Biochemical characterization of a novel glucose isomerase from Anoxybacillus gonensis G2T that displays a high level of activity and thermal stability

Karaoglu, Hakan; Yanmis, Derya; Şal, Fulya Ay; Çelik, Ayhan; Çanakçı, Sabriye; Beldüz, Ali Osman

doi:10.1016/j.molcatb.2013.08.019

Cited by 39 publications

(40 citation statements)

References 41 publications

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“…Strains, vectors, and media E. coli BL21 (DE3):pLysS, pET28(a-c)+ were supplied by Karadeniz Technical University, Molecular Biology Laboratory. E. coli containing recombinant plasmids were cultured according to the method described (Karaoglu et al, 2013). 2.3.…”

Section: Substrates and Chemicalsmentioning

confidence: 99%

“…PCR reactions were performed according to the method described by Karaoglu et al (2013). E. coli BL21 cells containing pAgoG2XI-his or pAgoG2XI were grown to an optimum density of about 0.6 at 600 nm.…”

Section: Cloning and Overexpression Of Xyla Genementioning

confidence: 99%

“…This is the reason why XI is also known as glucose isomerase (Jensen and Rugh, 1987;De Raadt et al, 1994). The fact that the enzyme isomerizes xylose to xylulose means that it could be used industrially for producing ethanol from hemicellulose (Wang et al, 1980;Ertunga et al, 2007;Karaoglu et al, 2013). Xylose, one of the major fermentable sugars in nature, is, after glucose, the second most abundant sugar in lignocellulosic biomass.…”

Section: Introductionmentioning

confidence: 99%

“…The enzyme has been isolated from many microorganisms and is well studied (Chen, 1980;Schellenberg et al, 1984;Wilhelm and Hollenberg, 1985;Saari et al, 1987;Amore and Hollenberg, 1989;Kikuchi et al, 1990;Dekker et al, 1991). Besides its commercial importance, XI is also an ideal enzyme for studying structure-function relationships from an academic perspective (Ertunga et al, 2007;Karaoglu et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T

Yanmis¹,

Karaoglu²,

Çolak³

et al. 2014

Turk J Biol

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IntroductionXylose isomerase (XI) (D-xylose ketol-isomerase E.C 5.3.1.5) catalyzes the isomerization of D-xylose into xylulose as the first step of xylose metabolism in many microorganisms (Wovcha et al., 1983). It is also responsible for catalyzing the isomerization of glucose to fructose in vitro, and is an important enzyme in the food industry, used in the production of high fructose corn syrup. This is the reason why XI is also known as glucose isomerase (Jensen and Rugh, 1987;De Raadt et al., 1994). The fact that the enzyme isomerizes xylose to xylulose means that it could be used industrially for producing ethanol from hemicellulose (Wang et al., 1980;Ertunga et al., 2007;Karaoglu et al., 2013). Xylose, one of the major fermentable sugars in nature, is, after glucose, the second most abundant sugar in lignocellulosic biomass. Efficient fermentation of xylose is a necessary step in developing economically viable processes for producing biofuels, such as ethanol, from biomass (Zeikus, 1996;Schenck, 2000). For this reason, many XI genes appropriate for industrial applications were transferred to Saccharomyces cerevisiae

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Section: Substrates and Chemicalsmentioning

confidence: 99%

Section: Cloning and Overexpression Of Xyla Genementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of a novel xylose isomerase from Anoxybacillus gonensis G2T

Yanmis¹,

Karaoglu²,

Çolak³

et al. 2014

Turk J Biol

View full text Add to dashboard Cite

show abstract

“…Strains, vectors and media E. coli JM101; E. coli BL21 (DE3); pET28a(+) were gently supplied from Molecular Biology Laboratory, Karadeniz Technical University, Turkey. E. coli containing recombinant plasmids was cultured according to the method of Karaoglu [19]. The Wizard Genomic DNA Purification Kit, Wizard plus SV Minipreps DNA Purification System, Taq DNA polymerase, dNTP's, and all of the restriction enzymes were purchased from Promega Corp. (Madison, WI, USA).…”

Section: Methodsmentioning

confidence: 99%