innov.

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In the past, the beneficial effects of fermented fruits were unknown, and so people primarily used fermentation to preserve fruits, enhance shelf life, and improve flavor. Fermentation of fruits has been adopted over many generations, primarily due to their commercial significance. Edible tropical fruits such as, cashew apple, mangoes, papaya, pineapple, litchi, guava, bael, banana, pomegranate, jamun and palm etc. with high export potential have their origin in the tropics and require rather suitable climatic conditions; and can not tolerate environmental stress. Most of the tropical fruits are important sources of antioxidants, vitamins and minerals and form a very healthy part of a diet. An effective utilisation of inferior grade and over-ripe fruits and processing them into fermented beverages has been revealed as a new and promising alternative to generate extra revenues whilst conducting a sustainable exploitation of wastes. In addition, microorganisms contributing to the fermentation process have recently been associated with many health benefits, and so these microorganisms have become another focus of attention. This paper reviews the production techniques and beneficial health effects of different fermented fruits. Scientific advances in the emerging area of functional beverages; as they provide a health benefit beyond the basic nutritional functions also reported in this paper.

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Biochemical characterization and synergism of cellulolytic enzyme system from Chaetomium globosum on rice straw saccharification

Cited by 27 publications

References 35 publications

Impacts of cellulase deactivation at the moving air–liquid interface on cellulose conversions at low enzyme loadings

Impacts of cellulase deactivation at the moving air–liquid interface on cellulose conversions at low enzyme loadings

Regulation of the Gα-cAMP/PKA signaling pathway in cellulose utilization of Chaetomium globosum

Emerging Applications of Fermented Fruit Enzyme Beverages as Nutraceutical, Functional Food, and Designer Food Products: A Review

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